EFFICIENT REGENERATION AND AGROBACTERIUM TUMEFACIENS MEDIATED GENETIC TRANSFORMATION OF POTATO (SOLANUM TUBEROSUM L.)
Küçük Resim Yok
Tarih
2018
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
PARLAR SCIENTIFIC PUBLICATIONS (P S P)
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Potato (Solanum tuberosum L.) is highly demandable world food crop and has become a staple food in many parts of the world including Turkey, India and China. Potato under in vitro culture condition is recalcitrant and genotype dependent. Therefore, we tested several factors to enhance regeneration and genetic transformation efficiency of Turkish potato genotypes. The influence of physical factors and conditions in Agrobacterium mediated transformation of the potato leaves was tested. Using the best combination (10 mg L-1 ZR, 10 mg L-1 NAA) with Agrobacterium-mediated transformation, we regenerated independent putative transformants 61.6% of the total explants cultured. GUS and nptII positive plants were confirmed by molecular analysis (PCR) that showed transformation rate of 24.16%. The protocol described here is simple, efficient, and produces transgenic plant in 7-8 weeks after inoculation with Agrobacterium bacterium.
Açıklama
Anahtar Kelimeler
Solanum tuberosum, Agrobacterium tumefaciens, Transformation, potato, Co-cultivation
Kaynak
FRESENIUS ENVIRONMENTAL BULLETIN
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
27
Sayı
5
