Sağlıklı gebelerin perifer kan lenfositlerinde bazı AgNOR parametreleri ve mikronükleus sıklığı ile alfa naftil asetat esteraz ve asit fosfataz aktivitelerinin belirlenmesi
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Dosyalar
Tarih
2010
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi Sağlık Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışma, bayanlarda hamileliğin perifer kan lenfositlerinin alfa naftil asetat esteraz (ANAE) ve asit fosfataz (ACP-az) aktiviteleri ile perifer kan lenfosit oranları üzerindeki etkilerinin belirlenmesi amacıyla yapıldı. Ayrıca perifer kan lenfositlerinde mikronükleus (MN) sıklığı ile bazı AgNOR parametreleri de belirlendi. Hamile bayanlar Kontrol; I. trimester; II. trimester ve III. trimester olmak üzere 4 gruba ayrıldılar (n= 10). En düşük ANAE-pozitif lenfosit oranı II. trimesterde tespit edilirken gruplar arasındaki farklar istatistiksel açıdan önemli bulundu. I. ve III. trimesterde ACP-az pozitif lenfosit oranlarında istatistiksel olarak önemli düşüşler gözlendi. Hamilelik süresince perifer kan lenfosit oranlarında belirgin düşüşler dikkati çekerken (p<0,05) en yüksek null hücre oranı I. trimesterde tespit edildi. MN sıklığında hamilelik süresince belirgin artışlar gözlendi (p<0,05). AgNOR sayılarında gruplar arasında fark tespit edilmezken; AgNOR alanı/Çekirdek alanı oranının hamilelikle birlikte artarak en yüksek değerine III. trimesterde ulaştığı görüldü (p<0,05). Trimesterler arasında bazı farklar olsa da hamileliğin ANAE- ve ACP-az pozitif perifer kan lenfosit oranlar ile perifer kan lenfosit oranı ile MN sıklığı ve bazı AgNOR parametrelerini etkilediği sonucuna varıldı.
This study was performed to determine the effects of pregnancy on the activities of alpha- naphthyl acetate esterase (ANAE) and acid phosphatase (ACP-ase) of the peripheral blood lymphocytes (PBL) and PBL percentages in pregnant women. Micronucleus frequency (MN) and some AgNOR parameters of PBL were also estimated. Women divided into four groups as Control; Trimester I (TRI); Trimester II (TRII) and Trimester III (TRIII) (n= 10 for each group). The lowest ANAE (+) lymphocytes percentage was determined in TRII and the differences between the groups were statistically important (p<0,05). There was a statistically significant decrease in the proportions of the ACP-ase (+) lymphocytes in TRI and TRIII. A dramatic reduction in the percentage of PBL was recorded during pregnancy (p<0,05), whereas the highest null cell rates were found in TRI. A distinct increasing was observed in MN frequency during pregnancy (p<0,05). There was no difference in mean AgNOR counts between the groups, whereas AgNORs area/nucleus area increased during pregnancy and it gained its highest value in TRIII. It was concluded that the ANAE- and ACP-ase positive PBL proportions, PBL percentages, MN frequency and some AgNOR parameters were affected by pregnancy although there were some differences among the TRs.
This study was performed to determine the effects of pregnancy on the activities of alpha- naphthyl acetate esterase (ANAE) and acid phosphatase (ACP-ase) of the peripheral blood lymphocytes (PBL) and PBL percentages in pregnant women. Micronucleus frequency (MN) and some AgNOR parameters of PBL were also estimated. Women divided into four groups as Control; Trimester I (TRI); Trimester II (TRII) and Trimester III (TRIII) (n= 10 for each group). The lowest ANAE (+) lymphocytes percentage was determined in TRII and the differences between the groups were statistically important (p<0,05). There was a statistically significant decrease in the proportions of the ACP-ase (+) lymphocytes in TRI and TRIII. A dramatic reduction in the percentage of PBL was recorded during pregnancy (p<0,05), whereas the highest null cell rates were found in TRI. A distinct increasing was observed in MN frequency during pregnancy (p<0,05). There was no difference in mean AgNOR counts between the groups, whereas AgNORs area/nucleus area increased during pregnancy and it gained its highest value in TRIII. It was concluded that the ANAE- and ACP-ase positive PBL proportions, PBL percentages, MN frequency and some AgNOR parameters were affected by pregnancy although there were some differences among the TRs.
Açıklama
Anahtar Kelimeler
Asetatlar, Acetates, Asit fosfataz, Acid phosphatase, Gebelik, Pregnancy, Lenfositler, Lymphocytes, Mikroçekirdek, Micronucleus
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Akbulut, B. (2010). Determination of the some AgNOR parameters, micronucleus frequency, activity of alpha naphthyl acetate esterase and acid phosphatase of peripheral blood lymphocytes in healty pregnant women. Selçuk Üniversitesi, Yayımlanmış yüksek lisans tezi, Konya.