Investigation of Nosema Apis and Nosema Ceranae in Bees by Multiplex PCR in Kırıkkale Region
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Tarih
2023 Aralık
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışmanın amacı, Kırıkkale yöresindeki bal arılarında Nosemosis varlığının ve yaygınlığının Multiplex PCR ile belirlenmesidir. Gereç ve Yöntem: Kırıkkale İl Merkezi, Bahşili, Balışeyh, Keskin, Sulakyurt ve Yahşihan ilçelerinde bulunan 52 adet arılıktan numuneler alınmıştır. Her 5 koloniden toplanan 10 arının göğüs ve karın kısımları diseke edilerek porselen havanda steril fizyolojik su ile ezildi. Nosema sporları içeren numuneler Safranin ile boyandı ve aynı numunelere tür teşhisi için multipleks PCR uygulandı. Bulgular: Nosema spp. sporları, 52 arılığın 12'sinde (%23,07) mikroskobik olarak gözlenmiştir. Pozitif örnekler arasında en yüksek oran %57,14 ile Balışeyh ilçesinde bulundu. Balışeyh ilçesini %33,33 ile Delice ilçesi ve %23,07 ile Kırıkkale/Merkez ilçesi izlemektedir. Bahşili, Keskin, Sulakyurt ve Yahşihan ilçelerinden alınan örneklerde Nosema sporlarına rastlanmadı. Genel olarak ilçelerin enfeksiyon oranları Merkez (%5,76), Balışeyh (%7,69), Delice (%9,61) olarak belirlendi. Tüm pozitif örnekler multipleks PCR ile N. ceranae olarak tespit edildi. Öneri: Bu Kırıkkale yöresi bal arılarında Nosemosis varlığı, yaygınlığı ve etiyolojik ajanı olan N. ceranae'nin belirlendiği ilk çalışmadır. Bu çalışmada elde edilen veriler doğrultusunda bölgedeki arı kayıplarında Nosemosisin mutlaka göz önünde bulundurulması ve arıcıların konu hakkında bilinçlendirilmesi gerektiği kanaatine varılmıştır.
Aim: The aim of this study was conducted to determine the presence and prevalence of Nosemosis in honeybees in Kırıkkale region by Multiplex PCR. Materials and Methods: Samples were collected from 52 apiaries in Kırıkkale City Center, Bahşili, Balışeyh, Keskin, Sulakyurt and Yahşihan districts. The thorax and abdomen of ten bees collected from every 5 colonies were dissected and crushed with sterile physiological water in a porcelain mortar. The samples with Nosema spores were stained with Saffron and the multiplex PCR was applied to detect specimens to the same samples. Results: Nosema spp. spores were observed in 12 out of 52 apiaries (23.07%) microscopically. Among the positive samples, Balışeyh county was found with the highest rate of 57.14%. The township district is followed by Delice district with 33.33% and Kırıkkale /Center district with a ratio of 23.07%. Nosema spores were not found in samples taken from Bahşili, Keskin, Sulakyurt and Yahşihan districts. In general, the infection rates of the districts were determined as Central (5.76%), Balışeyh (7.69%), and Delice (9.61%). All the positive samples were detected as N. ceranae by multiplex PCR. Conclusion: This is the first study to determine the presence and prevalence of Nosemosis in honey bees in the Kırıkkale region and its etiological agent, N. ceranae. In line with the data obtained in this study, it was concluded that Nosemosis should be taken into consideration in bee losses in the region and beekeepers should be made aware of the issue.
Aim: The aim of this study was conducted to determine the presence and prevalence of Nosemosis in honeybees in Kırıkkale region by Multiplex PCR. Materials and Methods: Samples were collected from 52 apiaries in Kırıkkale City Center, Bahşili, Balışeyh, Keskin, Sulakyurt and Yahşihan districts. The thorax and abdomen of ten bees collected from every 5 colonies were dissected and crushed with sterile physiological water in a porcelain mortar. The samples with Nosema spores were stained with Saffron and the multiplex PCR was applied to detect specimens to the same samples. Results: Nosema spp. spores were observed in 12 out of 52 apiaries (23.07%) microscopically. Among the positive samples, Balışeyh county was found with the highest rate of 57.14%. The township district is followed by Delice district with 33.33% and Kırıkkale /Center district with a ratio of 23.07%. Nosema spores were not found in samples taken from Bahşili, Keskin, Sulakyurt and Yahşihan districts. In general, the infection rates of the districts were determined as Central (5.76%), Balışeyh (7.69%), and Delice (9.61%). All the positive samples were detected as N. ceranae by multiplex PCR. Conclusion: This is the first study to determine the presence and prevalence of Nosemosis in honey bees in the Kırıkkale region and its etiological agent, N. ceranae. In line with the data obtained in this study, it was concluded that Nosemosis should be taken into consideration in bee losses in the region and beekeepers should be made aware of the issue.
Açıklama
Anahtar Kelimeler
Apis mellifera, Multiplex PCR, N. apis, N. ceranae, Kırıkkale
Kaynak
Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
39
Sayı
4
Künye
Aydenizöz, M., Yorulmaz, A. C., (2023). Investigation of Nosema Apis and Nosema Ceranae in Bees by Multiplex PCR in Kırıkkale Region. Eurasian Journal of Veterinary Sciences, 39(4), 171-176.
DOI: 10.15312/EurasianJVetSci.2023.414
