The effects of oleuropein on lung and heart injury in cecal ligation and puncture - induced sepsis
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Dosyalar
Tarih
2020
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Sepsis öldürücü ciddi bir klinik hastalıktır. Sepsis durumunda oksidatif stres inflamasyonda önemli bir rol oynar. Bu nedenle çalışmada oleuropeinin (OLE) ve seftriaksonun (CEFT) sepsis üzerine etkileri denenmiştir. Gereç ve Yöntem: Otuz adet erkek rat Sham, Sepsis, CEFT, OLE-150 ve OLE-300 olmak üzere 5 eşit gruba ayrılarak, model uygulamasından 15 dakika önce 150 mg/kg seftriakson (IP), 150 mg ve 300 mg OLE (PO), uygulandı. Uygulamadan 24 saat sonra kan, akciğer ve kalp dokuları alındı. Serumda kreatin kinaz (CK) ve laktat dehidrojenaz (LDH) düzeyleri ölçülürken, kalp ve akciğer dokularında lipit peroksidasyonu (LPO) ve glutatyon (GSH), süperoksit dismutaz (SOD) ve katalaz (KAT) düzeyleri tespit edildi. Ayrıca, patolojik ve immünohistokimyasal olarak akciğerde interlökin (IL)-8 ve kalpte tümör nekrozis faktör (TNF)-? ekspresyonları belirlendi. Bulgular: Sepsis CK, LDH, LPO, GSH ve kalp CAT aktivitelerinde önemli bir artışa, SOD ve akciğer CAT aktivitesinde ise belirgin azalmaya neden oldu (p<0.001). OLE akciğer ve kalp dokusunda LPO ve GSH seviyelerini anlamlı şekilde düşürmüş (p<0.001), her iki dokuda SOD aktivitesini ve kalp dokusunda CAT aktivitesini arttırmıştır (p<0.001). Ayrıca akciğer dokusunda CEFT LPO seviyesini belirgin şekilde düşürmüş (p<0.01), SOD ve CAT aktivitelerini arttırmıştır (p<0.001). Öneri: OLE’nin antioksidan, IL-8 ve TNF-? seviyelerini düşürerek belirgin anti-inflamatuvar etkileri olduğu belirlenmiştir. Bu nedenle, OLE gibi kuvvetli bir antioksidan sepsis tedavisi için faydalı olabilir.
Aim: Sepsis is a fatal serious clinical disorder. The oxidative stress in sepsis plays an important role in the inflammation. Therefore, the effects of oleuropein (OLE) and ceftriaxone (CEFT) on sepsis were investigated in this study. Materials and Methods: Thirty male rats were divided into five equal groups: Sham, Sepsis, CEFT, OLE-150 and OLE-300, The 150?mg/kg of ceftriaxone (i.p.), and 150 and 300?mg/kg OLE (p.o.) were administered to the treatment groups fifteen minutes before the experiment. After 24 hours, blood samples, lungs and hearts tissues were taken. The creatine kinase (CK) and lactate dehydrogenase (LDH) activities in serum, lipid peroxidation (LPO), glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) levels were all determined. In addition, the expressions of the interleukin (IL)-8 in lung and tumor necrosis factor (TNF)-? in heart were pathologically and immunohistochemically were determined. Results: Sepsis caused significant increases in CK, LDH, LPO, GSH and heart CAT activity and caused inhibition of SOD and lung CAT activity (p<0.001). The OLE significantly decreased the LPO and GSH levels (p<0.001) and increased the SOD in activity of both tissues and CAT activity in lung tissue (p<0.001). Moreover, CEFT significantly decreased the LPO level (p<0.01) and increased tissue SOD and CAT activities in lung tissue (p<0.001). CK and LDH activities in serum showed marked decrease in OLE and CEFT groups (p<0.001). Conclusion: Antioxidant and a notable anti-inflammatory effect of OLE by reducing IL-8 and TNF-? levels have been stated. Thus, a strong antioxidant, such as OLE, would be beneficial for the treatment of sepsis.
Aim: Sepsis is a fatal serious clinical disorder. The oxidative stress in sepsis plays an important role in the inflammation. Therefore, the effects of oleuropein (OLE) and ceftriaxone (CEFT) on sepsis were investigated in this study. Materials and Methods: Thirty male rats were divided into five equal groups: Sham, Sepsis, CEFT, OLE-150 and OLE-300, The 150?mg/kg of ceftriaxone (i.p.), and 150 and 300?mg/kg OLE (p.o.) were administered to the treatment groups fifteen minutes before the experiment. After 24 hours, blood samples, lungs and hearts tissues were taken. The creatine kinase (CK) and lactate dehydrogenase (LDH) activities in serum, lipid peroxidation (LPO), glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) levels were all determined. In addition, the expressions of the interleukin (IL)-8 in lung and tumor necrosis factor (TNF)-? in heart were pathologically and immunohistochemically were determined. Results: Sepsis caused significant increases in CK, LDH, LPO, GSH and heart CAT activity and caused inhibition of SOD and lung CAT activity (p<0.001). The OLE significantly decreased the LPO and GSH levels (p<0.001) and increased the SOD in activity of both tissues and CAT activity in lung tissue (p<0.001). Moreover, CEFT significantly decreased the LPO level (p<0.01) and increased tissue SOD and CAT activities in lung tissue (p<0.001). CK and LDH activities in serum showed marked decrease in OLE and CEFT groups (p<0.001). Conclusion: Antioxidant and a notable anti-inflammatory effect of OLE by reducing IL-8 and TNF-? levels have been stated. Thus, a strong antioxidant, such as OLE, would be beneficial for the treatment of sepsis.
Açıklama
Anahtar Kelimeler
Oleuropein, sepsis modellemesi, seftriakson, yangı, sepsis model, ceftriaxone, inflammation
Kaynak
Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
36
Sayı
3
Künye
Erol, H. S., Aydın, T., Yıldırım, S., Can, İ., Halıcı, M. B., (2020). The effects of oleuropein on lung and heart injury in cecal ligation and puncture - induced sepsis. Eurasian Journal of Veterinary Sciences, 36 (3), 221-231. DOI: 10.15312/EurasianJVetSci.2020.282
