HIGH FREQUENCY REGENERATION OF CRITICALLY ENDANGERED ENDEMIC PLANT CENTAUREA LYCAONICA

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dc.contributor.authorAtalay, Emine
dc.contributor.authorErisen, Semiha
dc.date.accessioned2020-03-26T19:41:50Z
dc.date.available2020-03-26T19:41:50Z
dc.date.issued2017
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractCentaurea lycaonica is a critically endangered endemic plant species found in Turkey. In this study in vitro culture techniques were evaluated as an ex situ strategy for conservation of this species. Achenes were surface sterilized and cultured either in MS medium alone or along with GA(3) in an attempt to induce germination. It was observed that the overall germination rate was low and that the highest rate (20%) was obtained when hormone-free MS medium was used. For axillary shoot pro-pagation, nodal explants were cultured on MS me-dium supplemented with BAP, Kin or TDZ. The highest shoot number (8.7 shoots per explant) was obtained on MS medium supplemented with 0.5 mg L BAP. Leaf explants were removed from the in vitro axillary shoots that were used as explants and cultured on medium including BAP, Kin, TDZ alone or with NAA. The calli obtained exhibited differences in morphology which appeared to be dependent on type and combination of PGR used. It was found that BAP was the only PGR that was capable of eliciting a positive response with respect to shoot regeneration. The highest shoot number (32.3 shoots per explant) was obtained on MS medium supplemented with 0.5 mg L-1 BAP and 0.2 mg L-1 NAA. Regenerated shoots were transferred into various rooting media. The best rooting rate (90%) was obtained on MS medium supplemented with 2 mg L-I IAA. Rooted shoots were successfully acclimatized and were observed to grow vigorously under ex vitro conditions.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TOVAG-112 O 492]en_US
dc.description.sponsorshipThis research was supported by The Scientific and Technological Research Council of Turkey (TUBITAK) Project "In vitro Prolification of Critical Endemic Centaurea lycaonica Context of Ex-situ Conservation", Project Number: TOVAG-112 O 492.en_US
dc.identifier.endpage6363en_US
dc.identifier.issn1018-4619en_US
dc.identifier.issn1610-2304en_US
dc.identifier.issue11en_US
dc.identifier.startpage6356en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/35171
dc.identifier.volume26en_US
dc.identifier.wosWOS:000417007300007en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherPARLAR SCIENTIFIC PUBLICATIONS (P S P)en_US
dc.relation.ispartofFRESENIUS ENVIRONMENTAL BULLETINen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectEndemicen_US
dc.subjectgerminationen_US
dc.subjectmicropropagationen_US
dc.subjectorganogenesisen_US
dc.subjectC. lycaonicaen_US
dc.titleHIGH FREQUENCY REGENERATION OF CRITICALLY ENDANGERED ENDEMIC PLANT CENTAUREA LYCAONICAen_US
dc.typeArticleen_US

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