A Highly Efficient Automated Flow Injection Method for Rapid Determination of Free Fatty Acid Content in Corn Oils

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dc.contributor.authorAyyildiz, H. Filiz
dc.contributor.authorKara, Huseyin
dc.date.accessioned2020-03-26T18:49:10Z
dc.date.available2020-03-26T18:49:10Z
dc.date.issued2014
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIn the present study, a new flow injection (FI) methodology for determination of free fatty acid (FFA) content in corn oil samples is proposed. The proposed method is based on monitoring the absorbance changes at 580 nm, lambda (max), as a result of neutralization of FFA in oil samples by KOH in the reagent. The analyses were performed with a single-line FI manifold system created by modification of high performance liquid chromatography. The main parameters, such as sample and reagent volumes, reaction coil, reagent concentration and temperature were all optimized. With the newly developed sampling strategy, the oil sample and reagent at micro level were directly injected together without any pre-treatment. The proposed flow injection analysis method was validated statistically and it was found to be linear (between 0.09 and 1.50 FFA %), accurate (recovery 87.19-122.22 %), and precise (relative standard deviation < 1 % for both intra-day and inter-day precision). The limit of detection and limit of quantification were found to be 7.41 x 10(-3) and 2.24 x 10(-2) oleic acid %, respectively. The results were also compared with those obtained by the American Oil Chemists Society (Ca-5a-40) method using statistical t and F tests, and a significant difference was not observed between the methods at the 95 % confidence level. These results strongly suggest that this method is suitable for automated routine analysis of FFA in edible oils due to its simplicity, reliability, speed, and economy of solvents and sample.en_US
dc.description.sponsorshipSelcuk University Coordinators of Scientific ResearchSelcuk University [SU-BAP 08-101029]en_US
dc.description.sponsorshipThis study, a part of Ph.D. work entitled "Improvement of Edible Oil Analysis Methods by Flow Injection Systems'' was supported by Selcuk University Coordinators of Scientific Research with SU-BAP 08-101029 project numbers. The authors wish to thank the principal of Selcuk University and Scientific Research Projects Coordination.en_US
dc.identifier.doi10.1007/s11746-013-2403-0en_US
dc.identifier.endpage558en_US
dc.identifier.issn0003-021Xen_US
dc.identifier.issn1558-9331en_US
dc.identifier.issue4en_US
dc.identifier.startpage549en_US
dc.identifier.urihttps://dx.doi.org/10.1007/s11746-013-2403-0
dc.identifier.urihttps://hdl.handle.net/20.500.12395/30543
dc.identifier.volume91en_US
dc.identifier.wosWOS:000333427000003en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherSPRINGERen_US
dc.relation.ispartofJOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectAutomated flow injection analysis (FIA)en_US
dc.subjectFree fatty acid (FFA)en_US
dc.subjectCorn oilen_US
dc.subjectNew sampling strategyen_US
dc.subjectMethod validationen_US
dc.titleA Highly Efficient Automated Flow Injection Method for Rapid Determination of Free Fatty Acid Content in Corn Oilsen_US
dc.typeArticleen_US

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