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  • Küçük Resim
    Öğe
    Aflatoksin ve Aflatoksin Bağlayıcısı Olan Polivinilpolipirolidon (PVPP) Verilen Broilerlerde Peritoneal Makrofajların Fagositik ve Mikrobisidal Aktivitelerinin Belirlenmesi
    (Selçuk Üniversitesi, 1996) Çelik, İlhami; Demet, Ömer; Oğuz, Halis; Boydak, Murat; Dönmez, Hasan Hüseyin
    In thıs study effects of aflatoxlcosis. which was experımentally induced by giving mixed aflatoxins ın feed (2. 5 mg/kg) for 21 days. on phagocytic and Candidacidal activities of chicken peritoneal macrophages and pre· ventıve actıon ol polyvinylpolypyrrolidone (PVPP) supplementation (3g/kg) were investigated. Feeding experıments were performed on totally 200 mala one·day·old chlcks of Avlan strain. The animals were divided into tour groups each containing 50 chickens and leed with dilterant diets as fallows: First group, with commercial broıler feed. Second group, wıth mixed aflatoxıns containing (2.5 mg/kg) commercial broiler feed Third group, wıth PVPP (0.3 %) supplemented commercial broiler feed. Fourth group, with both PVPP (0.3 %) and mixed aflatoxıns (2.5 mgfkg) con· taınıng feed At the end ol the feeding experiment, peritoneal macrophages were isolated. The phagocytıc and ınt· racellular Candidacidal indlces were determined. The data were analysed statistically. The results have revealed that allatoxıcosıs depressed both phagocytıc and Candidacidal potential of paritonaat macrophages. PVPP addrtıon pre· vented the anımals from deppressive effects caused by aflatoxins on peritoneal macrophage functıons.
  • Küçük Resim
    Öğe
    Akkaraman koyunlarının hemal düğümlerinin histolojisi ve alfa-naftil esetat esteraz (ANAE) pozitif lenfositlerin yerleşimleri üzerinde ışık mikroskopik bir çalışma
    (2005) Sur, Emrah; Aydın, Mehmet Faruk; Çelik, İlhami
    Bu çalışmada, Akkaraman koyunlarındaki hemal düğümlerin histolojik yapıları ve bu organlardaki alfa-naftil asetat es-teraz (ANAE) pozitif lenfositlerin yerleşimlerinin belirlenmesi amaçlandı. Bu amaçla 2 yaşlı 20 Akkaraman koyunundan alınan hemal düğümü örnekleri materyal olarak kullanıldı. Rutin histolojik işlemleri takiben alınan kesitlere Crossman'ın üçlü boyası, hematoksilen-eozin, Gordon ve Sweet'in retiküler iplik boyası, toluidin mavisi, metil-green pironin ve Pappenheim'ın panoptik boyama yöntemleri uygulanırken, kriyostat kesitlerinde alfa-naftil asetat esteraz (ANAE) demonstrasyonu gerçekleştirildi. Hemal düğümlerin düz kas hücrelerini de içeren bağ dokusundan bir kapsülle çevrildiği ve organın, içleri kanla dolu subkapsüler ve derin sinuslardan oluşan gelişmiş bir sinus sistemine sahip olduğu gözlendi. Hemal düğümlerin çatısını retiküler bağ dokusu oluşturmaktaydı. Organda korteks ve medula ayırımı belirgin değildi. Lenf folikülleri ve lenfatik kordonlarda plazma hücreleri, megakaryositler, makrofajlar ve mast hücreleri gözlendi. Organda belirgin lenf damarları ve lenfatik sinuslara rastlanmadı. ANAE-pozitif lenfositlerin, özellikle sekonder foliküllerin germinal merkezlerinde yoğunlaştığı dikkati çekti.
