Bruselloz şüpheli olgularda Brucella canis seroprevalansının araştırılması
Küçük Resim Yok
Tarih
2013
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info:eu-repo/semantics/openAccess
Özet
Köpek brusellozunun etkeni olan Brucella canis insanlara bulaştığında diğer türlere göre daha hafif veya asemptomatik enfeksiyonlara neden olmaktadır. Etken insanlara ya hasta köpeklerden doğrudan temas ile ya da laboratuvarlarda çalışma esnasında bulaşmaktadır. B.canis enfeksiyonları hastanelerde rutin olarak araştırılmamaktadır. Ülkemizde insan popülasyonunda B.canis enfeksiyonlarının mevcut durumunu ortaya koyacak araştırmalar sınırlıdır. Bu çalışmada, bruselloz ön tanısı alan hastalarda B.canis seroprevalansının araştırılması amaçlanmıştır. Çalışmaya, Mart-Ağustos 2011 tarihleri arasında Konya Eğitim ve Araştırma Hastanesinin çeşitli poliklinik ve kliniklerinden brusellozis şüphesi ile tıbbi mikrobiyoloji laboratuvarına gönderilen, smooth Brucella antijeninin kullanıldığı Rose Bengal Plate Test (Seromed, Türkiye) sonucu negatif olan 652si kadın 1000 hastaya (yaş aralığı 15-65 yaş) ait serum örneği dahil edilmiştir. Hastalar, ateş, gece terlemesi, baş ağrısı, halsizlik, iştahsızlık, kas ve eklem ağrısı gibi bruselloz klinik belirtileriyle hastaneye başvuran olgulardır. B.canise karşı özgül antikorların saptanması amacıyla, B.canis NCTC 10854 suşundan R (rough) tipi antijen hazırlanmış ve hızlı lam aglütinasyon testi (LAT) ile modifiye plak aglütinasyon testi (MAT) uygulanmıştır. Hasta serumu örneklerinin %0.34 (34/1000)ü LAT antijeni ile iki dakika içerisinde aglütinasyon vermiş ve B.canis yönünden pozitif olarak kabul edilmiştir. Antikor titresinin belirlenmesi amacıyla yapılan MAT ile örneklerin 22 (%0.22)sinden pozitif sonuç alınmış; hasta serumlarının biri 1/48, beşi 1/96, altısı 1/192, altısı 1/384 ve dördü 1/768 titrede pozitif bulunmuştur. B.canis antikorları pozitif olan 22 hastanın 17si kadın, beşi erkek olup, cinsiyetler arasındaki farkın istatistiksel olarak anlamlı olduğu belirlenmiştir (p 0.05). Sonuç olarak, ülkemiz hastanelerinde, bruselloz için yapılan serolojik taramalarda S tipi brusella antijenlerinin yanı sıra R tipi antijenlerin de kullanılmasının, gerek tanı gerekse seroepidemiyolojik verilere katkıda bulunabileceği düşünülmüştür.
Brucella canis which is the main etiologic agent of brucellosis in dogs, can be transmitted to man. It causes mild or asymptomatic infection in human compared with other Brucella species. B.canis can be transmitted to man either by laboratory accidents or contact with infected dogs. Since B.canis infections in humans are not routinely investigated in hospitals in Turkey, the data are limited to reveal the current status of B.canis infections in people in our country. The purpose of this study was to determine the seroprevalence of B.canis infection in brucellosis-suspected cases. The study was conducted at Konya Education and Research Hospital, (located at Central Anatolia of Turkey) during March-August 2010 period. Serum samples were obtained from 1000 patients (age range: 15-65 years; 652 of them were women) presented with brucellosis-like symptoms, including fever, headache, night sweats, appetite loss, weakness, arthralgia and myalgia. Rose Bengal Plate Tests (Seromed, Turkey) for smooth Brucella species were negative in all serum samples. Rough type B.canis antigen was prepared with B.canis NCTC 10854 strain for serodiagnosis. Antibody responses to B.canis in the serum samples were investigated by rapid slide agglutination test (SAT) and modified plate agglutination test (MPAT). Of the 1000 sera tested, 34 (0.34%) were found to be positive with SAT while the remaining were found negative. MPAT was used for the detection of antibody titer and 22 (0.22%) out of 1000 sera were found positive with MPAT (one had 1/48, five had 1/96, six had 1/192, six had 1/384, four had 1/768 titers). Among 22 positive patients, 17 were female and five were male, and the difference between the genders was found statistically significant (p< 0.05). It was concluded the use of both S and R antigens in the serological tests applied for the diagnosis of brucellosis in our country will supplement both diagnosis and seroepidemiological data related to brucellosis.
Brucella canis which is the main etiologic agent of brucellosis in dogs, can be transmitted to man. It causes mild or asymptomatic infection in human compared with other Brucella species. B.canis can be transmitted to man either by laboratory accidents or contact with infected dogs. Since B.canis infections in humans are not routinely investigated in hospitals in Turkey, the data are limited to reveal the current status of B.canis infections in people in our country. The purpose of this study was to determine the seroprevalence of B.canis infection in brucellosis-suspected cases. The study was conducted at Konya Education and Research Hospital, (located at Central Anatolia of Turkey) during March-August 2010 period. Serum samples were obtained from 1000 patients (age range: 15-65 years; 652 of them were women) presented with brucellosis-like symptoms, including fever, headache, night sweats, appetite loss, weakness, arthralgia and myalgia. Rose Bengal Plate Tests (Seromed, Turkey) for smooth Brucella species were negative in all serum samples. Rough type B.canis antigen was prepared with B.canis NCTC 10854 strain for serodiagnosis. Antibody responses to B.canis in the serum samples were investigated by rapid slide agglutination test (SAT) and modified plate agglutination test (MPAT). Of the 1000 sera tested, 34 (0.34%) were found to be positive with SAT while the remaining were found negative. MPAT was used for the detection of antibody titer and 22 (0.22%) out of 1000 sera were found positive with MPAT (one had 1/48, five had 1/96, six had 1/192, six had 1/384, four had 1/768 titers). Among 22 positive patients, 17 were female and five were male, and the difference between the genders was found statistically significant (p< 0.05). It was concluded the use of both S and R antigens in the serological tests applied for the diagnosis of brucellosis in our country will supplement both diagnosis and seroepidemiological data related to brucellosis.
Açıklama
Anahtar Kelimeler
Mikrobiyoloji
Kaynak
Mikrobiyoloji Bülteni
WoS Q Değeri
Scopus Q Değeri
Cilt
47
Sayı
1
