Investigation of Fe(III) bonding properties of tetracaine using spectrofluorimetric method
Küçük Resim Yok
Tarih
2015
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The metal bonding properties of tetracaine (TCN) were investigated by using spectrofluorimetric method. Fluorescence quenching of TCN by Fe(III) was studied and optimal experimental conditions were determined. The excitation and emission wavelengths were lex= 306 nm and lem= 373 nm for the Fe(III)-TCN complex in water medium. The calibration curve was obtained from Stern-Volmer equation plotting to F0/F-[Fe3+] graphs and the linearity was found within the range of 0.22-2.23mg.ml-1 for the Fe(III)-TCN complex under optimal conditions. The detection limit (LOD) and the quantitation limits (LOQ) of the proposed method for this complex were 8.29 ng.ml-1 and 27.60 ng.ml-1 respectively. The stochiometry of this complex was also determined by using the continuous variation method and the molar ratio of Fe(III)-TCN was calculated as 1:1. The interaction between the tetracaine in eye drop form and standard Fe(III) was investigated by applying this quenching method and obtained satisfactory results.
Açıklama
Anahtar Kelimeler
Fe(III) complex, fluorescence quenching, pharmaceutical analysis, tetracaine
Kaynak
Journal of Selcuk University Natural and Applied Science
WoS Q Değeri
Scopus Q Değeri
Cilt
4
Sayı
1
Künye
Gunduz, S. B., Muslumoglu, M. (2015). Investigation of Fe(III) bonding properties of tetracaine using spectrofluorimetric method. Journal of Selcuk University Natural and Applied Science, 4, (1), 27-40.
