Synthesis and application of calix[4]arene derivatives bearing pyridyl and nitro groups, in the lipase-catalyzed enantioselective reactions
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Dosyalar
Tarih
2015-06-17
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi Fen Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada nitro ve piridil gruplarının etkisinin lipaz katalizli enantiyoseçimli tepkimedeki etkilerini gözlemlemek için, nitro, piridil ve her iki grubu bulunduran kaliks[4]aren türevleri (5, 6, 9, 10) sentezlendi. Sentezlenen bileşiklerin yapıları spektroskopik teknikler (FTIR, 1H NMR ve 13C- NMR) ile aydınlatıldı. Sonra foksiyonlu kaliks[4]aren bileşikleri Fe3O4 varlığında lipaz immobilizasyonu yapılarak rasemik flurbiprofen metil esterinin enantiyoseçimli hidroliz tepkimelerinde kullanıldı. Reaksiyonlar normal faz kiral kolon kullanılarak ürünlerin enantiyomerik kompozisyon analizi ve enantiyomerik fazlalıkları sıvı kromatografisi tekniği (HPLC) ile yapıldı.
In order to determine the effects of nitro and pyridyl moieties on the lipase catalyzed enantioselective reactions, various calix[4]arene derivatives-functionalized with nitro, pyridine and both of functional groups (5,6,9,10) have been synthesized. Structures of all synthesized compounds have been characterized using spectroscopic techniques (FTIR, 1H NMR and 13C- NMR). Different functionalized calix[4]arene derivatives and Fe3O4 magnetic nanoparticles were used as additives for the encapsulation of lipase. Then, these encapsulated lipases were performed as effective catalysts for the enantioselective hydrolysis reaction of (R/S)-flurbiprofen methyl ester. Moreover, the effects of reaction parameters such as temperature and pH on the catalytic affinities of the encapsulated lipases were also discussed.
In order to determine the effects of nitro and pyridyl moieties on the lipase catalyzed enantioselective reactions, various calix[4]arene derivatives-functionalized with nitro, pyridine and both of functional groups (5,6,9,10) have been synthesized. Structures of all synthesized compounds have been characterized using spectroscopic techniques (FTIR, 1H NMR and 13C- NMR). Different functionalized calix[4]arene derivatives and Fe3O4 magnetic nanoparticles were used as additives for the encapsulation of lipase. Then, these encapsulated lipases were performed as effective catalysts for the enantioselective hydrolysis reaction of (R/S)-flurbiprofen methyl ester. Moreover, the effects of reaction parameters such as temperature and pH on the catalytic affinities of the encapsulated lipases were also discussed.
Açıklama
Anahtar Kelimeler
İmmobilize enzimler, Immobilized enzyme
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Doğan, V. (2015). Synthesis and application of calix[4]arene derivatives bearing pyridyl and nitro groups, in the lipase-catalyzed enantioselective reactions. Selçuk Üniversitesi, Yayımlanmış yüksek lisans tezi, Konya.
