Pleurotus ostreatus’un Misel Gelişmesine Sitokininlerin Etkisi
Yükleniyor...
Dosyalar
Tarih
2019
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi Fen Fakültesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada Pleurotus ostreatus’un misel gelişimi üzerine sitokinin olarak 6N-benzil adenin (BA) ve kinetin (Kin)’in etkisi araştırılmıştır. Besi yeri olarak malt ekstrakt agar (MEA) ve agar agar (AA) kullanılmıştır. Malt ekstrakt agar ve agar agara sitokininlerin %0.1, 0.3 ve 0.5 konsantrasyonları ilave edilerek otoklavda steril hale getirilmiştir. Ortamlar biyogüvenlik kabini içerisinde steril edilmiş petrilere dökülerek katılaşmaları sağlanmıştır. Besi yerlerine misel ihtiva eden 1 cm çapında kültür ilavesi yapılmış ve etüve alınmıştır. Belli zaman aralıklarıyla miselyum gelişmeleri alansal olarak çizilmiştir. Çalışma, miselyumun petrinin toplam alanını dolduruncaya kadar devam ettirilmiştir. Çalışma sonucunda her iki besiyerindeki kontrol gruplarına göre, MEA+%0.5 Kin ile AA+%0.5 BA içeren petrilerde diğer kombinasyonlara göre daha erken bir gelişme tespit edilmiştir. MEA besiyerinde gelişme sık-yoğun iken AA besiyerinde zayıf-seyrek bir gelişme gözlenmiştir.
In this study, the effect of 6-benzyl adenine (BA) and kinetin (Kin), known as cytokinin, on the development of micelle of Pleurotus ostreatus was investigated. Malt extract agar (MEA) and agar agar (AA) were used as a feedstock. By adding 0.1%, 0.3% ve 0.5% doses of cytokinins to MEA and AA were sterilized in the autoclave. The nutrients were poured into the pre-sterilized petri dishes in the biosafety cabinet and solidified. 1 cm diameter mushroom culture was added to the feedstock and petri dishes were taken to the incubator. The development of mycelium at certain time intervals has been drawn. Work continued until the mycelium had filled the total surface area of the feedstock. According to the experimental results, it was found that the earlier development has been detected from the petri dishes containing with MEA added with 0.5% Kin and AA added with 0.5% BA, compared to the control groups on both feedstock. While growth in MEA medium was frequent-dense, weak-sparse development was observed in AA medium.
In this study, the effect of 6-benzyl adenine (BA) and kinetin (Kin), known as cytokinin, on the development of micelle of Pleurotus ostreatus was investigated. Malt extract agar (MEA) and agar agar (AA) were used as a feedstock. By adding 0.1%, 0.3% ve 0.5% doses of cytokinins to MEA and AA were sterilized in the autoclave. The nutrients were poured into the pre-sterilized petri dishes in the biosafety cabinet and solidified. 1 cm diameter mushroom culture was added to the feedstock and petri dishes were taken to the incubator. The development of mycelium at certain time intervals has been drawn. Work continued until the mycelium had filled the total surface area of the feedstock. According to the experimental results, it was found that the earlier development has been detected from the petri dishes containing with MEA added with 0.5% Kin and AA added with 0.5% BA, compared to the control groups on both feedstock. While growth in MEA medium was frequent-dense, weak-sparse development was observed in AA medium.
Açıklama
Anahtar Kelimeler
Pleurotus ostreatus, Kinetin, Benzil adenin, Misel gelişmesi, Mycelium development
Kaynak
Selçuk Üniversitesi Fen Fakültesi Fen Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
45
Sayı
2
Künye
Ay, Z., Kaşık, G., Alkan, S . (2019), Pleurotus ostreatus’un misel gelişmesine sitokininlerin etkisi. Selçuk Üniversitesi Fen Fakültesi Fen Dergisi, 45, (2), 95-102.
