Phenotypic and molecular identification of extended-spectrum beta-lactamases in Escherichia coli in healthy broilers at the west region of Iran
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Date
2018
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Publisher
Selçuk Üniversitesi Veterinerlik Fakültesi
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info:eu-repo/semantics/openAccess
Abstract
Amaç: İnsana yayılma olasılığı yüksek olduğu için kümes hayvanlarında genişlemiş spektrumlu beta-laktamaz (GSBL) genlerinin fekal taşıyıcılarının varlığı endişe verici bir durumdur. Bu çalışma, İran'ın batısında sağlıklı broyler bağırsağından izole edilen Escherichia coli'deki ESBL taşıyıcılarının sıklığını tahmin etmek için yapılmıştır. Gereç ve Yöntem: Görünüş olarak sağlıklı broilerden toplam 300 dışkı örneği toplandı. E. coli suşları izole edildi, moleküler olarak doğrulandı ve disk kombinasyon yöntemi, antimikrobiyal duyarlılık testi ve ESBL-saptama polimeraz zincir reaksiyonları (PZR) kullanılarak incelendi. Bulgular: 285 E. coli suşu, biyokimyasal olarak izole edildi ve hepsi, türe özgü bir PZR reaksiyonuyla doğrulandı. Fenotipik yöntemle saptanan 156 ESBL üreten E. coli üzerinde antimikrobiyal duyarlılık testi, bunların % 100'ünün en az bir antibiyotiğe dirençli olduğunu, bunların hepsinin de imipeneme duyarlı olduğunu ortaya koymuştur. BlaTEM, blaCTX-M ve blaSHV genlerinin prevalansı sırasıyla% 52.63,% 38.59 ve% 30.17 idi. Birkaç ESBL geninin eşzamanlı varlığı, izolatların % 52.61'inde tespit edilmiştir. Öneri: Kümes hayvanlarında çoklu dirençli ESBL taşıyıcılarının yaygınlığı, halk sağlığına yönelik bir tehdittir. Bölgedeki kümes hayvanları endüstrisinde tutarlı izleme ve uygun antibiyotik kullanımı uygulanmalıdır.
Aim: The presence of fecal carriers of extended-spectrum beta-lactamase (ESBL) genes in poultry is worrisome because it is likely to spread to a human being. This study was proposed to estimate the frequency of ESBLs carriers in Escherichia coli isolated from healthy broiler’s gut in west of Iran. Materials and Methods: A total of 300 fecal samples were collected from apparently healthy broiler. E. coli strains were isolated, confirmed molecularly and studied using disk combination method, antimicrobial susceptibility test and ESBL-detection polymerase chain reactions (PCR). Results: Two hundred and eighty five strains of E. coli were isolated biochemically and all were confirmed in a speciesspecific PCR reaction. Antimicrobial susceptibility test on 156 ESBL-producing E. coli identified by phenotypic method revealed that 100 % of them were resistant to at least one antibiotic, while all were sensitive to imipenem. The prevalence of blaTEM, blaCTX-M, and blaSHV genes was 52.63%, 38.59%, and 30.17% respectively. The simultaneous existence of several ESBL genes was identified in 52.61% of the isolates. Conclusions: The vast prevalence of ESBL carriers with multiple resistance in poultry is a threat to public health. Consistent monitoring and proper use of antibiotics in the region’s poultry industry should be applied.
Aim: The presence of fecal carriers of extended-spectrum beta-lactamase (ESBL) genes in poultry is worrisome because it is likely to spread to a human being. This study was proposed to estimate the frequency of ESBLs carriers in Escherichia coli isolated from healthy broiler’s gut in west of Iran. Materials and Methods: A total of 300 fecal samples were collected from apparently healthy broiler. E. coli strains were isolated, confirmed molecularly and studied using disk combination method, antimicrobial susceptibility test and ESBL-detection polymerase chain reactions (PCR). Results: Two hundred and eighty five strains of E. coli were isolated biochemically and all were confirmed in a speciesspecific PCR reaction. Antimicrobial susceptibility test on 156 ESBL-producing E. coli identified by phenotypic method revealed that 100 % of them were resistant to at least one antibiotic, while all were sensitive to imipenem. The prevalence of blaTEM, blaCTX-M, and blaSHV genes was 52.63%, 38.59%, and 30.17% respectively. The simultaneous existence of several ESBL genes was identified in 52.61% of the isolates. Conclusions: The vast prevalence of ESBL carriers with multiple resistance in poultry is a threat to public health. Consistent monitoring and proper use of antibiotics in the region’s poultry industry should be applied.
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Keywords
Antibiyotik direnci, ESBL üreten Escherichia coli, İran, Antibiotic resistance, ESBL-producing Escherichia coli, Iran
Journal or Series
Eurasian Journal of Veterinary Sciences
WoS Q Value
Scopus Q Value
Volume
34
Issue
3
Citation
Sheikhi S., Ahmadi E., (2018). İran’ın batı bölgesinde sağlıklı broylerlerdeki Escherichia coli’lerde geniş spektrumlu beta laktamazların fenotipik ve moleküler identifikasyonu. Eurasian Journal of Veterinary Sciences, 34 (3), 178-184. DOI: 10.15312/EurasianJVetSci.2018.198
