Isolation and molecular identification of thermophilic Campylobacter species from mallard (Anas platyrhynchos)
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Dosyalar
Tarih
2019
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi Veteriner Fakültesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Öz Amaç: Bu çalışmada, yaban ördeklerinde insan ve hayvan sağlığını olumsuz yönde etkileyen Campylobacter türlerinin araştırılması amaçlandı. Gereç ve Yöntem: Yaban ördeklerinden toplanan 110 dışkı örneği kültürel ve moleküler yöntemlerle incelendi. Ön zenginleştirme amacıyla örnekler Preston Campylobacter Enrichment Broth’a ekildi ve inkübe edildi. İnkübasyondan sonra ön zenginleştirme kültürden Preston Campylobacter Selective Agar’a ekim yapıldı ve 42°C 24-48 saat inkübasyona bırakıldı. Üremenin olduğu kültürler önce koloni morfolojisi ve sonra mikroskobik görünüm açısından değerlendirildi. Şüpheli koloniler değerlendirildikten sonra multiplex PCR ile incelendi. Bulgular: Toplam 110 dışkı örneğinin 10'unda (%9,1) Campylobacter spp. bulundu. Yapılan Multiplex PCR ile 10 izolatın Campylobacter jejuni olduğu teyit edilmiştir. Öneri: Bu çalışma ile, yaban ördeklerinden Campylobacter spp. varlığı belirlendi ve yaban ördeklerinin Campylobacter türleri için önemli bir rezervuar olabileceği kanaatine varıldı. Ayrıca, Kars bölgesi kuşların göç güzergahında olduğu için göçmen kuşlarının potansiyel taşıyıcı olabilecekleri göz önünde tutulmalıdır.
Aim: The aim of this study was to investigate the Camplyobacter species which adversely affect human and animal health in mallard. Materials and Methods: 110 stool samples were collected from the mallard and examined by the cultural and molecular method. For pre-enrichment step, samples were inoculated with Preston Campylobacter Enrichment Broth. At the end of the incubation, preenriched culture was inoculated on Preston Campylobacter Selective Agar and the plates were incubated for 48-72 hours at 42°C. The cultures in which the growth was observed were first evaluated for the colony morphology and then for microscopic appearance. Suspected colonies were examined with multiplex PCR. Results: In this study, Campylobacter spp. was found in 10 (9.1%) of the 110 stool specimens. All 10 isolates were typed as Campylobacter jejuni by multiplex PCR. Conclusion: In this study, Campylobacter was detected in mallards and it was concluded that mallard may be an important reservoir for Campylobacter species. Besides, since Kars area is on the migration route of birds, it is important to examine the migratory birds and to determine the infections which birds have the potential.
Aim: The aim of this study was to investigate the Camplyobacter species which adversely affect human and animal health in mallard. Materials and Methods: 110 stool samples were collected from the mallard and examined by the cultural and molecular method. For pre-enrichment step, samples were inoculated with Preston Campylobacter Enrichment Broth. At the end of the incubation, preenriched culture was inoculated on Preston Campylobacter Selective Agar and the plates were incubated for 48-72 hours at 42°C. The cultures in which the growth was observed were first evaluated for the colony morphology and then for microscopic appearance. Suspected colonies were examined with multiplex PCR. Results: In this study, Campylobacter spp. was found in 10 (9.1%) of the 110 stool specimens. All 10 isolates were typed as Campylobacter jejuni by multiplex PCR. Conclusion: In this study, Campylobacter was detected in mallards and it was concluded that mallard may be an important reservoir for Campylobacter species. Besides, since Kars area is on the migration route of birds, it is important to examine the migratory birds and to determine the infections which birds have the potential.
Açıklama
Anahtar Kelimeler
Termofilik Campylobacter, izolasyon, multiplexPCR, yaban ördeği, isolation, multiplex-PCR, mallard
Kaynak
Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
35
Sayı
4
Künye
Sağlam A. G., Çelik E., Coşkun M.R., Çelebi Ö., Büyük F.., Şahin M., Otlu S., (2019). Isolation and molecular identification of thermophilic Campylobacter species from mallard (Anas platyrhynchos). Eurasian J Vet Sci, 2019, 35, 4, 199-203
DOI: 10.15312/EurasianJVetSci.2019.243
