Prevalence of Eimeria species in sheep in Eskişehir province
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Dosyalar
Tarih
2020
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Çalışma koyun yetiştiriciliğinin yaygın olarak yapıldığı Eskişehir ilinde, koyunlarda bulunan Eimeria türlerinin yaygınlığını araştırmak amacıyla yapılmıştır. Gereç ve Yöntem: Araştırma Eskişehir iline bağlı Seyitgazi, İnönü, Mihalgazi ile Mahmudiye ilçelerinde yetiştirilen 300 merinos ırkı koyun üzerinde yürütülmüştür. Her bir hayvanın rektumundan tekniğine uygun olarak alınan dışkı numuneleri Fulleborn’un doymuş tuzlu su metodu ile incelenmiştir. Pozitif çıkan dışkı numunelerinde gram dışkıdaki Eimeria ookist miktarı (OPG) Modifiye McMaster yöntemi ile belirlenmiştir. Daha sonra, her bir pozitif örnek ayrı petri kutularında üzerine %2,5'luk Potasyum dikromat ilave edildikten sonra laboratuvarda sporlanmaya bırakılmıştır. Sporlanmış ookistlerin ölçümü Nikon Eclipse 80i trinoküler araştırma mikroskobunun x100'lük büyütmesinde DS-5M-L1 dijital kamera sistemi ile mikrometrik olarak yapıldıktan sonra tür teşhisleri ilgili kaynaklardan yararlanılarak yapılmıştır. Araştırmada elde edilen verilerin istatistiksel değerlendirmesinde Ki-Kare (x²) testi kullanılmıştır. Bulgular: Araştırma sonucunda muayene edilen 300 adet dışkının 61 (%20 .33)'inde Eimeria ookistlerine rastlanmıştır. Eimeria ovinoidalis (%57,38), E. ahsata (%45,90), E. bakuensis (%42.62), E. parva (%42.62), E. crandallis (%11.48), E. granulosa (%4.92), E. faurei (%3.28), E. intricata (%3.28) ve E. weybridgensis (%3.28) olmak üzere dokuz Eimeria türü teşhis edilmiştir. Enfeksiyon oranı genç hayvanlarda, yaşlı hayvanlardan daha yüksek bulunmuştur (p<0.05). Enfekte hayvanlarda 2-5 türle enfeksiyon yaygın olarak gözlenmiştir. Muayene edilen hayvanlarda klinik bulgulara rastlanmamıştır. Öneri: Bu çalışmada, patojen türlerin yaygın olarak bulunması Koksidiosis’in Eskişehir ili koyunlarında risk oluşturabileceği, hastalıktan korunma ve kontrol yöntemleri konusunda yetiştiricilerin bilinçlendirilmesi ile hastalığın neden olacağı ekonomik kayıpların azaltılabileceği kanaatine varılmıştır.
Aim: The study was carried out to investigate the occurrence of Eimeria species in sheep in Eskişehir province, Turkey, where sheep breeding is common. Materials and Methods: This study was carried out on 300 merino sheep raised in the Seyitgazi, İnönü, Mihalgazi and Mahmudiye districts of Eskişehir. Fecal samples from each sheep were brought to the laboratory and examined by Fulleborn saturated salt flotation method. Eimeria oocyst number per gram of feces (OPG) was determined by the Modified McMaster method in positive fecal samples. Later, each positive sample was added to 2.5% Potassium dichromate in separate Petri dishes and left to be sporulated in the laboratory. The measurement of sporulated oocysts was made micro metrically with the DS-5M-L1 digital camera system at x100 magnification of the Nikon Eclipse 80i trinocular research microscope. Species identification of Eimeria oocysts was made according to the morphological features reported in the literature. Chi-square (x²) test was used to analyze the data. Results: Eimeria species were identified in 61 (20.33%) of the 300 samples of feces. In total nine different Eimeria species were identified including Eimeria ovinoidalis (57.38%), E. ahsata (45.90%), E. bakuensis (42.62%), E. parva (42.62%), E. crandallis (11.48%), E. granulosa (4.92%), E. faurei (3.28%), E. intricata (3.28%) and E. weybridgensis (3.28%). The infection rate was higher in young animals than older animals (p <0.05). Infection with 2-5 species was frequently detected in infected animals. No clinical findings were observed in the examined animals. Conclusion: The high prevalence of pathogenic species shows that Coccidiosis is a risk for sheep raised in Eskişehir province. Potential economic effects of Coccidiosis can be reduced by informing breeders about disease prevention and control methods.
Aim: The study was carried out to investigate the occurrence of Eimeria species in sheep in Eskişehir province, Turkey, where sheep breeding is common. Materials and Methods: This study was carried out on 300 merino sheep raised in the Seyitgazi, İnönü, Mihalgazi and Mahmudiye districts of Eskişehir. Fecal samples from each sheep were brought to the laboratory and examined by Fulleborn saturated salt flotation method. Eimeria oocyst number per gram of feces (OPG) was determined by the Modified McMaster method in positive fecal samples. Later, each positive sample was added to 2.5% Potassium dichromate in separate Petri dishes and left to be sporulated in the laboratory. The measurement of sporulated oocysts was made micro metrically with the DS-5M-L1 digital camera system at x100 magnification of the Nikon Eclipse 80i trinocular research microscope. Species identification of Eimeria oocysts was made according to the morphological features reported in the literature. Chi-square (x²) test was used to analyze the data. Results: Eimeria species were identified in 61 (20.33%) of the 300 samples of feces. In total nine different Eimeria species were identified including Eimeria ovinoidalis (57.38%), E. ahsata (45.90%), E. bakuensis (42.62%), E. parva (42.62%), E. crandallis (11.48%), E. granulosa (4.92%), E. faurei (3.28%), E. intricata (3.28%) and E. weybridgensis (3.28%). The infection rate was higher in young animals than older animals (p <0.05). Infection with 2-5 species was frequently detected in infected animals. No clinical findings were observed in the examined animals. Conclusion: The high prevalence of pathogenic species shows that Coccidiosis is a risk for sheep raised in Eskişehir province. Potential economic effects of Coccidiosis can be reduced by informing breeders about disease prevention and control methods.
Açıklama
Anahtar Kelimeler
Coccidiosis, Eimeria, koyun, Eskişehir, sheep
Kaynak
Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
36
Sayı
4
Künye
Eser, M., Göksu, A., Çiçek, H., Erez, M. S., Çiçek, H., (2020). Prevalence of Eimeria species in sheep in Eskişehir province. Eurasian Journal of Veterinary Sciences, 36 (4), 261-266. DOI: 10.15312/EurasianJVetSci.2020.307
