Oral olarak aflatoksin B1 verilen ratlarda kurkuminin bazı karaciğer enzimleri, sitokinler ile böbrek fonksiyonları üzerine etkisi
Yükleniyor...
Dosyalar
Tarih
2021
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi Sağlık Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu araştırmada, ratlarda oral Aflatoksin B1 (AFB1) uygulması ile deneysel olarak oluşturulan kronik aflatoksikozise karşı kurkuminin bazı karaciğer enzimleri, antioksidanlar, sitokinler ve böbrek fonksiyonları üzerine etkisinin belirlenmesi amaçlanmıştır. Araştırma süresi 60 gün tutuldu ve bu süreçte 2 haftalık, sağlıklı 38 adet Wistar albino rat kullanıldı. Çalışmaya başlamadan önce hayvanların genel sağlık durumları gözden geçirilerek, canlı ağırlıkları belirlendi ve ortalama canlı ağırlıklarına göre; Kontrol (K), Dimetilsülfoksit (D), Kurkumin (Kur), Aflatoksin B1 (AF) ve AFB1 + Kurkumin (AF +Kur) olmak üzere 5 gruba ayrıldı. D grubundaki ratlara oral olarak %10'lik DMSO uygulandı. Kur ve AF+Kur grubundaki ratlara %10'lik DMSO'da çözdürülen 300 mg/kg kurkumin oral olarak uygulandı. AF ve AF+Kur grubundaki ratlara%10'lik DMSO'da çözdürülen 250 µg/kg AFB1 uygulandı. Araştırmada sonunda gruplardaki deneklerden elde edilen kan örneklerinden MDA, SOD, GSH, ALT, AST, ALP, GGT, BUN, ürik asit, Kreatinin, IL-1β, IL-6 ve TNF-α konsantrasyonları belirlendi. MDA düzeyinin AF grubunda diğer dört gruba göre anlamlı olarak arttığı (p<0.05), eş zamanlı kurkumin uygulamasından sonra ise AF+Kur grubunda belirlenen MDA düzeyi AF grubundan istatistiksel olarak düşük olduğu (p<0.05) belirlendi. GSH düzeyinin istatistiksel olarak en düşük AF grubunda olduğu gözlendi (p<0.05). AF+Kur grubunda ise GSH düzeyinin AF grubundan anlamlı olarak yüksek olduğu belirlendi (p<0.05). SOD düzeyi ise AF grubunda tüm gruplardan anlamlı düzeyde daha düşük (p<0.05), AF+Kur grubunda ise AF grubu ile kıyaslandığında anlamlı derecede daha yüsek olarak bulundu (p< 0.05). AF grubunda IL-6 ve TNF-α düzeyleri diğer dört gruptan anlamlı düzeyde daha yüksek olduğu belirlendi (p< 0.05). AF+Kur grubunda ise IL-6 ve TNF-α düzeyi AF grubuna göre anlamlı düzeyde daha düşük olarak gözlendi (p< 0.05). IL-1β seviyesi ise AF+Kur grubunda, AF grubuna göre azalma eğilimi göstermekle birlikte istatistiksel olarak bu azalma anlamlı değildi (p>0.05). AF grubunda AST, ALT, ALP ve GGT düzeyleri diğer gruplarla karşılaştırıldığında istatistiksel olarak daha yüksekti (p<0.05). Afla+Kur grubu plazma AST, ALT, ALP ve GGT düzeyleri ise AF grubu ile karşılaştırıldığında istatistiksel olarak önemli düzeyde düşük olduğu belirlendi (p<0.05). BUN, Ürik asit ve Kreatinin düzeylerinin AF grubunda K, Kur ve D gruplarına göre istatistiksel olarak yüksek olduğu gözlemlendi (p<0.05). AF+Kur grubundan elde edilen verilerin AF grubuna göre Kreatinin düzeyinde herhangi bir fark gözlemlenmezken (p>0.05) , BUN düzeyinde düşme eğilimi, ürik asit düzeyinin ise anlamlı düzeyde düşük olduğu tespit edildi (p<0.05). Sonuç olarak AFB1 ile deneysel kronik aflatoksikozis oluşturulan ratlarda karaciğer ve böbrek hasarını iyileştirmek, inflamasyonu kontrol altına almak, oksidatif stresi azaltmak ve antioksidan savunma sistemini desteklemek amacıyla iyi bir antioksidan ve anti-infalmatuar olarak bilinen kurkuminin, incelenen parametreler açısından AFB1 toksisitesinin zararlı etkilerini önlemede veya hafifletmede oldukça faydalı olabileceği kanaatine varıldı.
