Deneysel akut organik fosfor toksisitesi tedavisine eklenen e vitaminin olumlu etkileri
Küçük Resim Yok
Tarih
2011
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Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
AmaçPestisit zehirlenmelerinde reaktif oksijen türevlerinin artmış üretimine bağlı olarak oksidatif stres geliştiği bildirilmiştir. Doku ROS seviyeleri doku hasarının en önemli göstergelerindendir. Bu çalışmada, akut organofosfat (OF) zehirlenmesinde ek olarak kullanılacak E vitamininin tedavisinin kandaki ve karaciğer dokusundaki kolin esteraz (KE) ve malondialdehit (MDA) düzeyleri üzerine etkilerini araştırmak ve sadece antidot tedavisi verilen grup ile karşılaştırarak OF zehirlenmesi tedavisinde kullanılıp kullanılamayacağını belirlemektir.Gereç ve YöntemÇalışmada 20 Yeni Zelanda cinsi tavşan randomize olarak sham (n8), pralidoksim (PAM)atropin (n6) ve E vitamini (n6) olarak 3 gruba ayrıldı. Her denekten toksisite öncesi plazma KE, serum ve eritrosit MDA değerlerini ölçmek için kan örnekleri alındıktan sonra orogastrik yoldan 50 mg/kg 2.2-diklorvinil dimetil fosfat verildi. PAMatropin grubundaki deneklere 30 mg/kg IV bolus, ardından 15 mg/kg PAM ve 0.05 mg/kg atropin her 4 saatte İV verildi. E vitamini grubundaki deneklere benzer atropin ve PAM tedavisine ilaveten 250 mg/kg E vitamini tek doz İM uygulandı. Deneklerden tedavi başlatıldıktan sonra 12. ve 24. saatlerde kan örnekleri alındı. Tüm deneklerden aynı parametreleri değerlendirmek üzere karaciğer dokusu örnekleri alındı. Denekler yüksek dozda İV anestezik verilerek sakrifiye edildiler. BulgularE vitamini grubunun eritrosit MDA’sı PAMatropin grubundan anlamlı düşük (p0.003) tespit edildi. E vitamini grubunun karaciğer dokusundaki KE düzeyi PAMatropin grubundan anlamlı olarak yüksekti (p0.001). E vitamini grubundaki tavşanların karaciğer doku MDA’sı PAM-atropin grubundan anlamlı olarak düşüktü (p0.001). SonuçAkut OF zehirlenmesinde antidot tedavisine eklenen E vitamininin hem eritrosit ve karaciğer dokusu lipid peroksidasyonu üzerine hem de karaciğer dokusu KE aktivitesi üzerine iyileştirici etkisi vardır.
ObjectivesOxidative stress by increased production of reactive oxygen species has been implicated in the toxicity of many pesticides. The tissue levels of ROS are one of the most important indicator of tissue injury. The aim of this study was to examine the effects of vitamin E treatment in acute organophosphate poisoning (AOP) on choline esterase (CE) and Malondialdehit (MDA) levels in the liver tissue and blood and to compare with antidote treatment.MethodsTwenty New Zaeland type rabbits were divided into randomly three groups as sham (n8), pralidoxime (PAM)atropine (n6), and vitamin E (n6). blood samples were taken from each test subjects to measure plasma CE, serum and erythrocyte MDA values before toxicity. 50 mg/kg 2,2-dichlorovinyl dimethyl phosphate were given to all subjects orogastrically. The PAM-atropine group were given 30 mg/kg IV bolus, then 15 mg/kg PAM and 0.05 mg/kg atropine IV every 4 hours. The vitamin E group received 250 mg/kg vitamin E single dose IM in addition to same atropine and PAM treatment. Blood samples were obtained from the all subjects in the 12th and 24th hours followed by the initiation of treatment. The liver tissue samples were obtained to evaluate in order to evaluate same parameters. The test subjects were sacrificed by high dose IV anesthesia.ResultsThe erythrocyte MDA of vitamin E group was significantly lower than PAM-atropine group (p0.003). Liver tissue CE level of vitamin E group was considerably higher than PAM-atropine group (p<0.001). Liver tissue MDA of vitamin E group was significantly lower than PAM-atropine group (p<0.001). ConclusionsIncluded in the treatment of acute AOF toxicity, vitamin E has a curative effect on both erythrocyte and liver tissue lipid peroxidation and tissue CE activity.
ObjectivesOxidative stress by increased production of reactive oxygen species has been implicated in the toxicity of many pesticides. The tissue levels of ROS are one of the most important indicator of tissue injury. The aim of this study was to examine the effects of vitamin E treatment in acute organophosphate poisoning (AOP) on choline esterase (CE) and Malondialdehit (MDA) levels in the liver tissue and blood and to compare with antidote treatment.MethodsTwenty New Zaeland type rabbits were divided into randomly three groups as sham (n8), pralidoxime (PAM)atropine (n6), and vitamin E (n6). blood samples were taken from each test subjects to measure plasma CE, serum and erythrocyte MDA values before toxicity. 50 mg/kg 2,2-dichlorovinyl dimethyl phosphate were given to all subjects orogastrically. The PAM-atropine group were given 30 mg/kg IV bolus, then 15 mg/kg PAM and 0.05 mg/kg atropine IV every 4 hours. The vitamin E group received 250 mg/kg vitamin E single dose IM in addition to same atropine and PAM treatment. Blood samples were obtained from the all subjects in the 12th and 24th hours followed by the initiation of treatment. The liver tissue samples were obtained to evaluate in order to evaluate same parameters. The test subjects were sacrificed by high dose IV anesthesia.ResultsThe erythrocyte MDA of vitamin E group was significantly lower than PAM-atropine group (p0.003). Liver tissue CE level of vitamin E group was considerably higher than PAM-atropine group (p<0.001). Liver tissue MDA of vitamin E group was significantly lower than PAM-atropine group (p<0.001). ConclusionsIncluded in the treatment of acute AOF toxicity, vitamin E has a curative effect on both erythrocyte and liver tissue lipid peroxidation and tissue CE activity.
Açıklama
Anahtar Kelimeler
Cerrahi
Kaynak
Türkiye Acil Tıp Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
11
Sayı
2
