Comparison of cELISA and IFA tests in the serodiagnosis of anaplasmosis in cattle

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dc.contributor.authorEkici, Ozlem Derinbay
dc.contributor.authorSevinc, Ferda
dc.date.accessioned2020-03-26T18:13:58Z
dc.date.available2020-03-26T18:13:58Z
dc.date.issued2011
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThis study was performed to compare indirect fluorescence antibody (IFA) test with competitive ELISA (cELISA) for detection of antibodies to Anaplasma marginale in cattle. In this study, a total of 484 cattle were examined on farms that had a positive history of anaplasmosis. Thin blood smears were prepared from each examined cattle and the cELISA and IFA tests were performed. Samples in which the results of microscopic examination and cELISA were compatible were used as a "gold standard" to define sensitivity and specificity of IFA test and these were used to give "real diagnosis". According to the test result; 62 of 69 samples found positive in "real diagnosis" were positive in IFA test, and all of the 187 samples that were negative in the real diagnosis were defined as negative in IFA test. When compared to gold standard the sensitivity and specificity of the IFA test were 88.95 and 100%, respectively. In conclusion, both IFA and cELISA tests can be used in the diagnosis of A. marginale.en_US
dc.description.sponsorshipSUBAPK (University of Selcuk)Selcuk University [05401028]en_US
dc.description.sponsorshipThe authors would like to acknowledge the financial support from SUBAPK (The Coordination of Scientific Research Projects, University of Selcuk, No. of Project: 05401028), we are indeed grateful.en_US
dc.identifier.endpage1191en_US
dc.identifier.issn1996-0808en_US
dc.identifier.issue10en_US
dc.identifier.startpage1188en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/26252
dc.identifier.volume5en_US
dc.identifier.wosWOS:000293697700009en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherACADEMIC JOURNALSen_US
dc.relation.ispartofAFRICAN JOURNAL OF MICROBIOLOGY RESEARCHen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectAnaplasma marginaleen_US
dc.subjectIFA testen_US
dc.subjectcELISAen_US
dc.titleComparison of cELISA and IFA tests in the serodiagnosis of anaplasmosis in cattleen_US
dc.typeArticleen_US

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