  • Küçük Resim Yok
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    Akut Submaksimal Egzersizin Immün Sisteme Etkileri
    (1999) Özdengül, Faik; Uysal, Hüseyin; Gökbel, Hakkı; Çelik, İlhami; Altındiş, Mustafa
    Amaç: Bu çalışma akut submaksimal egzersizin immün sistem üzerine etkilerini araştırmak için yapıldı. Yöntem: Yaşları 25-30 yıl arasında değişen 19 sağlıklı, sedanter erkek çalışmaya dahil edildi. Deneklere maksimum oksijen volümlerinin % 60'ına denk gelen kalp hızında 60 dakika bisiklet ergometresi ile egzersiz yaptırıldı. Egzersiz öncesinde ve hemen bitiminde alınan kan örneklerinde CD4 ve CD8 hücre sayısı ve serum immünglobulinleri çalışıldı. Bulgular: Egzersizle lenfosit, lökosit ve granülosit değerlerinde artma, CD4 hücrelerde azalma bulundu. IgG, IgM, IgA ve CD8 hücre değerlerinde kayda değer bir değişiklik bulunmadı. Sonuç: VO2max'ın % 60'ında yapılan 60 dakikalık bir egzersizin immün sistemi deprese edebileceği kanaatine varıldı.
  • Küçük Resim Yok
    Öğe
    Ankara tavşanı perifer kan lenfositlerinnin alfa- naftil asetat esteraz (ANAE) aktivitesi ve bazı AgNOR parametreleri üzerinde ışık mikroskobik çalışmalar
    (2007) Aydın, Mehmet Faruk; Öznurlu, Yasemin; Çelik, İlhami; Telatar, Tuğba; Sur, Emrah
    Bu çalışmada, Ankara tavşanının perifer kan lenfositlerinin (PBL) oranı, alfa-naftil asetat esteraz (ANAE) enzimi aktivitesi ve bazı AgNOR parametrelerinin belirlenmesi amaçlanmıştır. Çalışmada 8 adet erkek ve 8 adet dişi olmak üzere toplam 16 adet Ankara tavşanından alman perifer kan örneği kullanıldı. PBL oranlan dişilerde %73.25, erkeklerde ise %71.13 olarak tespit edildi. ANAE-pozitif lenfosit oranlan dişi tavşanlarda % 34.25, erkeklerde %34.63 olarak bulundu. Ortalama AgNOR sayısı dişilerde çekirdek başına 2.03, erkeklerde 2.05 olup, AgNOR alanı dişilerde 2.25 um2, erkeklerde ise 1.95 um2, çekirdek alanı dişilerde 11.28 \mu2 iken erkeklerde 11.24 \mu2, nispi AgNOR alanı dişilerde %19.96, erkeklerdeyse % 16.90 olarak tespit edildi. Grupların PBL'lerinin incelenen parametreleri arasında istatistiksel öneme sahip fark tespit edilmedi (P0.05).
  • Küçük Resim Yok
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    Broiler piliçlerde tibia'nın embriyonik gelişimi ve ossifikasyon süreci üzerinde ışık mikroskopik incelemeler
    (2007) Öznurlu, Yasemin; Çelik, İlhami
    Bu çalışmada, broilerlerde tibia'nın embriyonik gelişimi ve ossifikasyonu ışık mikroskobik düzeyde incelendi. Çalışmada, Hubbard ırkı broiler damızlıklara ait toplam 74 adet kuluçkalık yumurfa kullanıldı. Yumurtalar optimal koşullarda 21 gün süreyle inkübe edildi. İnkübasyonun 5, 7, 9, 11, 13, 15, 17, 19 ve 21. günlerinde açılan yumurtalardan çıkan embriyoların tibiaları yumuşak dokularından temizlendikten sonra, rutin histolojik yöntemlerle işlenerek parafinde bloklandı ve bloklardan alman 6 \mum kalınlığındaki kesitler Crossmon'un üçlü boyası, hematoksilen-eozin, Schmorl'un Pikro-tiyonin, Alizarin red-S ve Von Kossa metoduyla boyandı. Yapılan mikroskobik incelemelerde, kuluçkanın 5. gününde tibia'nm hiyalin kıkırdak modelinde ossifikasyön odaklarına rastlanmadı. Ossifikasyon odakları, yedinci ve dokuzuncu günlerde subperiostal lokalizasyonlu, dar bandlar halinde diyafizin orta bölgesinde gözlendi. On birinci günde, manşon biçimi alan mineralize sahadan merkezi bölgedeki kıkırdağa doğru vasküler invazyonun başlamasıyla birlikte, kıkırdak dokusunda da yıkımlanmanın başladığı tespit edildi. Sonuç olarak, broilerlerde tibia' nın hiyalin kıkırdak modelinde ossifikasyomm kuluçkanın yedinci gününde, diyafizin ortasındaki subperiostal mineralizasyon odakları halinde başladığı ve embriyonik dönem boyunca ilerleyerek decvam ettiği; distal ve proksimal bölgedeki konik kıkırdak kitlelerin kuluçka dönemi boyunca varlıklarım sürdürdükleri saptanmıştır.
