Sığırların viral solunum yolu enfeksiyonlarının serolojik olarak araştırılması
Yükleniyor...
Dosyalar
Tarih
2008-06-10
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi Fen Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu araştırmada, Konya ve çevresindeki geçmişinde solunum sistemi hastalık semptomu gösteren süt sığırlarında Infectious Bovine Rhinotracheitis (IBR), Bovine Viral Diarrhea Virus (BVDV), Bovine Respiratory Syncytial Virus (BRSV), Parainfluenza Virus tip 3 (PIV-3) ve Bovine Adenovirus tip 3 (BAV-3) enfeksiyonlarının seroprevalansı araştırıldı. Bu amaçla, özel çiftliklerde bulunan 1 yaş ve üzeri 180 hayvandan kan örneği alındı. Bu örnekler IBR, BVDV, BRSV, PIV-3 ve BAV-3' e karşı antikorların varlığı yönünden ticari olarak temin edilen Enzyme Linked Immunosorbent Assay (ELISA) ile test edildi. ELISA ile yapılan serolojik kontrol neticesinde, 46 hayvanda (% 25.55) IBR' ye karşı, 173 hayvanda (% 96.11) BVDV' ye karşı, 172 hayvanda (% 95.55) BRSV' ye karşı, 164 hayvanda (% 91.11) PIV-3' e karşı ve 150 hayvanda (% 83.33) BAV-3' e karşı spesifik antikor varlığı tespit edildi. Antikorlar yönünden test edilen sığırlarda, serumların % 1.66' sı sadece bir virus yönünden pozitifti. Serumların % 3.33' ü 2 virusa, % 17.22' si 3 virusa, % 54.44' ü 4 virusa ve % 22.77' si 5 virusa karşı antikorlara sahipti. Bununla birlikte 180 serumun % 0.55' inde antikor tespit edilmedi. Anahtar Kelimeler: süt sığırı, solunum sistemi virusları, seroloji, ELISA.
In this research, dairy cattle from Konya and its surrounding, in the past which have respiratory system symptoms were investigated for seroprevalence of the infections for Infectious Bovine Rhinotracheitis (IBR), Bovine Viral Diarrhea Virus (BVDV), Bovine Respiratory Syncytial Virus (BRSV), Parainfluenza Virus type 3 (PIV-3) and Bovine Adenovirus type 3 (BAV-3). For that purpose, one year old and over, the blood samples were taken by the 180 animals which bred in the private farms were investigated. These samples were tested for the presence of antibodies against IBR, BVDV, BRSV, PIV-3 and BAV-3 by means of a commercially available Enzyme Linked Immunosorbent Assay (ELISA). At the end of the serological control by ELISA, specific antibody presence was detected against IBR in 46 (25.55%), BVDV in 173 (96.11%), BRSV in 172 (95.55%), PIV-3 in 164 (91.11%) and BAV-3 in 150 (83.33%) animals. In the cattle tested for antibodies, 1.66% were positive for only one virus, 3.33% of sera had antibodies to two viruses, 17.22% of sera had antibodies to three viruses, 54.44% of sera had antibodies to four viruses and 22.77% of sera had antibodies to five viruses. However antibodies were not detected in 0.55% of the 180 sera.
In this research, dairy cattle from Konya and its surrounding, in the past which have respiratory system symptoms were investigated for seroprevalence of the infections for Infectious Bovine Rhinotracheitis (IBR), Bovine Viral Diarrhea Virus (BVDV), Bovine Respiratory Syncytial Virus (BRSV), Parainfluenza Virus type 3 (PIV-3) and Bovine Adenovirus type 3 (BAV-3). For that purpose, one year old and over, the blood samples were taken by the 180 animals which bred in the private farms were investigated. These samples were tested for the presence of antibodies against IBR, BVDV, BRSV, PIV-3 and BAV-3 by means of a commercially available Enzyme Linked Immunosorbent Assay (ELISA). At the end of the serological control by ELISA, specific antibody presence was detected against IBR in 46 (25.55%), BVDV in 173 (96.11%), BRSV in 172 (95.55%), PIV-3 in 164 (91.11%) and BAV-3 in 150 (83.33%) animals. In the cattle tested for antibodies, 1.66% were positive for only one virus, 3.33% of sera had antibodies to two viruses, 17.22% of sera had antibodies to three viruses, 54.44% of sera had antibodies to four viruses and 22.77% of sera had antibodies to five viruses. However antibodies were not detected in 0.55% of the 180 sera.
Açıklama
Anahtar Kelimeler
Süt sığırı, Dairy cattles, Solunum sistemi virusları, Viruses of the respiratory system, Seroloji, Serology, ELISA
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Gürses, E. (2008). Sığırların viral solunum yolu enfeksiyonlarının serolojik olarak araştırılması. Selçuk Üniversitesi, Yayımlanmış yüksek lisans tezi, Konya.
