Production of fatty-acyl-glutamate biosurfactant by Bacillus subtilis on soybean co-products

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dc.contributor.authorMarti, Mustafa E.
dc.contributor.authorColonna, William J.
dc.contributor.authorReznik, Gabriel
dc.contributor.authorPynn, Michelle
dc.contributor.authorJarrell, Kevin
dc.contributor.authorLamsal, Buddhi
dc.contributor.authorGlatz, Charles E.
dc.date.accessioned2020-03-26T19:06:46Z
dc.date.available2020-03-26T19:06:46Z
dc.date.issued2015
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractFatty-acyl-glutamate (FA-Glu), a surfactin variant has been successfully produced using a genetically modified strain of Bacillus subtilis grown on glucose. However, yields with soybean hulls (SBH) replacing glucose were lower. This work was undertaken to reduce the yield loss when using SBH as the carbon source and to evaluate two other soy by-products, namely fiber and skim from aqueous oil extraction as alternative carbon and nitrogen sources. Fermentation of soybean hulls, fibers and skim at various concentrations produced lower FA-Glu titers compared to S-7 medium. Neither increasing their amount nor supplementing with glucose increased the FA-Glu titer, suggesting the presence of an inhibitor in these feedstocks. By using a mixture of polysaccharide-degrading enzymes, over 65% of SBH solids were converted to soluble carbohydrates. FA-Glu titers obtained from SBH hydrolysates containing residual hull solids were still low; however, with the removal of the solids, cell growth improved and FA-Glu yield was 60% higher than with glucose. Thus, this low-cost material can be converted to a substrate for production of FA-Glu biosurfactant. Unmodified fiber and skim components of aqueous oil extraction were not beneficial. (C) 2014 Elsevier BAT. All rights reserved.en_US
dc.description.sponsorshipUSDA for the CREES [2010-34432-20955]; United Soybean Board for the USB [2488]en_US
dc.description.sponsorshipThe authors acknowledge USDA for the CREES Grant # 2010-34432-20955 and United Soybean Board for the USB Award #2488. The authors thank Dr. Kamel Harrata of the Iowa State University Chemical Instrumentation Facility for his expertise in running FA-Glu analyses.en_US
dc.identifier.doi10.1016/j.bej.2014.11.011en_US
dc.identifier.endpage55en_US
dc.identifier.issn1369-703Xen_US
dc.identifier.issn1873-295Xen_US
dc.identifier.startpage48en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.bej.2014.11.011
dc.identifier.urihttps://hdl.handle.net/20.500.12395/32467
dc.identifier.volume95en_US
dc.identifier.wosWOS:000349428000005en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.relation.ispartofBIOCHEMICAL ENGINEERING JOURNALen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectFA-Gluen_US
dc.subjectBiosurfactanten_US
dc.subjectSoybean hullen_US
dc.subjectEnzyme Technologyen_US
dc.subjectFermentationen_US
dc.subjectSubstrate Inhibitionen_US
dc.titleProduction of fatty-acyl-glutamate biosurfactant by Bacillus subtilis on soybean co-productsen_US
dc.typeArticleen_US

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