  • Küçük Resim
    Öğe
    Broylerlerde Yağlı Karaci?ğer Sendromu Üzeri?nde Hi?stoloji?k ve Bi?yoki?myasal Çalışmalar
    (Selçuk Üniversitesi, 1987 Ocak) Aştı, Reşat; Tuncer, Şakir Doğan; Coşkun, Behiç; Başpınar, Nuri; Çelik, İlhami; Kalaycıoğlu, Leyla
    This study was carried out to investigate the correlation between the fatty liver syndrome and some blood values in broilers fed with rations containing corn, sunflower oil and animal fat. Totally 150 day-old Arbro - 7 female chicks were used in the experiment. There were three groups each containing 50 chicks. The experiment lasted in 56 days. At the end of the experiment, a severe case of fatty livers was observed in the group fed with corn based ration. The highest SGOT (P<0.05) and FFA values were found in this group. In the other two groups fed differently with ration containing sunflower oil and the animal fat, the lesser amount of lipid infiltrations were observed. The total serum cholesterol levels in the group fed with animal fat were higher than the other groups (P<0.01). As a result, it can be deduced that the determination of SGOT values as well as the histological investigation would be helpfull in the diagnosis of subclinical fatty liver syndrome.
  • Küçük Resim
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    Buğday, Mısır, Pi?ri?nç ve Yerfıstığında Aflatoksi?n Üreti?lmesi?
    (Selçuk Üniversitesi, 1995 Nisan) Demet, Ömer; Oğuz, Halis; Çelik, İlhami; Adıgüzel, Hasan
    In this study, the production of aflatoxin at a grade appropriate for dietary aflatoxin feeding studies were performed under our laboratory conditions. After sporulation Aspergillus parasiticus NRRL 2999 strain in the tubes containing Patato Dextrose Agar (Merck), the spores were removed by washings with 0.005% Triton X-100 (Merck) and an enriched suspensions of spores were obtained, following determination of the spore numbers in per ml of sus- pension, the suspension was used the inoculate the sterilized substrate (wheat, corn, rice and peanut) in erlenmeyer flasks and after inoculation the flasks were into an incubator for fermentation at 28 °C. Toplam twelve erlenmeyer flasks each having three ones for each substrate were fermented. At the end of the five days fermentation aflatoxin levels of the flasks were the determined by Fluorescence Spectrophotometry. Aflatoxin levels were measured as mg/ kg substrate (ppm) as follows: 71.88 for wheat (72.55% B1. 11.31 % B2, 11.28% G, and 4.86 % G?); 48.14 for corn (78.84 % B1, 7.45 % B2, 10.85% G1 and 2.86% G2); 63.64 for rice (83.06 % B1, 12.98 % B2. 2.84 % G1 and 1.12% G2); and 14.65 for peanut (84.02 % B1, 8.82% B2, 5.01 % G, and 2.15% G?).
  • Küçük Resim Yok
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    Clinical and light microscopic studies of the conjunctival tissues of dogs with bilateral keratoconjunctivitis sicca before and after treatment with topical 2% cyclosporine
    (INFORMA HEALTHCARE, 2015) İzci, Celal; Çelik, İlhami; Alkan, Feyzanur; Erol, Muharrem; Sur, Emrah
    We determined the concentrations of goblet and immune cells in conjunctival imprints and tissues of canines with keratoconjunctivitis sicca (KCS) before and after cyclosporine A (CsA) treatment. Twelve dogs with bilateral KCS were assigned to three groups: untreated, treatment group 1, and treatment group 2. The treatment groups were treated topically with 2% ophthalmic CsA solution for 45 days; CsA treatment group 2 was followed for an additional 30 days after discontinuation of the drug. Schirmer tear test (STT) scores were recorded prior to CsA treatment and on alternate days throughout the experiment. CsA treatment improved the STT scores, restored conjunctival histology, increased goblet and epithelial cell numbers, and decreased numbers of inflammatory cells. Although the STT scores regressed slightly at day 30 after discontinuing the treatment, the scores were higher than the baseline values. Topical CsA treatment resolved clinical signs of KCS, improved STT scores and restored normal conjunctival histology.
