Determinations of mRNA's For TGF beta(2,3) in Mouse Testes and Ovaries After a Five days Injection With Glycine or a Serine and Threonine Mixture
Küçük Resim Yok
Tarih
2008
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
MEDWELL ONLINE
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
This study is presented to test the effect of injecting a mixture of Serine/Threonine or Glycine amino acids to determine and measure the changes in mRNAs expression for TGF beta(2.3) within the mouse testes and the ovaries. Male (n = 30) and female (n = 30) mice (CD-1 strain) were injected, once a day for 5 days, with 0.2 mL Saline (control), 0.2 mL Saline with a mixture of Serine/Threonine (test) containing 0.26 mu g L Threonine, 0.13 mu g L Serine or 38 ng Glycine (Test). Total RNA was extracted and transferred on to a nylon membrane then probed with P-32-lebelled cDNA probes. The expression of mRNA for TGF beta(2) increased in male mice while there were no differences in female mice. This study shows that TGF beta(2.3) are expressed both in the ovary and testis and their expressions were increased by the injection of Serine/Threonine or glycine.
Açıklama
Anahtar Kelimeler
Mice, RNA, TGF beta, serine, threonine, glycine
Kaynak
JOURNAL OF ANIMAL AND VETERINARY ADVANCES
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
7
Sayı
1