In this study, it was aimed to determine the effect of curcumin on some liver enzymes, cytokines and kidney functions against chronic aflatoxicosis created by oral Aflatoxin B1 (AFB1) administration in rats. The duration of the study was 60 days and 38 healthy 2-week-old Wistar albino rats were used during this period. Before starting the study, the general health conditions of the animals were reviewed, their body weight was determined, and they were divided into 5 groups according to their average body weight. Control (K), Dimethylsulfoxide (D), Curcumin (Kur), AFB1 (AF) and AFB1 + Curcumin (AF + Kur). 10% DMSO was administered orally to the rats in group D. 300 mg/kg curcumin dissolved in 10% DMSO was administered orally to the rats in the Kur and AF + Kur groups. 250 µg / kg AFB1 dissolved in 10% DMSO was administered to the rats in AF and AF + Kur groups. At the end of the study, MDA, SOD, GSH, ALT, AST, ALP, GGT, BUN, urea, creatinine, IL-1β, IL-6 and TNF-α concentrations were determined from the blood samples obtained from the subjects in the groups. It was determined that the MDA level in the AF group increased significantly compared to the other four groups (p <0.05), and after the simultaneous application of curcumin, the MDA level determined in the AF + Kur group was statistically lower than the AF group (p <0.05). It was observed that the GSH level was statistically the lowest in the AF group (p <0.05). In the AF + Kur group, the GSH level was found to be significantly higher than in the AF group (p <0.05). The SOD level was significantly lower in the AF group than all groups (p <0.05), and significantly higher in the AF + Kur group compared to the AF group (p <0.05). IL-6 and TNF-α levels were found to be significantly higher in the AF group than in the other four groups (p <0.05). In the AF + Kur group, IL-6 and TNF-α levels were significantly lower than in the AF group (p <0.05). IL-1β level tended to decrease in AF + Kur group compared to the AF group, but this decrease was not statistically significant (p> 0.05). AST, ALT, ALP and GGT levels were statistically higher in the AF group compared to other groups (p <0.05). The plasma AST, ALT, ALP and GGT levels of the Afla + Kur group were found to be significantly lower than the AF group (p <0.05). It was observed that BUN, Uric acid and Creatinine levels were statistically higher in the AF group compared to K, Kur and D groups (p <0.05). While the data obtained from AF + Kur group did not show any difference in creatinine level compared to the AF group (p> 0.05), it was found that the BUN level decreased,and the uric acid level was significantly lower (p <0.05). In conclusion, it was concluded that curcumin, which is known as a good antioxidant and anti-inflammatory system to heal liver and kidney damage, control inflammation, reduce oxidative stress and support the antioxidant defence system in rats with experimental chronic aflatoxicosis with AFB1 may be very useful in preventing or mitigating the harmful effects of AFB1 toxicity in terms of parameters examined.
In this study, it was aimed to determine the effect of curcumin on some liver enzymes, cytokines and kidney functions against chronic aflatoxicosis created by oral Aflatoxin B1 (AFB1) administration in rats. The duration of the study was 60 days and 38 healthy 2-week-old Wistar albino rats were used during this period. Before starting the study, the general health conditions of the animals were reviewed, their body weight was determined, and they were divided into 5 groups according to their average body weight. Control (K), Dimethylsulfoxide (D), Curcumin (Kur), AFB1 (AF) and AFB1 + Curcumin (AF + Kur). 10% DMSO was administered orally to the rats in group D. 300 mg/kg curcumin dissolved in 10% DMSO was administered orally to the rats in the Kur and AF + Kur groups. 250 µg / kg AFB1 dissolved in 10% DMSO was administered to the rats in AF and AF + Kur groups. At the end of the study, MDA, SOD, GSH, ALT, AST, ALP, GGT, BUN, urea, creatinine, IL-1β, IL-6 and TNF-α concentrations were determined from the blood samples obtained from the subjects in the groups. It was determined that the MDA level in the AF group increased significantly compared to the other four groups (p <0.05), and after the simultaneous application of curcumin, the MDA level determined in the AF + Kur group was statistically lower than the AF group (p <0.05). It was observed that the GSH level was statistically the lowest in the AF group (p <0.05). In the AF + Kur group, the GSH level was found to be significantly higher than in the AF group (p <0.05). The SOD level was significantly lower in the AF group than all groups (p <0.05), and significantly higher in the AF + Kur group compared to the AF group (p <0.05). IL-6 and TNF-α levels were found to be significantly higher in the AF group than in the other four groups (p <0.05). In the AF + Kur group, IL-6 and TNF-α levels were significantly lower than in the AF group (p <0.05). IL-1β level tended to decrease in AF + Kur group compared to the AF group, but this decrease was not statistically significant (p> 0.05). AST, ALT, ALP and GGT levels were statistically higher in the AF group compared to other groups (p <0.05). The plasma AST, ALT, ALP and GGT levels of the Afla + Kur group were found to be significantly lower than the AF group (p <0.05). It was observed that BUN, Uric acid and Creatinine levels were statistically higher in the AF group compared to K, Kur and D groups (p <0.05). While the data obtained from AF + Kur group did not show any difference in creatinine level compared to the AF group (p> 0.05), it was found that the BUN level decreased,and the uric acid level was significantly lower (p <0.05). In conclusion, it was concluded that curcumin, which is known as a good antioxidant and anti-inflammatory system to heal liver and kidney damage, control inflammation, reduce oxidative stress and support the antioxidant defence system in rats with experimental chronic aflatoxicosis with AFB1 may be very useful in preventing or mitigating the harmful effects of AFB1 toxicity in terms of parameters examined.
Açıklama
Anahtar Kelimeler
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Hatipoğlu, D. (2021). Oral olarak aflatoksin B1 verilen ratlarda kurkuminin bazı karaciğer enzimleri, sitokinler ile böbrek fonksiyonları üzerine etkisi. (Doktora Tezi). Selçuk Üniversitesi, Sağlık Bilimleri Enstitüsü, Konya.