  • Küçük Resim Yok
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    Değişik yoğunluktaki pulzatif ultrason tedavisinin kırık iyileşmesi üzerine etkisinin fiziksel, radyolojik ve mikroskobik olarak değerlendirilmesi
    (1997) İzci, Celal; Akvi, Sırrı; Alkan, Fahrettin; Çelik, İlhami; Sur, E.
    Çalışma, terapötik ultrasonun kırık iyileşmesi üzerine etkisini, fiziksel, radyolojik ve mikroskobik olarak belirlemek amacıyla yapıldı. Araştırma toplam 48 adet sağlıklı tavşan üzerinde dört grup halinde gerçekleştirildi. Tüm hayvanlara bilateral fibular osteotomi yapıldı. Operasyondan sonraki günden başlamak üzere, hayvanların sol fibulaları üzerine, gruplara göre sıra ile 02, 0.5, 1 ve 1.5 Watt/cm2 yoğunluğunda pulzatif ultrason uygulandı. Uygulama, 10 gün boyunca günde 10 dakikalık seanslar halinde yapıldı. Kontrol grubu olarak kullanılan sağ fibulalar üzerine ise aynı sürelerde ultrason cihazı çalıştırılmaksızın sadece prob friksiyonu uygulandı. Araştırmanın 0.7,15,21 ve30. günlerinde sağ ve sol fibulaların radyografisi alındı. Bir ay sonunda tavşanlara ötenazi yapılarak sağ ve sol fibulaları alındı. Her gruptaki ikişer çift fibula'nın histolojik muayenesi yapıldı. Diğer numunelerde kallus büyüklükler, medio-lateral ve aaterio-posterior eksenlerden, kompas ile ölçüldü. Aynı numunelerde basma ve çekme deneyleri yapılarak kallus mukavemeti değerlendirildi.
  • Küçük Resim
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    Determination of alpha-napthyl acetate esterase activity of native and frozen-thawed bull sperm acrosome
    (Selçuk Üniversitesi Veterinerlik Fakültesi, 2020) Canbolat, Ülkü Nur; Çelik, İlhami; Erdoğan, Rukiye
    Öz Amaç: Akrozomal enzimlerin seminal plazmaya sızıp sızmadığının belirlenmesi, suni tohumlama için sperma işlenmesi sırasında oluşan akrozom hasarının öncü bir göstergesi olabilir. Alfa-naftil asetat esteraz enzimi bu amaçla kullanılabilecek olan akrozomal enzimlerden biridir. Bu çalışmada, taze ve dondurup-çözdürülen boğa spermatozoonlarındaki alfa-naftil asetat esteraz enziminin pozitivite oranları ve lokalizasyonunun ışık mikroskobuyla belirlenmesi amaçlanmıştır. Gereç ve Yöntem: Çalışmada materyal olarak 7 boğadan alınan sperma örneği kullanıldı. Bu amaçla, taze ve dondurulup-çözdürülen sperma örneklerinden sürme frotiler hazırlandı. Bu frotilerde alfa-naftil asetat esteraz enzimi sitokimyasal metotla demonstre edildi. Hazırlanan preparatlar ışık mikroskobuyla incelenerek alfa-naftil-asetat esteraz pozitivite oranları, enzimin lokalizasyon ve dağılım tarzı belirlendi. Bulgular: Alfa-naftil asetat esteraz enzimi pozitivitesi, spermatozoonun akrozomunda lokalize olan kırmızımsı-kahve renkli reaksiyon ürünü halinde gözlendi. Bazı hücrelerde enzimatik reaksiyon akrozom zarında dar bir band halinde gözlendi. Taze sperma örneklerinin alfa-naftil asetat esteraz pozitivitesinde belirgin bireysel farklılıklar gözlendi. Enzimatik reaksiyon, hücrelerin tamamında aynı şiddette değildi. Bazı hücrelerdeki reaksiyon nispeten daha zayıftı. Düşük spermatozoon vitalitesi ve motilitesi tesbit edilen taze sperma örneklerinin alfa-naftil asetat esteraz enzim pozitivitesi de düşüktü. Dondurup-çözme işlemi, örneklerin alfa-naftil asetat esteraz pozitivitesinde önemli düşüşe yol açtı. Öneri: Akrozomal alfa-naftil asetat esteraz enzimi, boğa spermasının fertilite potansiyelinin ve dondurma işlemine uygunluğunun değerlendirilmesinde ve önemli bir parametre olarak dikkate alınabileceği sonucuna varılmıştır.
