Endometrial hiperplaziler ve endometrium kanserinde toll-lıke reseptör ekspresyonunun klinikopatolojik değişkenler ile değerlendirilmesi
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Tarih
2013
Yazarlar
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Cilt Başlığı
Yayıncı
Selçuk Üniversitesi Tıp Fakültesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Toll-like reseptör (TLR) ile anormal hücre çoğalması ve kanser oluşumu arasında bir ilişki gösterilmiştir. Bu çalışmamızda TLR 2, TLR 4, TLR 5, TLR 6'nın sağlıklı ve kanserli endometrium dokusundaki ekspresyonunu ve karsinogenezis ile olan ilişkisini immunohistokimyasal olarak incelemeyi amaçladık. Metod: Bu çalışmaya endometrium kanseri (66 hasta, grup 1) ve endometrial hiperplazi (14 hasta, grup 2) nedeniyle histerektomi uygulanan ve endometrium kanseri dışı nedenler ile histerektomi yapılan 20 hasta (Grup 3) dahil edildi. Birinci gruptaki olgular; histolojik özellikler, kanser evresi, grade ve tümör çapı açısından sınıflandırıldı. Tüm endometrium doku örneklerinde TLR 2, TLR 4, TLR 5 ve TLR 6 ekspresyonu değerlendirildi ve bu belirteçlerin klinik ve patolojik prognostik faktörler ile arasındaki ilişki araştırıldı. İmmünohistokimyasal boyanma için nükleer ve/veya sitoplazmik boyanma pozitif reaksiyon olarak kabul edildi. Gruplar arası değerlendirmede ki-kare testi kullanıldı. p < 0.05 anlamlı kabul edildi. Bulgular: Grup 1'deki hastaların yaş ortalaması 59.8 yıl (33-83 yaş) , Grup 2 hastaların yaş ortalaması 48.3 yıl (40-59 yaş) ve 3'deki hastaların yaş ortalaması 53.4 yıl (38-84 yaş) idi. Tüm TLR türleri, endometrium kanserinden alınan doku örneklerinde yüksek oranda açığa çıkmaktadır. Ancak TLR2, grup 1'de % 87.9 açığa çıkarken, grup 2'de %100, grup 3'te % 35 oranında açığa çıkmaktadır. Bu üç grubun TLR 2 ekspresyonu açısından istatiksel karşılaştırmalarında anlamlı bir fark saptandı (p=0.000). Endometrium kanseri ve endometrial hiperplazili olgularda TLR6 ekspresyonu, kontrol grubuna göre istatiksel olarak yüksek saptandı (sırasıyla, p=0.000, p=0.000). Ayrıca ileri evre endometrium kanserinde TLR6 ekspresyonunun anlamlı olarak fazla olduğu görüldü (p=0.033). Hem TLR2 ve hem de TLR6 ile histolojik grade ve tümör boyutu arasında anlamlı bir ilişki saptanmadı. Sonuç: TLR 2 ve TLR 6 reseptörleri, endometrium kanserli ve hiperplazili olgularda kontrol grubuna göre anlamlı düzeyde yüksek oranda açığa çıkmaktadır. Ayrıca TLR 6 ekspresyonun mevcudiyeti, kanserin ileri evresini işaret edebilir.
Aim: Clinical evidence supports the association of Toll-like receptor (TLR) with abnormal cell proliferation and cancer. In this study, we aimed to investigate the expression of TLR 2, TLR4, TLR5, and TLR 6 in healthy endometrium and endometrium cancer, and to study the relation of these receptors'' expression with carcinogenesis immunohistochemically. Method: Patients who had been performed hysterectomy due to endometrium cancer (Group 1, 66 patients), endometrail hyperplasia (Group 2, 14 patients), and some reasons except endometrium cancer (Group 3, 20 patients) were included. Cases in the first group classified by histological type of the cancer, stage, grade and size of tumor. In all cases in the study, expression of TLR 2, TLR 4, TLR 5, and TLR 6 were assessed and the relations of these receptors with clinicopathologic signs were analyzed. For immunohistochemical staining, nuclear and cytoplasmic staining were considered as positive staining. Chi-squared test was used to assess the correlation of groups. P < 0.05 was considered as significant. Results: The mean age of Group 1,2 and 3 were 59.8 (33-83 years), 48.3 (40-59 years), and 53.4 (38-84 years), respectively. All types of TLR were highly expressed in both types of endometrium cancer. TLR expression was seen with a ratio of 87.9% in group 1, 100% in group 2, and 35% in group 3. There was statistically significant association in TLR2 receptor between three groups (p=0.000). TLR 6 expression in both group 1 and 2 were significantly higher than control group (p=0.000, p=0.000, respectively). In addition, TLR 6 was expressed higher in cases with late stage of cancer (p=0.033). No association was found with TLR2 and 6 regarding tumor grade and the size of the tumor. Conclusion: TLR 2 and TLR 6 were significantly more expressed in cases with endometrium cancer and endometrial hyperplasia. Presence of TLR 6 may indicate late stage of endometrial cancer.
Aim: Clinical evidence supports the association of Toll-like receptor (TLR) with abnormal cell proliferation and cancer. In this study, we aimed to investigate the expression of TLR 2, TLR4, TLR5, and TLR 6 in healthy endometrium and endometrium cancer, and to study the relation of these receptors'' expression with carcinogenesis immunohistochemically. Method: Patients who had been performed hysterectomy due to endometrium cancer (Group 1, 66 patients), endometrail hyperplasia (Group 2, 14 patients), and some reasons except endometrium cancer (Group 3, 20 patients) were included. Cases in the first group classified by histological type of the cancer, stage, grade and size of tumor. In all cases in the study, expression of TLR 2, TLR 4, TLR 5, and TLR 6 were assessed and the relations of these receptors with clinicopathologic signs were analyzed. For immunohistochemical staining, nuclear and cytoplasmic staining were considered as positive staining. Chi-squared test was used to assess the correlation of groups. P < 0.05 was considered as significant. Results: The mean age of Group 1,2 and 3 were 59.8 (33-83 years), 48.3 (40-59 years), and 53.4 (38-84 years), respectively. All types of TLR were highly expressed in both types of endometrium cancer. TLR expression was seen with a ratio of 87.9% in group 1, 100% in group 2, and 35% in group 3. There was statistically significant association in TLR2 receptor between three groups (p=0.000). TLR 6 expression in both group 1 and 2 were significantly higher than control group (p=0.000, p=0.000, respectively). In addition, TLR 6 was expressed higher in cases with late stage of cancer (p=0.033). No association was found with TLR2 and 6 regarding tumor grade and the size of the tumor. Conclusion: TLR 2 and TLR 6 were significantly more expressed in cases with endometrium cancer and endometrial hyperplasia. Presence of TLR 6 may indicate late stage of endometrial cancer.
Açıklama
Anahtar Kelimeler
Endometrial hiperplazi, Endometrial hyperplasia, Endometrial neoplazmlar, Endometrial neoplasms, Endometriyum, Endometrium, Neoplazmlar, Neoplasms, Toll like receptors, Toll like reseptörler
Kaynak
WoS Q Değeri
Scopus Q Değeri
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Künye
Bakbak, B. B. G. (2013). Endometrial hiperplaziler ve endometrium kanserinde toll-lıke reseptör ekspresyonunun klinikopatolojik değişkenler ile değerlendirilmesi. Selçuk Üniversitesi, Yayımanmış uzmanlık tezi, Konya.
