Theileria equi seropozitif ve seronegatif atların serum lipid ve protein profilleri
Yükleniyor...
Tarih
2011
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışma Theileria equi seropozitif ve seronegatif atlar üzerinde, serum protein ve lipid profillerini ortaya koymak amacıyla yürütülmüştür. Gereç ve Yöntem: Theileria equi seropozitif ve seronegatif atlar IFAT ile test edilerek belirlendi. Bu atlara ait kan se- rumlarında SDS-PAGE ve HPTLC analizleri yapılarak sıra- sıyla serum protein ve lipit profilleri ortaya çıkarıldı ve bu analiz sonuçlarına göre seropozitif ve seronegatif atlar ara- sındaki farklılıklar ortaya konulmaya çalışıldı. Bulgular: Seropozitif hayvanların 23, 52, 103, 176, 200 ve 240 kDa ağırlığındaki proteinlerinde önemli oranda artış olduğu tespit edildi. Ayrıca hem sağlıklı hem de T. equi se- ropozitif hayvanların albumin nktarları benzer düzeyler- de olmasına karşın T. equi seropozitif hayvanların globulin miktarlarının arttığı görüldü. Serum lipid profilinde ise se- ropozitif hayvanların genel bir lipoliz durumunda oldukları, toplam lipid miktarı içinde özellikle hidrokarbon oranının azaldığı ve diacilgliserol düzeyinin arttığı belirlendi. Yine seropozitif hayvanlarda fosfolipid miktarında hafif artış ve kolesterol oranında ise hafif bir düşme tespit edildi. Öneri: Protein ve lipid profillerindeki bu değişiklikler, hay- vanların akut enfeksiyonu atlatmalarına rağmen hala tam olarak iyileşemediklerini göstermektedir.
Aim: This study aims at determining serum protein and li- pid profiles in Theileria equi seropositive and seronegative horses. Materials and Methods: Theileria equi seropositivity and seronegativity at horses were tested by IFAT Having ana- lyzed the blood sera of horses by SDS-PAGE and HPTLC, se- rum protein and lipid profiles were detected respectively. Differences between seropositive and seronegative horses were tried to be determined based on the results obtained by said analysis. Results: It was detected that there was a significant in- crease in 23, 52, 103, 176, 200 and 240 kDa protein levels of seropositive horses. Additionally, globulin levels of T. equi seropositive animals were observed to have increased, al- though albumin levels of both healthy and T. equi seroposi- tive horses were similar. At serum lipid profile, seropositive horses were observed to be at overall lipolysis; among the total amount of lipid, particularly reduced hydrocarbon rates as well as increased diacylglycerol levels were detect- ed. A slight increase in phospholipids and a slight decrease in cholesterol levels were found in seropositive horses. Conclusion: Mentioned changes at both protein and lipid profiles suggest that these horses have not fully recovered despite the fact that they survived acute infection.
Aim: This study aims at determining serum protein and li- pid profiles in Theileria equi seropositive and seronegative horses. Materials and Methods: Theileria equi seropositivity and seronegativity at horses were tested by IFAT Having ana- lyzed the blood sera of horses by SDS-PAGE and HPTLC, se- rum protein and lipid profiles were detected respectively. Differences between seropositive and seronegative horses were tried to be determined based on the results obtained by said analysis. Results: It was detected that there was a significant in- crease in 23, 52, 103, 176, 200 and 240 kDa protein levels of seropositive horses. Additionally, globulin levels of T. equi seropositive animals were observed to have increased, al- though albumin levels of both healthy and T. equi seroposi- tive horses were similar. At serum lipid profile, seropositive horses were observed to be at overall lipolysis; among the total amount of lipid, particularly reduced hydrocarbon rates as well as increased diacylglycerol levels were detect- ed. A slight increase in phospholipids and a slight decrease in cholesterol levels were found in seropositive horses. Conclusion: Mentioned changes at both protein and lipid profiles suggest that these horses have not fully recovered despite the fact that they survived acute infection.
Açıklama
Anahtar Kelimeler
Theileria equi, IFAT, SDS-PAGE, HPTLC
Kaynak
Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
27
Sayı
2
Künye
Balkaya, İ., Kaynar, Ö., Ütük, A. E., Pişkin, F. Ç., (2011). Theileria Equi Seropozitif Ve Seronegatif Atların Serum Lipid ve Protein Profilleri. Eurasian Journal of Veterinary Sciences, 27, (2), 117-122.
