Supplementation of dairy cows with bovine somatotropin or omega-3 rich fish oil affects the endometrial expression of peroxisome proliferator-activated receptors
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Dosyalar
Tarih
2011
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi Veterinerlik Fakültesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Çalışmanın amaçları, holştayn ırkı ineklerin rasyonuna 90 gün boyunca zenginleştirilmiş balık yağı formunda uzun zincirli omega-3 çoklu doymamış balık yağı katılması- nın veya ovulasyon günü ve 11 gün sonrasında iki kere inek somatotropin enjeksiyonunun endometriumunda PPAR ekspresyonu ve aktivasyonunu araştırmaktır. Gereç ve Yöntem: Laktasyonda olmayan inekler siklik, siklik-bST, gebe veya gebe-bST olmak üzere 4 gruba ayrıldı. Laktasyondaki inekler ise siklik, siklik-bST, gebe, gebe-bST, siklik-FO veya siklik-FO-bST olmak üzere 6 gruba ayrıldı. Bulgular: Northern ve Western blot analizleri PPAR? ve PPAR?’nın ovulasyon sonrası 17. günde endometriumda ekspre edildiğini belirlendi ancak PPAR? tespit edilemedi. bST uygulaması sadece gebelerde PPAR? mRNA ekspresyonunu arttırdı, bu etki embriyo tarafından düzenlenmiş olabilir. Laktasyondaki gebe ineklerin endometriumundaki bST’ya bağlı PPAR? mRNA miktarındaki artış bST etkisinin gebelik durumuna bağlı olduğunu vurgulamaktadır. Rasyona balık yağı eklenmesi PPAR? mRNA miktarını azaltırken, PPAR? mRNA miktarı üzerine bir etkisi olmadı. PPAR? proteini luminal epitelde, glandular epitelde, subepitel stromada ve az miktarda da adluminal stromada belirlendi. Anti-PPAR? reaksiyonu bST uygulaması ve balık yağı beslemesine bağlı olarak gebe ineklerde azaldı. Öneri: bST uygulaması ve balık yağı beslemesi endometrial PPAR? ve PPAR? ekspresyonun laktasyondaki sütçü ineklerde etkilemektedir.
Aim: The study objectives were to determine whether dietary supplementation with long chain omega-3 polyunsaturated fatty acids in the form of enriched fish oil (FO) for 90 days or treatment with bovine somatotropin (bST) at the time of ovulation and 11 days post-ovulation influenced PPAR expression and activation in bovine endometrium in Holstein cows. Materials and Methods: Non-lactating cows were assigned to one of four treatments: cyclic, cyclic-bST, pregnant or pregnant-bST. Lactating cows were assigned to one of six treatments: cyclic, cyclic-bST, pregnant, pregnant-bST, cyclic-FO or cyclic-FO-bST. Results: Northern and Western blot analyses indicated that PPAR? and PPAR?, but not PPAR?, are expressed in endometrium from all cows at day 17 post-ovulation. Treatment with bST is associated with increased PPAR? mRNA abundance in pregnant but not cyclic cows, suggesting that the effect may be mediated by the embryo. Increased abundances of PPAR? mRNA are observed in response to bST during pregnancy in lactating cows but not in non-lactating cows, highlighting the importance of lactation status in determining bST response. Fish oil supplementation is associated with reduced PPAR? mRNA abundance, but did not affect steady-state PPAR? mRNA abundance. PPAR? protein is expressed in the luminal epithelium, glandular epithelium, subepithelial stroma and to a lesser extent in the adluminal stroma. Anti-PPAR? reactivity is reduced in response to bST and fish oil treatments in pregnant cows. Conclusion: bST and fish oil treatments affect endometrial PPAR? and PPAR? expression in lactating dairy cows.
Aim: The study objectives were to determine whether dietary supplementation with long chain omega-3 polyunsaturated fatty acids in the form of enriched fish oil (FO) for 90 days or treatment with bovine somatotropin (bST) at the time of ovulation and 11 days post-ovulation influenced PPAR expression and activation in bovine endometrium in Holstein cows. Materials and Methods: Non-lactating cows were assigned to one of four treatments: cyclic, cyclic-bST, pregnant or pregnant-bST. Lactating cows were assigned to one of six treatments: cyclic, cyclic-bST, pregnant, pregnant-bST, cyclic-FO or cyclic-FO-bST. Results: Northern and Western blot analyses indicated that PPAR? and PPAR?, but not PPAR?, are expressed in endometrium from all cows at day 17 post-ovulation. Treatment with bST is associated with increased PPAR? mRNA abundance in pregnant but not cyclic cows, suggesting that the effect may be mediated by the embryo. Increased abundances of PPAR? mRNA are observed in response to bST during pregnancy in lactating cows but not in non-lactating cows, highlighting the importance of lactation status in determining bST response. Fish oil supplementation is associated with reduced PPAR? mRNA abundance, but did not affect steady-state PPAR? mRNA abundance. PPAR? protein is expressed in the luminal epithelium, glandular epithelium, subepithelial stroma and to a lesser extent in the adluminal stroma. Anti-PPAR? reactivity is reduced in response to bST and fish oil treatments in pregnant cows. Conclusion: bST and fish oil treatments affect endometrial PPAR? and PPAR? expression in lactating dairy cows.
Açıklama
Anahtar Kelimeler
İnek, endometrium, PPAR, bST, balık yağı, Cow, fish oil
Kaynak
Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
27
Sayı
4
Künye
MacLaren, L. A., Bilby, T. R., Michel, F., Guzeloglu, A., Staples, C. R., Thatcher, W. W., (2011). Supplementation of dairy cows with bovine somatotropin or omega-3 rich fish oil affects the endometrial expression of peroxisome proliferator-activated receptors. Eurasian Journal of Veterinary Sciences, 27 (4), 207-218.
