Boldenon Uygulamasının Egzersiz Yaptırılan Sıçanlarda Bazı Organ Hasarı Belirteçlerine Etkileri
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Tarih
2023
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi Sağlık Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmanın amacı Boldenon uygulamasının Egzersiz yaptırılan sıçanlarda iskelet kası,
karaciğer ve kalp organlarına etkilerini incelemektir.
Çalışmada 30 günlük 26 adet erkek sıçan (Wistar) kullanıldı. Sıçanlar: Kontrol (K), Egzersiz
(E), Boldenon (B) ve Boldenon + Egzersiz (BE) olmak üzere 4 gruba ayrıldı. K ve E gruplarında 6,
diğer gruplarda ise 7’şer sıçan vardı. E ve BE gruplarındaki sıçanlara 8 hafta boyunca 1.5 km/saat
hızda olacak şekilde haftada 5 gün 45 dakikalık koşu egzersizi yaptırıldı. Buna ek olarak B ve BE
grubundaki sıçanlara haftada 10mg/kg dozda Boldenon uygulaması yapıldı. Deneysel aşama
tamamlandıktan sonra, sıçanların kalplerinden kan alınarak elde edilen serumlardan gerekli
biyokimyasal parametreler ölçüldü. Serum, Alanin transaminaz (ALT), Aspartat Aminotransferaz
(AST), Alkalen Fosfotaz (ALP), Laktat Dehidrogenaz (LDH) ve Kreatin Kinaz (CK) düzeyleri
otoanalizör'de, Cardiyak Troponin-I (cTn-I) ve Kreatin Kinaz Miyokard Bandı (CK-MB) düzeyleri ise
ELİZA kiti ile ölçüldü. Verilerin istatistiki değerlendirilmesinde SPSS 22 paket programı kullanılarak
sonuçlar ort±SS olarak verildi. Verilerin gruplar arası karşılaştırılmalarında ANOVA ve Duncan testi
uygulandı.
8 Haftalık çalışmanın sonunda sıçanlardan alınan kan örneklerinde yapılan istatistiki
inceleme sonucunda AST ve CK-MB değerlerinin B ve BE gruplarında K ve E gruplarına göre
anlamlı ölçüde yüksek çıktığı görülmüştür (p<0,05). ALT ve ALP değerlerinin ise diğer gruplarla
kıyaslandığında E grubunda anlamlı ölçüde düşük çıktığı gözlemlenmiştir (p<0,05). CK ve cTn-I
değerlerinde gruplar arası anlamlı bir farklılık gözlemlenmez iken (p>0,05), her iki parametrede de B
ve BE grubu ortalama değerlerinin K ve E grubu ortalama değerlerinden daha yüksek çıktığı
görülmüştür. LDH değerleri ise E, B ve BE gruplarında K grubuna göre anlamlı ölçüde yüksek
çıkmıştır (p<0,05)
Bulgulardan elde edilen sonuçla; Anabolik-Androjenik Steroid (AAS) kullanımının karaciğer
enzim seviyelerini (ALT, AST, ALP, LDH) ve kalp kası hasarının belirteci olan CK-MB seviyelerini
artırdığı gözlemlenmiştir. Bu bulgularla Anabolik Androjenik Steroid kullanımının kalp ve karaciğer
üzerine olumsuz etkileri olduğu söylenebilir. Ayrıca egzersiz yapmanın AAS’lerin bu olumsuz yan
etkilerinin miktarını azaltmadığı gibi, BE grubunun ortalama değerlerinin tüm parametrelerde diğer
gruplardan daha yüksek çıkması, egzersizin bu yan etkileri daha da artırıyor olabileceğini
gösterebilmektedir.
