Genotype distribution of extended Spectrum beta-Lactamase producing Escherichia coli and Klebsiella pneumoniae.

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dc.contributor.authorDagi, Hatice Turk
dc.contributor.authorAl Dulaimi, Dhay Ali Azeez
dc.contributor.authorKus, Halit
dc.contributor.authorSeyhan, Tuba
dc.contributor.authorFindik, Duygu
dc.contributor.authorTuncer, Inci
dc.contributor.authorArslan, Ugur
dc.date.accessioned2020-03-26T19:06:03Z
dc.date.available2020-03-26T19:06:03Z
dc.date.issued2015
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractExtended-spectrum beta-lactamase (ESBL) production is the most important cause of beta-lactam resistance in Gram-negative bacteria. Although it may also be found in other Gram-negative bacteria, ESBL is most commonly produced by Escherichia coli and Klebsiella pneumoniae strains. In this study, we aimed to investigate the distribution of beta-lactamase genes in ESBL-producing E. coli and K. pneumoniae strains. One hundred and twenty isolates of E. coli and K. pneumoniae isolated from clinical samples were used in this study. The identification and the antibiotic susceptibility tests were performed by VITEK 2 system in accordance with the manufacturer's instructions. ESBL production was determined accoring to Clinical and Laboratory Standards Institute guidelines. The DNA isolation was performed with a commercial kit following company recommendations. (TEM)-T-bla, (SHV)-S-bla and (CTX)-C-bla-M genes were amplified by multiplex PCR with specific primers. Of the 120 isolates collected, 84 isolates were of E. coli and 36 isolates were of K. pneumoniae. (TEM)-T-bla gene was the most prevalent type (85.8%) followed by (CTX)-C-bla-M (83.3%) and (SHV)-S-bla (24.2%). No blaSHV gene was detected in the E. coli strains. Among 120 ESBL-producing strains, 10.8% were susceptible to cefepime, 10.0% to ceftazidime, while 5.0% to ceftriaxone. In conclusion, (TEM)-T-bla gene was the most frequently encountered ESBL of E. coli and K. pneumonia in our hospital. Further molecular surveillance and epidemiological studies of such resistant bacteria are recommended for monitoring and controlling the spread of ESBL producing strains.en_US
dc.identifier.endpage238en_US
dc.identifier.issn0970-938Xen_US
dc.identifier.issn0976-1683en_US
dc.identifier.issue2en_US
dc.identifier.startpage235en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/32192
dc.identifier.volume26en_US
dc.identifier.wosWOS:000354721900007en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherALLIED ACADen_US
dc.relation.ispartofBIOMEDICAL RESEARCH-INDIAen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectExtended-spectrum beta-lactamaseen_US
dc.subjectEscherichia colien_US
dc.subjectKlebsiella pneumoniaeen_US
dc.subjectTEMen_US
dc.subjectSHVen_US
dc.subjectCTX-Men_US
dc.titleGenotype distribution of extended Spectrum beta-Lactamase producing Escherichia coli and Klebsiella pneumoniae.en_US
dc.typeArticleen_US

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