Presence and antimicrobial resistance profiles of Salmonella spp. in retailed sausages in Kayseri, Turkey
Küçük Resim Yok
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çal ı ş mada Kayseride satı ş a sunulan sucuklarda Salmonella spp.nin varl ı ğ ı ve elde edilen izolatlarda antimikrobiyel duyarl ı l ı ğ ı n ı n ara ş tı rı lmas ı amaçland ı . Toplam 100 numune incelendi. Salmonella spp. izolasyonu için ISO 6579 sayı l ı standard ı nda bildirilen kültür tekni ğ i modifiye edilerek kullan ı ld ı ve izolatlar PCR ile do ğ ruland ı . İzolatları n ampisilin, enrofloksasin, sefazolin, danofloksasin, gentamisin, nalidiksik asit, neomisin, oksitetrasiklin ve trimetoprim-sülfametaksazol antibiyotiklerine duyarl ı l ı kları disk difuzyon metodu ile belirlendi. İncelenen 100 örne ğ in 4ünden (%4) Salmonella spp. izole edildi. Bütün izolatlar sadece neomisine dirençli ve test edilen di ğ er antibiyotiklerden gentamisin haricindeki antibiyotiklere duyarl ı olarak belirlendi. Sadece bir adet Salmonella spp. izolatı gentamisine orta derecede duyarl ı bulundu. Elde edilen sonuçlar sucukları n Salmonella spp. ile sporadik olarak kontamine olabilece ğ ini ve halk sa ğl ı ğ ı için risk olu ş turabilece ğ ini göstermektedir.
This study was carried out to investigate the presence and antimicrobial susceptibilities of Salmonella spp. isolates from sausage obtained from retail outlets in Kayseri, Turkey. A total of 100 samples were analyzed in the study. The method proposed by ISO 6579 with minor modifications, for isolation of Salmonella spp. was used and the isolates were confirmed by PCR. Antibacterial susceptibility testing of the isolates to ampicillin, enrofloxacin, cefazolin, danofloxacin, gentamicin, nalidixic acid, neomycine, oxytetracycline and trimethoprim-sulfamethoxazol was performed by disc diffusion method. Salmonella spp. was isolated from 4 (4%) out of 100 samples tested. All isolates, were resistant only to neomycine and were susceptible to other antimicrobial agents except for gentamicin. Only one Salmonella isolate was found to be susceptible (intermediate level) to gentamicin. These results demonstrate that sausage samples may be sporadically contaminated with Salmonella spp. and therefore present a potential risk for public health.
This study was carried out to investigate the presence and antimicrobial susceptibilities of Salmonella spp. isolates from sausage obtained from retail outlets in Kayseri, Turkey. A total of 100 samples were analyzed in the study. The method proposed by ISO 6579 with minor modifications, for isolation of Salmonella spp. was used and the isolates were confirmed by PCR. Antibacterial susceptibility testing of the isolates to ampicillin, enrofloxacin, cefazolin, danofloxacin, gentamicin, nalidixic acid, neomycine, oxytetracycline and trimethoprim-sulfamethoxazol was performed by disc diffusion method. Salmonella spp. was isolated from 4 (4%) out of 100 samples tested. All isolates, were resistant only to neomycine and were susceptible to other antimicrobial agents except for gentamicin. Only one Salmonella isolate was found to be susceptible (intermediate level) to gentamicin. These results demonstrate that sausage samples may be sporadically contaminated with Salmonella spp. and therefore present a potential risk for public health.
Açıklama
Anahtar Kelimeler
Veterinerlik
Kaynak
Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
28
Sayı
1
