Timokuinonun sığır periferal kan monükleer hücre proliferasyonuna etkisi
Küçük Resim Yok
Tarih
2018
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Timokuinon (Tq), Nigella sativa'nın en önemli bileşeni olup memelilerin çeşitli hücreleri üzerinde olumlu biyolojik etkilere sahiptir. Bu çalışmada, sığırların periferal kan dolaşımı mononükleer hücreleri üzerine Tq'nin in vitro şartlarda etkisi araştırıldı.Gereç ve Yöntem: Farklı Tq yoğunluklarının sığır periferal kan dolaşımı mononükleer hücrelerinin yaşamına ve proliferasyonuna etkileri hücre kültürü (BrdU) ve ELISA metotları ile ve iki mitojenin (Concanavalin A; Con A ve Pokeweed Mitogen; PWM) varlığında ve yokluğunda araştırıldı. Bununla birlikte fenil boronik asit (FBA)'in muhtemel etkisi benzer şekilde ölçüldü.Bulgular: Tq, sığır kan dolaşımında bulunan mononükleer hücreler, kültür sıvısına 50 µg/mL miktarında eklendiğinde blastojenik etki gösterdi. 5 günlük 10?/mL yoğunluğunda hücre bulunan kültürlerde 50 µg/mL Tq, 0.4850.06 düzeyinde blastogenetik cevaba sebep oldu (P0,01). Con A ve PWM için mitojenik cevaplar ise sırası ile 0,3990,084 ve 0,3970,049 olarak belirlendi. Tq'nun her iki mitojen (Con A veya PWM) ile kombinasyonu blastogenetik yanıtta artışa yol açmadı. Ancak, Tq'nun immün hücreler üzerindeki bu mitojenik etkisinin mekanizması aydınlatılmaya muhtaçtır. FBA de, kültüre 2.5 µg/mL son konsantrasyonunda eklendiğinde anlamlı miktarda (0,4250,058) mitojenik etki gösterdi (P0,01)Öneri: Tq'nun, gelecekte adjuvan bileşimine girebilecek aday bir madde olduğu fikrimizi, ayrıca doğrudan ve in vivo olarak immün uyarıcı olarak da kullanılabileceği hipotezimizi desteklemektedir. Diğer taraftan, FBA'nın da, sığırda benzer potansiyele sahip olabileceği değerlendirilmektedir.
Aim: Thymoquinone (Tq) is the most important component of Nigella sativa and has positive biological effects on various cells of the mammals. At present study, a possible effect of Tq on the large ruminant immune system was investigated by in vitro assays. Materials and Methods: Thus, the effects of different Tq concentrations on both survival and proliferation of bovine peripheral blood mononuclear cells were investigated by cell culture and ELISA methods in the presence or absence of two mitogens (Con A and PWM). In addition, phenyl boronic acid (PBA) was evaluated similarly.Results: Tq showed a blastogenic effect on peripheral mononuclear cells of cows when Tq was added to culture medium at a final concentration of 50 ?g/mL. Following 5 days of proliferation of cells with 10? cell/mL density in presence of 50 ?g/mL Tq caused 0.485 0.06 OD degree of blastogenesis (P 0.01). The mitogenic responses for Con A and PWM were 0,399 0,084 and 0,397 0,049, respectively. The combination of Tq with either of both mitogens (Con A or PWM) did not lead to a further increase in the proliferation. Mechanism of Tq's mitogenic effect on bovine immune cells is completely in dark at present. Additionally, PBA is present in cell culture medium at a final concentration of 2.5 µg/mL a significant degree of proliferation was measured (0,4250,058) (P0,01).Conclusions: These results support our hypothesis that Tq would be a candidate for being a component of a novel adjuvant or even a promising immunostimulant for therapeutic purposes in future. Moreover, PBA is concluded as another potential compound for mitogenesis of bovine immune cells
Aim: Thymoquinone (Tq) is the most important component of Nigella sativa and has positive biological effects on various cells of the mammals. At present study, a possible effect of Tq on the large ruminant immune system was investigated by in vitro assays. Materials and Methods: Thus, the effects of different Tq concentrations on both survival and proliferation of bovine peripheral blood mononuclear cells were investigated by cell culture and ELISA methods in the presence or absence of two mitogens (Con A and PWM). In addition, phenyl boronic acid (PBA) was evaluated similarly.Results: Tq showed a blastogenic effect on peripheral mononuclear cells of cows when Tq was added to culture medium at a final concentration of 50 ?g/mL. Following 5 days of proliferation of cells with 10? cell/mL density in presence of 50 ?g/mL Tq caused 0.485 0.06 OD degree of blastogenesis (P 0.01). The mitogenic responses for Con A and PWM were 0,399 0,084 and 0,397 0,049, respectively. The combination of Tq with either of both mitogens (Con A or PWM) did not lead to a further increase in the proliferation. Mechanism of Tq's mitogenic effect on bovine immune cells is completely in dark at present. Additionally, PBA is present in cell culture medium at a final concentration of 2.5 µg/mL a significant degree of proliferation was measured (0,4250,058) (P0,01).Conclusions: These results support our hypothesis that Tq would be a candidate for being a component of a novel adjuvant or even a promising immunostimulant for therapeutic purposes in future. Moreover, PBA is concluded as another potential compound for mitogenesis of bovine immune cells
Açıklama
Anahtar Kelimeler
Veterinerlik
Kaynak
Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
34
Sayı
1
