THE EFFECT OF THYMOQUINONE ON THE miRNA PROFILE OF MCF-7 BREAST CANCER CELLS

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dc.contributor.authorSaracligil, Beyza
dc.contributor.authorOzturk, Bahadir
dc.contributor.authorBozkurt, S. Buket
dc.contributor.authorKahveci, Yasemin
dc.date.accessioned2020-03-26T19:42:55Z
dc.date.available2020-03-26T19:42:55Z
dc.date.issued2017
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractBackground and aim: Thymoquinone (TQ), which is the most bioactive component of Nigella sativa (Black cumin), exhibits anticancer characteristics based on cell culture and experimental animal studies. However, molecular action mechanisms of these effects are not clear. MicroRNAs (miRNAs), small non-coding RNAs of approximately 22 nucleotides, are an emerging class of gene expression modulators with relevant roles in several biological processes, including cell differentiation, development, apoptosis, and regulation of the cell cycle. The purpose of this study was to investigate the potential impact of thymoquinone (TQ) on MCF-7 human breast cancer cell miRNAs. Materials and methods: The expression levels of miRNAs in MCF-7 cell and TQ treated MCF-7 cells were estimated by miRNA sequencing. The expressions of miRNAs were determined real-time qPCR. Results: We detected 10 down-regulated mirRNAs (hsa-miR-1, let 7c-5p, hsa-miR-15b-5p, hsa-mir-202-3p, hsa-miR-214-3p, hsa-miR-210-3p, hsa-miR-31-5p, hsa-miR-424-5p, hsa-miR-497-5p, hsa-miR-98-5p) and 2 up regulated miRNAs (hsa-miR-22-3p, hsa-miR-132-3p) in TQ treated groups, comparing with control group. These findings highlight the effects of TQ miRNA profile and molecular mechanism on MCF-7 cells. Conclusion: Finally, according to computational analyses using validated databases PI3 kinase/AKT (hsa04151), Wnt (hsa04310), MAPK (hsa04010) and p53 (hsa 04115) signaling pathways seem to be the key targets of these TQ groups of miRNA.en_US
dc.identifier.doi10.13040/IJPSR.0975-8232.8(7).2849-52en_US
dc.identifier.endpage2852en_US
dc.identifier.issn0975-8232en_US
dc.identifier.issue7en_US
dc.identifier.pmid#YOKen_US
dc.identifier.startpage2849en_US
dc.identifier.urihttps://dx.doi.org/10.13040/IJPSR.0975-8232.8(7).2849-52
dc.identifier.urihttps://hdl.handle.net/20.500.12395/35555
dc.identifier.volume8en_US
dc.identifier.wosWOS:000408879800013en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherINT JOURNAL PHARMACEUTICAL SCIENCES & RESEARCHen_US
dc.relation.ispartofINTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES AND RESEARCHen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectThymoquinoneen_US
dc.subjectmiRNAen_US
dc.subjectBreast canceren_US
dc.titleTHE EFFECT OF THYMOQUINONE ON THE miRNA PROFILE OF MCF-7 BREAST CANCER CELLSen_US
dc.typeArticleen_US

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