Sjögren sendrom hastaların oral, nazal ve intestinal mikrobiyota profillerinin araştırılması
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Dosyalar
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi, Tıp Fakültesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Sjögren sendromu (SS), başta tükürük ve lakrimal bezler olmak üzere, ekzokrin bezlerinin lenfositik infiltrasyonu ile karakterize kronik otoimmün bağ dokusu hastalığıdır. Son çalışmalar, disbiyozun SS patogenezinde önemli bir rol oynayabileceğini göstermektedir. Ayrıca SS düşük tükürük salgısı oral mikrobiyal homeostazı bozabilir. Bu çalışmada, SS hastalarında kontrollere kıyasla oral, nazal ve intestinal mikrobiata profillerini sağlıklı bireylerle karşılaştırmayı amaçladık. Sjögren sendromu için tüm kriterleri karşılayan 20 hasta ve 20 sağlıklı kontrol araştırmaya dahil edildi. Çalışmaya nazal patolojisi olan, oral kavite hastalıkları olan, malignite öyküsü olan, immün yetmezlik olanlar her iki gruba da dahil edilmedi. Son 3 ay içerisinde antibiyotik kullanımı olanlar her iki grupta da çalışmaya dahil edilmedi. Mikrobiyata, 16S pirodizileme ile analiz edildi. İntestinal mikrobiyom analizinde firmicutes, actinobacteria, bacteriodetes, proteobacteria, clostridia ve prevotella cinsleri arasından bacteriodetes SS grubunda yüksek saptandı (%36,402 karşı %22,821; p<0,05). Nazofarinks mikrobiyom analizinde actinobacteria, firmicutes, ve proteobacteria oranları gruplar arasnda anlamlı farklılık göstermedi. Tükürük mikrobiyom analizinde proteobacteria, firmicutes, bacteriodetes, actinobacteria, neisseria, prevotella, streptococcus ve veillonella cinsleri arasında bacteriodetes SS grubunda yüksek saptandı (%24,7 karşı %16,30; p<0,05). Çalışmamız bu değerli bulgularla, SS hastalarında oral, nazal ve fekal mikrobiyota profilleri inceleyerek bağırsak disbiyozunun yaygın olduğunu gösterdi. Ayrıca tükürük bezlerinin disfonksiyonu, semptomların kötüleşmesi ve hastalığın ciddiyetiyle bağlantılı olan ağız ve bağırsak mikrobiyomunda değişikliklere neden olabileceği bağırsak ve tükürük mikrobiyomlardaki benzerlik ile açıklandı.
Sjögren's syndrome (SS) is a chronic autoimmune connective tissue disease characterized by lymphocytic infiltration of exocrine glands, primarily salivary and lacrimal glands. Recent studies suggest that dysbiosis may play an important role in the pathogenesis of SS. In addition, SS low salivary secretion can impair oral microbial homeostasis. In this study, we aimed to compare oral, nasal and intestinal microbiota profiles in SS patients compared to controls with healthy individuals. Twenty patients and 20 healthy controls who met all criteria for Sjögren's syndrome were included in the study. Patients with nasal pathology, oral cavity diseases, a history of malignancy and immunodeficiency were not included in both groups. Those who used antibiotics in the last 3 months were not included in the study in both groups. The microbiome were analyzed by 16S pyrosequencing. In the microbiome analysis of stools, among the genera firmicutes, actinobacteria, bacteriodetes, proteobacteria, clostridia and prevotella, bacteriodetes were found to be higher in the SS group (36.402% versus 22.821%; p <0.05). In the nasopharynx microbiome analysis, actinobacteria, firmicutes, and proteobacteria ratios did not differ significantly between the groups. In the saliva microbiome analysis, proteobacteria, firmicutes, bacteriodetes, actinobacteria, neisseria, prevotella, streptococcus and veillonella were found to be higher in the bacteriodetes SS group (24.7% vs 16.30%; p <0.05). With These Valuable findings, our study showed that intestinal dysbiosis is common by examining oral, nasal and fecal microbiota profiles in SS patients. The dysfunction of the salivary glands was also explained by the similarity in the gut and salivary microbiomes where it can lead to changes in the oral and gut microbiome which are associated with worsening symptoms and disease severity.
Sjögren's syndrome (SS) is a chronic autoimmune connective tissue disease characterized by lymphocytic infiltration of exocrine glands, primarily salivary and lacrimal glands. Recent studies suggest that dysbiosis may play an important role in the pathogenesis of SS. In addition, SS low salivary secretion can impair oral microbial homeostasis. In this study, we aimed to compare oral, nasal and intestinal microbiota profiles in SS patients compared to controls with healthy individuals. Twenty patients and 20 healthy controls who met all criteria for Sjögren's syndrome were included in the study. Patients with nasal pathology, oral cavity diseases, a history of malignancy and immunodeficiency were not included in both groups. Those who used antibiotics in the last 3 months were not included in the study in both groups. The microbiome were analyzed by 16S pyrosequencing. In the microbiome analysis of stools, among the genera firmicutes, actinobacteria, bacteriodetes, proteobacteria, clostridia and prevotella, bacteriodetes were found to be higher in the SS group (36.402% versus 22.821%; p <0.05). In the nasopharynx microbiome analysis, actinobacteria, firmicutes, and proteobacteria ratios did not differ significantly between the groups. In the saliva microbiome analysis, proteobacteria, firmicutes, bacteriodetes, actinobacteria, neisseria, prevotella, streptococcus and veillonella were found to be higher in the bacteriodetes SS group (24.7% vs 16.30%; p <0.05). With These Valuable findings, our study showed that intestinal dysbiosis is common by examining oral, nasal and fecal microbiota profiles in SS patients. The dysfunction of the salivary glands was also explained by the similarity in the gut and salivary microbiomes where it can lead to changes in the oral and gut microbiome which are associated with worsening symptoms and disease severity.
Açıklama
Anahtar Kelimeler
Sjögren Sendromu, Mikrobiyom, Disbiyoz, Sjögren's Syndrome, Microbiome, Dysbiosis
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Duran, A. (2020). Sjögren sendrom hastaların oral, nazal ve intestinal mikrobiyota profillerinin araştırılması. (Uzmanlık Tezi). Selçuk Üniversitesi, Tıp Fakültesi, Konya.