The aim of this study was to examine the effects of Boldenone administration on skeletal muscle, liver and heart organs in exercised rats. 26 male rats (Wistar) 30 days old were used in the study. Rats were divided into 4 groups as Control (K), Exercise (E), Boldenone (B) and Boldenone + Exercise (BE). There are 6 rats in the K and E groups and 7 rats in the other groups. The rats in groups E and BE were given a 45-minute treadmill exercise 5 days a week at a speed of 1.5km/hour for 8 weeks. In addition, Boldenone was administered to rats in B and BE groups at a dose of 10mg/kg per week. After the experimental phase is completed, the necessary biochemical parameters were measured from the serum obtained by drawing blood from the heart of the rats. Serum, Alanine Transaminase (ALT), Aspartate Aminotransferase (AST), Alkaline Phosphatase (ALP), Lactate Dehydrogenase (LDH), and Creatine Kinase (CK) levels were measured in an autoanalyzer, and Cardiac troponin-I (cTn-I) and Creatine Kinase-Myocardial Band (CK-MB) levels were measured with ELISA kit. In the statistical evaluation of the data, the results were given as mean±SD using the SPSS 22 package program. ANOVA and Duncan tests were used to compare the data between groups. At the end of the 8-week study, blood samples taken from rats were found to be significantly higher in AST and CK-MB values in B and BE groups than in K and E groups as a result of the statistical analysis (p<0,05). It was observed that ALT and ALP values were significantly lower in group E when compared to other groups (p<0,05). While no significant difference was observed between the groups in CK and cTn-I values (p>0,05), it was observed that the mean values of the B and BE groups were higher than the mean values of the K and E groups in both parameters. LDH values were significantly higher in E, B and BE groups compared to K group (p<0.05). With the result obtained from the findings; It has been observed that the use of AAS increases liver enzyme levels (ALT, AST, ALP, LDH) and CK-MB levels, which is a marker of heart muscle damage. With these findings, it can be said that the use of Anabolic Androgenic Steroids has negative effects on the heart and liver. In addition, the fact that the mean values of the BE group were higher than the other groups in all parameters, as exercise did not reduce the amount of these negative side effects of AASs, indicates that exercise may increase these side effects even more.
The aim of this study was to examine the effects of Boldenone administration on skeletal muscle, liver and heart organs in exercised rats. 26 male rats (Wistar) 30 days old were used in the study. Rats were divided into 4 groups as Control (K), Exercise (E), Boldenone (B) and Boldenone + Exercise (BE). There are 6 rats in the K and E groups and 7 rats in the other groups. The rats in groups E and BE were given a 45-minute treadmill exercise 5 days a week at a speed of 1.5km/hour for 8 weeks. In addition, Boldenone was administered to rats in B and BE groups at a dose of 10mg/kg per week. After the experimental phase is completed, the necessary biochemical parameters were measured from the serum obtained by drawing blood from the heart of the rats. Serum, Alanine Transaminase (ALT), Aspartate Aminotransferase (AST), Alkaline Phosphatase (ALP), Lactate Dehydrogenase (LDH), and Creatine Kinase (CK) levels were measured in an autoanalyzer, and Cardiac troponin-I (cTn-I) and Creatine Kinase-Myocardial Band (CK-MB) levels were measured with ELISA kit. In the statistical evaluation of the data, the results were given as mean±SD using the SPSS 22 package program. ANOVA and Duncan tests were used to compare the data between groups. At the end of the 8-week study, blood samples taken from rats were found to be significantly higher in AST and CK-MB values in B and BE groups than in K and E groups as a result of the statistical analysis (p<0,05). It was observed that ALT and ALP values were significantly lower in group E when compared to other groups (p<0,05). While no significant difference was observed between the groups in CK and cTn-I values (p>0,05), it was observed that the mean values of the B and BE groups were higher than the mean values of the K and E groups in both parameters. LDH values were significantly higher in E, B and BE groups compared to K group (p<0.05). With the result obtained from the findings; It has been observed that the use of AAS increases liver enzyme levels (ALT, AST, ALP, LDH) and CK-MB levels, which is a marker of heart muscle damage. With these findings, it can be said that the use of Anabolic Androgenic Steroids has negative effects on the heart and liver. In addition, the fact that the mean values of the BE group were higher than the other groups in all parameters, as exercise did not reduce the amount of these negative side effects of AASs, indicates that exercise may increase these side effects even more.
Açıklama
Anahtar Kelimeler
Anabolik androjenik steroidler, Boldenon, Egzersiz, İskelet kası, Kalp, Karaciğer enzimleri, Anabolic androgenic steroids, Boldenone, Exercise, Heart, Liver enzymes, Skeletal muscle
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Karaduman, B., (2023). Boldenon Uygulamasının Egzersiz Yaptırılan Sıçanlarda Bazı Organ Hasarı Belirteçlerine Etkileri. (Yüksek Lisans Tezi). Selçuk Üniversitesi, Sağlık Bilimleri Enstitüsü, Konya.