  • Küçük Resim Yok
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    Determination of the effects of aflatoxin B-1 given in ovo on the proximal tibial growth plate of broiler chickens: histological, histometric and immunohistochemical findings
    (TAYLOR & FRANCIS LTD, 2012) Öznurlu, Y.; Çelik, İlhami; Sur, Emrah; Özaydın, Tuğba; Oğuz, Halis; Altunbaş, Korhan
    Detrimental effects of aflatoxin B-1 (AFB(1)) on the embryonic development of broiler tibia and its proximal growth plate were determined by means of histological, histometric and immunohistochemical methods. For this, 420 fertile eggs from parent stocks of Ross 308 broiler chickens were divided into five groups according to the proposed treatment: a control untreated group, a group injected with 30% ethanol and three further groups to be injected with 5, 15 or 40 ng AFB(1). The eggs were injected into the air space prior to incubation. Five eggs from each group were opened at 9, 11, 13, 17, 19 and 21 days of incubation and tibial tissue samples were removed, dissected of muscle and connective tissues, and processed by means of routine histological techniques. The cell proliferation rate of the epiphyseal growth plate cells was determined by immunohistochemical assay of proliferating cell nuclear antigen (PCNA) expression. The results showed that both proliferative and hypertrophic zones narrowed significantly (P<0.05), when compared with the controls, in all of the AFB(1)-treated groups whereas the transitional zone thickened, especially in the group given 40 ng AFB(1) group. The PCNA positivity indices of 15 and 40 ng AFB(1)-treated groups were significantly higher (P<0.05) on days 11, 13, 17, 19 and 21 of incubation. It was concluded that in ovo-administered AFB(1) adversely affected embryonic development of the tibial growth plate, and that affected hatched broilers might also be more susceptible to skeletal disorders during growth.
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    Effect of Experimental Therapeutic Ultrasound on the Distal Antebrachial Growth Plates in One-Month-Old Rabbits
    (Bailliere Tindall, 2002) Oğurtan, Zeki; Çelik, İlhami; İzci, Celal; Boydak, Murat; Alkan, Fahrettin; Yılmaz, Koç
    The effect of therapeutic ultrasound (US) on the growth plates of growing bone was determined through fluorescence labelling and histopathological evaluation in one-month-old rabbits. Mean growth rates and mean heights of the growth plates of the US exposed radius vs. control radius, and US exposed ulna vs. control ulna showed no statistically significant differences at all intensities and time intervals. Mean growth rates and heights of the growth plates of the US exposed radius for 0.2 W/cm(2) (SATA) US vs. 0.5 W/cm(2) US showed the same type of pattern compared to those of the control radius for 0.2 W/cm(2) vs. 0.5 W/cm(2) at days 10, 15 and 20. Similar results were also found for the ulna. Effects of 0.5 W/cm(2) US on the mean growth rates and mean heights of the growth plates were superior to those of 0.2 W/cm(2) US at day 10. The results at day 10 were reversed for the same comparisons at days 15 and 20. No cavitation effect of US was observed on growth plate cells. There was no evidence of partial or complete premature closure of the growth plates or of angular deformity of the carpal region.
