Deneysel kombine kafa travması ve hemorajik şok modelinde sıvı resusitasyonu ve antioksidan tedavinin beyin doku laktik asidozu ve lipid peroksidasyonu üzerine etkileri
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Dosyalar
Tarih
2006
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi Tıp Fakültesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada, ratlarda deneysel olarak oluşturulan kafa travması ve hemorajik şok modelinde sıvı resusitasyonu ve DMSO'nun doku laktik asidozu ve lipid peroksidasyonu üzerine etkisi araştırıldı. Denekler 5 ayrı gruba ayrıldı. İlk 2 grup (n=8) sham ve kontrol grubunu, son 3 grup (n=7) ise tedavi verilen grubu oluşturdu. Deneklere Marmarou metoduyla kafa travması ve ayrıca hemorajik şok oluşturuldu. 3.grupda OAB 60 mmHg, 4.grupta OAB 80 mmHg olacak sekilde sadece sıvı replasman tedavisi, 5. grupta ise OAB 80 mmHg olacak şekilde sıvı replasmanına ek olarak antioksidan tedavi amacıyla DMSO verildi. Laktik asidoz ve lipid peroksidasyon derecesi, beyin dokusunda laktat ve MDA seviyelerinin ölçülmesi ile değerlendirildi. Beyin dokusu MDA değerleri, 2., 3. ve 4. gruplarda, sham grubuna göre belirgin olarak artmış olarak tespit edilmesine rağmen (P< 0,05), antioksidan tedavi verilen 5. grupta bu oranda bir artış tespit edilmedi. Doku laktat düzeyleri ise tedavi verilmeyen 2.grup ve OAB'ın 60 mmHg civarında tutulduğu 3. grupta, sham grubuna göre belirgin (P< 0,05) olarak artmışken, OAB'ın 80 mmHg civarında tutulduğu 4. ve 5. grupta istatistiksel olarak anlamlı bir artış saptanmadı Sonuç olarak; ratlarda deneysel kombine kafa travması ve hemorajik şok modelinde erken dönemde uygun sıvı tedavisi ile birlikte verilen DMSO'nun daha yüksek ortalama arteryel basınç değerlerinde doku laktik asidozu ve lipid peroksidasyonu üzerine etkili olduğu bulundu.
In the present study, the effects of volume replacement and DMSO on brain tissue lactate and malondialdehyde (MDA) levels after combined experimental hemorrhagic shock and traumatic brain injury in rats was studied. Rats were assigned to five groups. First two of five(n=8,each) were constituted as sham and control, the others were treatment groups(n=7,each). Except sham group, experimental hemorrhagic shock and traumatic brain injury according to Marmarou?s method were performed. No treatment was given to control group. Volume replacement was administered to maintain a mean arterial blood pressure (MAP) of >60 mmHg in the third group and of >80 mmHg in the fourth and fifth groups. In addition to volume replacement , DMSO was given to the fifth groups as an anti-oksidant treatment. The degree of lactic asidozis and lipid peroxidation was evaluated by measuring the levels of lactate and MDA of damaged brain tissue. Although there was a markedly increase of MDA levels in the second, third and the fourth groups (P< 0,05), no significant differences were found in the fifth, according to sham group. And the lactate levels, This therapy resulted in substantially higher lactate levels in the third group (MAP of >60 mmHg) and untreated group than those of groups of rats that had been treated with 9 â ° physiological saline solution at higher MAP(of >80 mmHg) (P< 0,05). As a result, we concluded that DMSO and convenient volume replacement therapy at higher MAP is effective on lactic asidosis and lipid peroxidation during the acute phase of combined experimental hemorrhagic shock and head trauma model in rats.
In the present study, the effects of volume replacement and DMSO on brain tissue lactate and malondialdehyde (MDA) levels after combined experimental hemorrhagic shock and traumatic brain injury in rats was studied. Rats were assigned to five groups. First two of five(n=8,each) were constituted as sham and control, the others were treatment groups(n=7,each). Except sham group, experimental hemorrhagic shock and traumatic brain injury according to Marmarou?s method were performed. No treatment was given to control group. Volume replacement was administered to maintain a mean arterial blood pressure (MAP) of >60 mmHg in the third group and of >80 mmHg in the fourth and fifth groups. In addition to volume replacement , DMSO was given to the fifth groups as an anti-oksidant treatment. The degree of lactic asidozis and lipid peroxidation was evaluated by measuring the levels of lactate and MDA of damaged brain tissue. Although there was a markedly increase of MDA levels in the second, third and the fourth groups (P< 0,05), no significant differences were found in the fifth, according to sham group. And the lactate levels, This therapy resulted in substantially higher lactate levels in the third group (MAP of >60 mmHg) and untreated group than those of groups of rats that had been treated with 9 â ° physiological saline solution at higher MAP(of >80 mmHg) (P< 0,05). As a result, we concluded that DMSO and convenient volume replacement therapy at higher MAP is effective on lactic asidosis and lipid peroxidation during the acute phase of combined experimental hemorrhagic shock and head trauma model in rats.
Açıklama
Anahtar Kelimeler
Kafa travması, Head trauma, Hemorajik şok, Hemorrhagic shock, Laktat, Lactate
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Köylü, R. (2006). Deneysel kombine kafa travması ve hemorajik şok modelinde sıvı resusitasyonu ve antioksidan tedavinin beyin doku laktik asidozu ve lipid peroksidasyonu üzerine etkileri. Selçuk Üniversitesi, Yayımlanmış uzmanlık tezi, Konya.
