Akciğer kanserlerinde NGAL ekspresyonu ve apoptozisle ilişkisi
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Dosyalar
Tarih
2014
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi Sağlık Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada, akciğer kanserlerinde NGAL (nötrofil jelatinaz ilişkili lipokalin= onkojen 24p3 = uterokalin = lipokalin2) expresyonu ve apoptozis ilşkisinin araştırılması amaçlanmıştır. NGAL sentezi neoplazilerde indüklendiğinden, bu proteinin düzeyinden; kanserojenez ve insan tümorlerinin ilerleme safhalarını belirlemek amacıyla faydalanılır. Çalışmaya Selçuk Üniversitesi, Selçuk Tıp Fakültesi Göğüs Cerrahisi Kliniği'ne akciğer kanseri tanısı ile kabul edilen ve cerrahi operasyon geçiren 40-70 yaşları arasındaki hastalar dahil edilmiştir. Akut inme, romatolojik hastalıklar, kronik böbrek hastalığı, konjestif kalp yetmezliği, kronik enfeksiyon ve akciğer kanseri dışında diğer organ ve sistem kanserlerine sahip hastalar çalışma dışı bırakılmıştır. ELISA yöntemiyle hastaların serum NGAL ve serum M30 düzeylerinin değerlendirildiği aşamada, Akciğer kanseri (N=40) ve Normal grup (N=40) olmak üzere 2 grup oluşturulmuştur. Yapılan analizlerde, serum NGAL düzeyleri; akciğer kanseri grubunda ve normal grupta sırasıyla 424,03±74,49 ve 374,04± 90,34 ng/L olarak bulunmuştur. Normal gruba göre akciğer kanseri grubundaki bu artış istatistiki açıdan anlamlı olarak değerlendirilmiştir (p<0.01). Dolaşımdaki apoptozis belirteci olan M30 düzeyleri ise; akciğer kanseri grubunda ve normal grupta sırasıyla 144,08±45,92 ve 118,76± 46,16 U/L olarak bulunmuştur. Normal gruba göre akciğer kanseri grubundaki bu artış istatistiki açıdan anlamlı olarak değerlendirilmiştir (p<0.01). Kanserli ve kanserli dokuya komşu normal doku örneklerindeki kaspaz 3, kaspaz 8 ve kaspaz 9 antijen düzeyleri de immünohistokimyasal boyama yöntemleriyle belirlenerek apoptotik indeksler hesaplanmıştır. Kanserli dokularda apoptotik indeks,normal dokulardakine göre istatistiksel fark oluşacak şekilde (p<0,01) daha az belirlenmiştir. Bu durum, akciğer kanserinde baskın hücre ölüm tipinin apoptoz dışındaki diğer hücre ölüm yollarıyla olduğunu göstermektedir. Ayrıca kanserli dokularda kanser hücrelerinin apoptozisi engellediği görüşü bu sonuçlarla desteklenmektedir. Sonuç olarak, çeşitli faktörlere bağlı olarak oluşan akciğer kanserlerinde NGAL düzeylerinin, kanser hücrelerinde farklı mekanizmalarla devre dışı kalan ve kanser hücrelerinin daha uzun süre yaşamasına yol açan apoptotik süreçle ilişkili olduğu öngörülmektedir.
