The effects of statins on testicular ischemia reperfusion-induced histopathologic injury
Küçük Resim Yok
Tarih
2012
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Testiküler torsiyon infertiliteye neden olabilen yaygın bir sendromdur. Bu çalışma farklı farmakolojik özelliklere sahip statinlerin, testiküler torsiyon/detorsiyonu yansıtan bir model olan testiküler iskemi/reperfüzyon (IR) hasarı modelinde, tek dozlarının histopatolojik etkilerini değerlendirmek için planlandı. Gereç ve Yöntem: Sıçanlar operasyondan önce intraperitonal (i.p.) tiyopental sodyum (50 mg/kg) ile anestezi edildi. Sıçanların sol testiküler arter ve venleri 1 saat süreyle oklüze edildikten sonra, 24 saat reperfüze edildi. Histolojik değerlendirme için orşiektomi yapıldı. Simvastatin (5 mg/kg, i.p.), pravastatin (5 mg/kg, i.p.), atorvastatin (10 mg/kg, i.p.) veya çözücü (% 10 dimethilsülfoksit) reperfüzyondan önce uygulanıldı. Bulgular: IR sol testislerde belirgin histopatolojik hasara neden oldu. IR grubunun ortalama Johnsen skoru sham grubundan daha düşüktü (p0.001). Simvastatinle tedavi edilmiş testislerin skoru sham grubundan daha düşük (p0.001), fakat IR grubundan daha yüksek bulundu (p0.004). Atorvastatin ve pravastatin tedavileri ortalama Johnsen skorunu değiştirmedi. Sonuç: Bu çalışma, IR ile oluşturulan histopatolojik hasarı, tek doz uygulanan simvastatin tedavisinin azalttığını; tek doz atorvastatin ve pravastatin tedavilerinin ise değiştirmediğini göstermektedir. Sonuç olarak, statinlerin sergilemiş olduğu bu farklı etkilerin, onların farklı kimyasal ve farmakolojik özelliklerine bağlı olabileceğini düşünmekteyiz.
Objective: Testicular torsion is a common syndrome that could lead to infertility. This study was designed to evaluate the histopathological effects of single dose of statins which have different pharmacological properties, in testicular ischemia/ reperfusion (IR) injury model that resembles testicular torsion/detorsion. Material and Methods: Rats were anesthetized with intraperitoneal (i.p.) thiopental sodium (50 mg/kg) before the operation. Left testicular artery and vein of rats were occluded for 1 hour and the organ was allowed to reperfuse 24 hour before the orchiectomy for histologic examination. Simvastatin (5 mg/kg, i.p.), pravastatin (5 mg/kg, i.p.), atorvastatin (10 mg/kg, i.p.) or vehicle (10 % dimethylsulfoxide) was administrated before reperfusion. Results: IR caused a significant histopathologic damage of left testis. The mean Johnsen score in IR group was lower than sham group (p0.001). The score of testes treated with simvastatin was lower than sham (p0.001) but higher than IR group (p0.004). Atorvastatin and pravastatin treatments did not alter the mean Johnsen score. Conclusion: The present study showed that simvastatin treatment reduced IR-induced histopathologic injury, while atorvastatin and pravastatin did not alter. In conclusion, we thought that these different effects of statins may be dependent on their different chemical and pharmacological properties.
Objective: Testicular torsion is a common syndrome that could lead to infertility. This study was designed to evaluate the histopathological effects of single dose of statins which have different pharmacological properties, in testicular ischemia/ reperfusion (IR) injury model that resembles testicular torsion/detorsion. Material and Methods: Rats were anesthetized with intraperitoneal (i.p.) thiopental sodium (50 mg/kg) before the operation. Left testicular artery and vein of rats were occluded for 1 hour and the organ was allowed to reperfuse 24 hour before the orchiectomy for histologic examination. Simvastatin (5 mg/kg, i.p.), pravastatin (5 mg/kg, i.p.), atorvastatin (10 mg/kg, i.p.) or vehicle (10 % dimethylsulfoxide) was administrated before reperfusion. Results: IR caused a significant histopathologic damage of left testis. The mean Johnsen score in IR group was lower than sham group (p0.001). The score of testes treated with simvastatin was lower than sham (p0.001) but higher than IR group (p0.004). Atorvastatin and pravastatin treatments did not alter the mean Johnsen score. Conclusion: The present study showed that simvastatin treatment reduced IR-induced histopathologic injury, while atorvastatin and pravastatin did not alter. In conclusion, we thought that these different effects of statins may be dependent on their different chemical and pharmacological properties.
Açıklama
Anahtar Kelimeler
Cerrahi
Kaynak
Fırat Üniversitesi Sağlık Bilimleri Tıp Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
26
Sayı
1
