Binding Affinity of Serum Proteins to Epoxy Modified Magnetite Nanoparticles

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dc.contributor.authorMaltaş, Esra
dc.contributor.authorÖzmen, Mustafa
dc.contributor.authorYıldız, Salih
dc.contributor.authorErsöz, Mustafa
dc.date.accessioned2020-03-26T18:32:37Z
dc.date.available2020-03-26T18:32:37Z
dc.date.issued2012
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractSuperparamagnetic iron oxide nanoparticles (SPIONs) were synthesized by co-precipitating Fe2+ and Fe3+ in an ammonia solution. SPIONs modified with [3-(2,3-epoxypropoxy)propyl] trimethoxy silane, which resulted in the presence of a high population of epoxy groups on the particles surface. The surface functional SPIONs can be bound with active bio-substance, and have a wide application prospect in the fields of biology and medicine. The binding percentage of proteins was studied with epoxy modified SPIONs. Several serum proteins including albumin, ?-globulin, hemoglobin, cytochrome c and myoglobin were studied for immobilization. Epoxy terminated SPIONs were shown the highest binding ability for immobilization of cytochrome c and hemoglobin with 1 and 0.86 mg total protein at a particle concentration of 20 mg/mL among proteins, respectively. This binding ability was also shown by using sodium dodecylsulfate polyacrylamide gel electrophoresis technique (SDS-PAGE). Protein attachments were also examined by Scanning Electron Microscopy (SEM). © 2012 American Scientific Publishers. All rights reserved.en_US
dc.identifier.citationMaltas, Esra., Özmen, Mustafa., Yıldız, Salih., Ersöz, Mustafa., (2012). Binding Affinity of Serum Proteins to Epoxy Modified Magnetite Nanoparticles. Advanced Science Letters, 17(1), 143-148.
dc.identifier.doi10.1166/asl.2012.4242en_US
dc.identifier.endpage148en_US
dc.identifier.issn1936-6612en_US
dc.identifier.issue1en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage143en_US
dc.identifier.urihttps://dx.doi.org/10.1166/asl.2012.4242
dc.identifier.urihttps://hdl.handle.net/20.500.12395/28736
dc.identifier.volume17en_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorMaltas, Esra.
dc.institutionauthorÖzmen, Mustafa.
dc.institutionauthorYıldız, Salih.
dc.institutionauthorErsöz, Mustafa.
dc.language.isoenen_US
dc.relation.ispartofAdvanced Science Lettersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectCytochrome Cen_US
dc.subjectEpoxyen_US
dc.subjectHemoglobineen_US
dc.subjectMagnetic Nanoparticlesen_US
dc.subjectSds-Pageen_US
dc.titleBinding Affinity of Serum Proteins to Epoxy Modified Magnetite Nanoparticlesen_US
dc.typeArticleen_US

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