The Expression of NGAL in Lung Cancers and Its Relation with Apoptosis In this study, it was aimed that to investigate expression of NGAL (neutrophil gelatinase associated lipocalin = oncogenic 24p3 =uterokal = lipokalin2) in lung cancers and its relation with apoptosis. The NGAL synthesis induce in neoplasms, therefore, it is benefited from the level of this protein for determination of carcinogenesis and the progress stages of human tumors. For this study, patients between the ages of 40-70 accepted by thoracic Surgery Clinic (Selçuk University, Faculty of Medicine) with the diagnosis of lung cancer and they would be surgery operation were involved. Patients having acute stroke, rheumatic diseases, chronic kidney disease, congestive heart failure, chronic infection, other organ and system cancer (except lung cancer) were excluded from the study. Two group including lung cancer (N = 40) and normal group (N = 40) were formed to determine levels of NGAL and M30 in patient serums by using ELISA method. According to the analysis results, serum NGAL levels for the lung cancer group and the normal group were found as 424,03±74,49 and 374,04± 90,34 ng/L, respectively. This increase in lung cancer group was found statistically significant according to normal group (p<0,01). M30 levels which is apoptosis marker in curcilation for the lung cancer group and the normal group were obtained as 144,08±45,92 and 118,76± 46,16 U/L, respectively. This increase in lung cancer group was found statistically significant according to normal group (p<0,01). In samples of cancerous tissues and normal tissues adjacent to the cancerous tissue, caspase 3, caspase 8 and caspase 9 antigen levels were determined by immunohistochemical staining methods, and apoptotic indexes were calculated. It was obtained that apoptotic indexes in cancerous tissues were lower than normal tissues. This decrease was found significantly (p<0,01). This result show that the predominant type of cell death in lung cancer was other cell death pathways except apoptosis pathway. In adition, these results support to the opinion which is apoptosis in cancerous tissues may be inhibited by cancer cells. Consequently, we are expected that NGAL levels in lung cancers which has depend on various factors are associate for apoptosis which is called programmed cell death, remaining disabled through different mechanisms in cancer cells and leading to longer life of cancer cells.
The Expression of NGAL in Lung Cancers and Its Relation with Apoptosis In this study, it was aimed that to investigate expression of NGAL (neutrophil gelatinase associated lipocalin = oncogenic 24p3 =uterokal = lipokalin2) in lung cancers and its relation with apoptosis. The NGAL synthesis induce in neoplasms, therefore, it is benefited from the level of this protein for determination of carcinogenesis and the progress stages of human tumors. For this study, patients between the ages of 40-70 accepted by thoracic Surgery Clinic (Selçuk University, Faculty of Medicine) with the diagnosis of lung cancer and they would be surgery operation were involved. Patients having acute stroke, rheumatic diseases, chronic kidney disease, congestive heart failure, chronic infection, other organ and system cancer (except lung cancer) were excluded from the study. Two group including lung cancer (N = 40) and normal group (N = 40) were formed to determine levels of NGAL and M30 in patient serums by using ELISA method. According to the analysis results, serum NGAL levels for the lung cancer group and the normal group were found as 424,03±74,49 and 374,04± 90,34 ng/L, respectively. This increase in lung cancer group was found statistically significant according to normal group (p<0,01). M30 levels which is apoptosis marker in curcilation for the lung cancer group and the normal group were obtained as 144,08±45,92 and 118,76± 46,16 U/L, respectively. This increase in lung cancer group was found statistically significant according to normal group (p<0,01). In samples of cancerous tissues and normal tissues adjacent to the cancerous tissue, caspase 3, caspase 8 and caspase 9 antigen levels were determined by immunohistochemical staining methods, and apoptotic indexes were calculated. It was obtained that apoptotic indexes in cancerous tissues were lower than normal tissues. This decrease was found significantly (p<0,01). This result show that the predominant type of cell death in lung cancer was other cell death pathways except apoptosis pathway. In adition, these results support to the opinion which is apoptosis in cancerous tissues may be inhibited by cancer cells. Consequently, we are expected that NGAL levels in lung cancers which has depend on various factors are associate for apoptosis which is called programmed cell death, remaining disabled through different mechanisms in cancer cells and leading to longer life of cancer cells.
Açıklama
Anahtar Kelimeler
Akciğer kanseri, NGAL, Apoptozis, M30, Kaspaz, Lung cancer, Apoptosis, Caspase
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Çiftçi, H. (2014). Akciğer kanserlerinde NGAL ekspresyonu ve apoptozisle ilişkisi. Selçuk Üniversitesi, Yayımlanmış doktora tezi, Konya.
