Intraoral premalign ve malign lezyonlu hastaların doku örneklerindeki candida türlerinin DNA analiziyle belirlenmesi ve sağlıklı doku ile karşılaştırılması
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Tarih
2019
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu retrospektif çalışmada premalign ve malign lezyonlu ile sağlıklı bireylere ait doku örneklerindeki Candida albicans, Candida kruseii, Candida parapsilosis, Candida tropicalis ve Candida glabrata türlerinin belirlenmesi amaçlanmıştır. Gereç ve Yöntem: Çalışmaya oral premalign veya malign lezyon şüphesi nedeniyle biyopsi uygulanmış doku örnekleri dahil edildi. Örnekler histolojik özelliklerine göre sağlıklı (n=20), displazi (n=20), karsinoma in-situ (n=20) ve skuamoz hücreli karsinom (SHK) (n=20) olmak üzere 4 gruba ayrıldı. DNA izolasyonunun ardından Candida türlerine ait DNA örneklerinin miktar ve saflık kontrolleri gerçekleştirildi. Kantitatif ölçümler için Real-Time PCR (LightCycler 96, Almanya) kullanıldı ve standart eğriler hesaplandı. Veriler Mann-Whitney ve Wilcoxon Signed Ranks testleri ile değerlendirildi (P<0.05). Bulgular: Sağlıklı ve displazik örneklerin yer aldığı gruplarda C. parapsilosis (104DNA/50 mg) en sık görülen Candida türüydü. Karsinoma in-situ grubunda C. tropicalis ve C. kruseii (103DNA/50 mg), SHK grubunda ise C. kruseii (104DNA/50 mg) seviyelerinin daha yüksek olduğu belirlendi. Örneklerin hiçbirinde C. albicans varlığı gösterilemedi. Gruplar arasında farklı Candida türlerinin sayısına göre anlamlı bir fark gözlenmedi (P>0.05). Farklı Candida türlerinin gruplar içerisindeki seviyeleri incelendiğinde; sağlıklı örneklerde C. glabrata (103DNA/50 mg), displazi grubunda C. parapsilosis (104DNA/50 mg), karsinoma insitu grubunda C. tropicalis-C. kruseii (103DNA/50 mg), SHK grubunda ise C. kruseii’nin (103DNA/50 mg) en fazla sayıda gözlenen Candida türü olduğu belirlendi. Candida türlerinin gruplar içerisindeki dağılımı anlamlı değildi (P>0.05). Sonuç: Sağlıklı, displazik, karsinoma in situ ve skuamöz hücreli karsinoma doku örneklerinde Real-Time PCR yöntemiyle yapılan kantitatif değerlendirmede Candida türlerinin tüm gruplarda benzer oranda mevcut oldukları belirlendi.
Background: Aim of this retrospective study is to determine level of Candida albicans, Candida kruseii, Candida parapsilosis, Candida tropicalis, Candida glabratain samples of patients with premalignant, malignant or benign lesions. Methods: Samples biopsied due to suspicion of premalignant or malignant lesions were included. They were divided into 4 groups: healthy (n=20), dysplasia (n=20), carcinoma in-situ (n=20) and squamous cell carcinoma (SCC) (n=20). Following DNA extraction, DNA samples were examined for quantity and quality. Quantitative measurements were performed using RealTime PCR (LightCycler 96, Germany), standard curves were calculated. Data was analyzed using Mann-Whitney and Wilcoxon Signed Ranks tests (P<0.05). Results: C. parapsilosis (104DNA/50 mg) was the most common Candida species among healthy and dysplasia groups. Level of C. tropicalis and C. kruseii (103DNA/50 mg) was higher in carcinoma in-situ samples, C. kruseii (104DNA/50 mg) was higher among SCC. Presence of C. albicans was not detected in any of the samples. No significant difference regarding level of different Candida species (P>0,05) were observed. When level of Candida was examined within each group, amount of C. glabrata (103DNA/50 mg), C. parapsilosis (104DNA/50 mg), C. tropicalis-C. kruseii (103DNA/50 mg), C. kruseii (103DNA/50 mg) was higher among healthy, dysplasia, carcinoma in-situ and SCC groups respectively. No significant differences were observed regarding level of Candida species within each group (P>0.05). Conclusion: The quantitative evaluation of Candida in healthy, dysplasia, carcinoma in-situ and squamous cell carcinoma samples using Real-Time PCR revealed similar levels of Candida among all groups.
Background: Aim of this retrospective study is to determine level of Candida albicans, Candida kruseii, Candida parapsilosis, Candida tropicalis, Candida glabratain samples of patients with premalignant, malignant or benign lesions. Methods: Samples biopsied due to suspicion of premalignant or malignant lesions were included. They were divided into 4 groups: healthy (n=20), dysplasia (n=20), carcinoma in-situ (n=20) and squamous cell carcinoma (SCC) (n=20). Following DNA extraction, DNA samples were examined for quantity and quality. Quantitative measurements were performed using RealTime PCR (LightCycler 96, Germany), standard curves were calculated. Data was analyzed using Mann-Whitney and Wilcoxon Signed Ranks tests (P<0.05). Results: C. parapsilosis (104DNA/50 mg) was the most common Candida species among healthy and dysplasia groups. Level of C. tropicalis and C. kruseii (103DNA/50 mg) was higher in carcinoma in-situ samples, C. kruseii (104DNA/50 mg) was higher among SCC. Presence of C. albicans was not detected in any of the samples. No significant difference regarding level of different Candida species (P>0,05) were observed. When level of Candida was examined within each group, amount of C. glabrata (103DNA/50 mg), C. parapsilosis (104DNA/50 mg), C. tropicalis-C. kruseii (103DNA/50 mg), C. kruseii (103DNA/50 mg) was higher among healthy, dysplasia, carcinoma in-situ and SCC groups respectively. No significant differences were observed regarding level of Candida species within each group (P>0.05). Conclusion: The quantitative evaluation of Candida in healthy, dysplasia, carcinoma in-situ and squamous cell carcinoma samples using Real-Time PCR revealed similar levels of Candida among all groups.
Açıklama
Anahtar Kelimeler
Oral Candida, C. albicans, ağız kanseri, Real-Time PCR, moleküler analiz, Oral Candida, oral cancer, molecular analyses
Kaynak
Selçuk Üniversitesi Diş Hekimliği Fakültesi Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
6
Sayı
4
Künye
Karaca, B., Güneri, P., Vural, C., Diallo, M. M., Özdemir, G., Gürhan, C., Aykutlu, U., (2019). Intraoral premalign ve malign lezyonlu hastaların doku örneklerindeki candida türlerinin DNA analiziyle belirlenmesi ve sağlıklı doku ile karşılaştırılması. Selçuk Üniversitesi Diş Hekimliği Fakültesi Dergisi, 6 (4), 269-273.
