Comparison of blv antibodies in late pregnant and non-pregnant holstein cows using serum and milk elisa
Küçük Resim Yok
Tarih
2007
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada, Bovine lökemi virus (BLV) enfeksiyonunun Konya bölgesindeki prevalansı araştırıldı. Bu nedenle, ileri gebelik döneminde ve gebe olmayan Holstein ırkı sığırların kan ve süt serumu örneklerinde BLV antikorlarının Enzim linked immunosorbent assay (ELISA) ile karşılaştırılması için 1948 adet kan ve süt örneği incelendi. Ayrıca yaşa göre her iki grupta değerlendirme yapıldı. İleri gebelik döneminde bulunan süt sığırlarında BLV enfeksiyonunun prevalansı serum-ELISA ile % 4.41 ve süt-ELISA ile % 5.74 olarak belirlendi. Diğer yandan gebe olmayan süt sığırlarında BLV enfeksiyonunun prevalansı serum-ELISA (% 4.72) ile süt-ELISA (% 4)'dan daha yüksek bulundu. Hem ileri gebelik döneminde (% 96.81) hem de gebe olmayan (% 97.02) süt sığırlarında serum-ELISA ile süt-ELISA arasında Önemli bir korrelasyon vardı. Hem ileri gebelik döneminde hem de gebe olmayan süt sığırlarında en yüksek BLV enfeksiyon oranı 6 yaşlı hayvanlarda tespit edildi. Sonuç olarak, gebe olmayan hayvanlarda BLV enfeksiyonunun teşhisinde serum-ELISA, süt-ELISA'ya göre daha duyarlı bulundu. Bununla birlikte, ileri gebelik döneminde bulunan hayvanlarda BLV enfeksiyonunun teşhisinde süt-ELISA, serum-ELISA'ya göre daha duyarlı bulundu.
Prevalence of Bovine leukemia virus (BLV) infection in Konya region was investigated in this study. For this reason, 1948 blood and milk samples were tested with serum and milk Enzyme linked immunosorbent assay (ELISA) in late pregnant and non-pregnant Holstein cows to compare the effectiveness of serum and milk ELISA on detecting BLV antibodies in cows with different physiological stages. Moreover, cows were divided into two groups according to their ages. In late pregnant dairy cows, prevalence of BLV infection was 4.41 and 5.74% with serum-ELISA and milk-ELISA, respectively. Prevalence of BLV infection, on the other hand, was found greater with serum-ELISA (4.72%) than milk-ELISA (4%) in non-pregnant dairy cows. There was a significant correlation between serum-ELISA and milk-ELISA for both late pregnant dairy cows (96.81%) and non-pregnant dairy cows (97.02%). The BLV infection rates for both late pregnant and non-pregnant dairy cows were the highest in the cows at 6 years of age. In conclusion, serum-ELISA test was more sensitive than milk-ELISA to diagnose BLV infection in non-pregnant animals. However, to diagnose BLV infection in late pregnant animals, milk-ELISA test was more sensitive than serum-ELISA.
Prevalence of Bovine leukemia virus (BLV) infection in Konya region was investigated in this study. For this reason, 1948 blood and milk samples were tested with serum and milk Enzyme linked immunosorbent assay (ELISA) in late pregnant and non-pregnant Holstein cows to compare the effectiveness of serum and milk ELISA on detecting BLV antibodies in cows with different physiological stages. Moreover, cows were divided into two groups according to their ages. In late pregnant dairy cows, prevalence of BLV infection was 4.41 and 5.74% with serum-ELISA and milk-ELISA, respectively. Prevalence of BLV infection, on the other hand, was found greater with serum-ELISA (4.72%) than milk-ELISA (4%) in non-pregnant dairy cows. There was a significant correlation between serum-ELISA and milk-ELISA for both late pregnant dairy cows (96.81%) and non-pregnant dairy cows (97.02%). The BLV infection rates for both late pregnant and non-pregnant dairy cows were the highest in the cows at 6 years of age. In conclusion, serum-ELISA test was more sensitive than milk-ELISA to diagnose BLV infection in non-pregnant animals. However, to diagnose BLV infection in late pregnant animals, milk-ELISA test was more sensitive than serum-ELISA.
Açıklama
Anahtar Kelimeler
Veterinerlik
Kaynak
Veterinarium
WoS Q Değeri
Scopus Q Değeri
Cilt
18
Sayı
1