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    Effect of Melatonin Supplementation on Plasma Glucose and Liver Glycogen Levels in Rats Subjected to Acute Swimming Exercise
    (UNIV KARACHI, 2010) Kaya, Oktay; Kılıç, Mehmet; Çelik, İlhami; Baltacı, Abdulkerim Kasım; Moğulkoç, Rasim
    The aim of the present study is to examine how melatonin supplementation affects plasma glucose and liver glycogen levels in rats subjected to acute swimming exercise. Spraque-Dawley species thirty adult male rats were allocated to 3 groups with equal number of animals: general control group which was not subjected to any procedure (Group 1), the group subjected to a 30-minute acute swimming exercise (Group 2), and the group subjected to a 30-minute acute swimming exercise after intraperitoneal (i.p.) melatonin supplementation (3 mg/kg/day) for 4 weeks (Group 3). Blood samples collected from the experimental animals by decapitation method were analyzed in terms of plasma glucose, and glycogen levels were determined in liver tissue samples by histological method. The highest plasma glucose levels were obtained in group 2 (p<0,05). Plasma glucose levels in groups 1 and 3 were not different. Mean liver glycogen level in group 3 was significantly higher than those in the other groups (p<0,01), while there was no significant difference between group 1 and group 2 in terms of this parameter. Results of the study demonstrate that melatonin supplementation can have a protective effect on liver glycogen reserves in rats subjected to acute swimming exercise.
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    Effects of Aflatoxin B-1 on the Development of the Bursa of Fabricius and Blood Lymphocyte Acid Phosphatase of the Chicken
    (Taylor & Francis Ltd, 2003) Sur, Emrah; Çelik, İlhami
    1. In this study, embryotoxicity and effects of in ovo administration of aflatoxin B-1 (AFB(1)) on the embryonic development of the bursa of Fabricius were determined in fertilised chicken eggs by histological methods and histochemical demonstration of acid phosphatase (ACP-ase) enzyme. Peripheral blood lymphocyte percentages were also estimated. 2. Embryonic stages were determined according to the Hamburger-Hamilton (H-H) scale. Mortality rates increased in AFB(1)-injected groups in a dose-dependent manner. Embryonic deaths were concentrated at H-H 31 in the 5 ng AFB(1)/egg group while the deaths were highest at H - H 25 in the 10 and 20 ng AFB(1)/ egg groups. In the 40 ng AFB(1)/ egg group, embryonic deaths mostly occurred during the first 70 to 72 h of incubation ( H - H 20). 3. Bursal development was quite similar at d 7 of incubation in control and all experimental groups although development had been substantially impaired and retarded at d 10 of incubation in 10, 20 and 40 ng AFB(1)/ egg groups. A gradual decrease of ACP-ase positive lymphocyte numbers was observed with increasing AFB(1) doses. The chicks in AFB(1)-treated groups hatched with poorly developed bursae compared with those of the controls. However, proportions of ACP-ase positive lymphocytes substantially increased at hatch and at 21 d of incubation all groups had similar numbers. 4. The results revealed that breeder diets should be investigated for aflatoxins and specifically AFB(1), in cases of low hatchability and flock immunity, because low concentration of AFB(1) transferred into the fertilised eggs might be the cause of serious problems.
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    Effects of Long-term 50 Hz Magnetic Field Exposure on the Micro Nucleated Polychromatic Erythrocyte and Blood Lymphocyte Frequency and Argyrophilic Nucleolar Organizer Regions in Lymphocytes of Mice
    (Maghira & Maas Publications, 2010) Okudan, Nilsel; Çelik, İlhami; Salbacak, Ahmet; Çiçekçibaşı, Aynur Emine; Büyükmumcu, Mustafa; Gökbel, Hakkı
    OBJECTIVES: We aimed to investigate the effects of weak extremely low frequency electromagnetic fields (ELF-EMFs) on the nucleus size, the silver staining nucleolar organizer regions (AgNORs), the frequency of micro nucleated peripheral blood lymphocytes (MPBLs) and the micro nucleated polychromatic erythrocytes (MPCEs). METHODS: One hundred and twenty Swiss albino mice were equally divided into 6 groups. The study groups were exposed to 1, 2, 3, 4 and 5 mu T 50 Hz-EMFs for 40 days. Micronucleus number (MN) per PBL was determined.. RESULTS: ELF-EMF exposure caused a nonlinear decline of nucleus area. A sharp drop occurred in AgNOR area of 1 mu T group, and following it gained an insignificantly higher level than that of the control group. The field did not change mean AgNOR numbers per nucleus of the groups. Relative AgNOR area had the highest level in 1 mu T-exposure group, and the level was quite similar to that of the 5 mu T-exposure group. The remaining groups had significantly lower values quite similar to that of the control level. The field exposure at any intensity did not affect significantly the frequency of either MPBLs or MPCEs. The number of MN per PBL in the 4 and 5 mu T-exposure groups were significantly higher than those of the lower intensity exposure groups. The males in 4 mu T-exposure group displayed the highest MN number per PBL, whereas values changed in a nonlinear manner. CONCLUSIONS: The results of the present study suggest that <= 5 mu T intensities of 50 Hz EMFs did not cause genotoxic effect on the mouse.
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    Effects of Zinc Deficiency and Supplementation on the Glycogen Contents of Liver and Plasma Lactate and Leptin Levels of Rats Performing Acute Exercise
    (Humana Press Inc, 2003) Baltacı, Abdülkerim Kasım; Özyürek, Kürşat; Moğulkoç, Rasim; Kurtoğlu, Erdal; Özkan, Yasemin; Çelik, İlhami
    The aim of the present study was to investigate how zinc (Zn) deficiency and supplementation affect glycogen content of the liver and plasma lactate and leptin levels of rats performing acute swimming exercise just before the blood samples were obtained. Four sets of 10 rats each served as the (1) Zn-deficient group, (2) Zn-supplemented group, (3) swimming controls, and (4) normal controls. Plasma lactate levels of Zn-deficient animals were significantly higher than those in the other three groups (p < 0.01), and those in the swimming controls (group 3) were significantly higher than in the Zn-supplemented animals, group 2 (p < 0.01). The plasma glucose of the Zn-deficient group was significantly higher than all other groups (p < 0.01) and that of group 2 was significantly lower than group 4 (p < 0.01). Glycogen levels in liver of the Zn-deficient animals was significantly lower than groups 2 and 4 (p < 0.01), and, in turn, were higher than for group 3 (p < 0.01). The plasma leptin and Zn levels of group 1 were significantly lower than in all other groups (p < 0.01). These results suggest that Zn deficiency exerts a negative influence in the above-mentioned parameters and that Zn supplementation has the opposite effect.
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    Effects of Zinc Supplementation to Ration on Some Hematological Parameters in Broiler Chicks
    (Humana Press Inc, 2002) Dönmez, Nurcan; Dönmez, Hasan Hüseyin; Keskin, Ercan; Çelik, İlhami
    The aim of the study was to examine the effects of zinc supplementation on some hematological parameters. Sixty newborn male broiler chicks were utilized in the study. Zinc (Zn) was added into drinking water at levels of 0, 125, 500, and 1000 mg/kg. In the study, there was no significant difference between control and Zn-supplemented groups in erythrocyte count, hemoglobin amount, hematocrit levels, total leukocyte count, and differential leukocyte % levels, but the alpha-naphthyl acetate esterase ANAE(+) lymphocyte rate significantly (p< 0.05) increased in the 125-ppm Zn-supplemented group compared with the control group. In conclusion, the data obtained may be beneficial in demonstrating the effects of zinc on, at least, these parameters.
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    Enzyme Histochemistry and Agnor Numbers in the Peripheral Blood Leukocytes of 6 Month-old Kangal Bred Anatolian Shepherd Dogs
    (Ecole Nationale Veterinaire Toulouse, 2003) Sur, Emrah; Çelik, İlhami; Öznurlu, Y.; Aydın, Mehmet Faruk; Şen, İsmail; Özparlak, H.
    The determination of peripheral blood lymphocyte (PBL) counts and the, enzyme profile of PBL as reference values would be the first step to identify markers of lymphocyte differentiation and / or lymphoproliferation. In healthy, 6 month-old Kangal Shepherd dogs, PBL percentages, enzyme activities (cc-naphtyl acetate esterase (ANAE), acid phosphatase (ACP-ase), P-glucuronidase (BG-ase) and N acetyl beta-glucosaminidase (NABG-ase)) and some characteristics of silver stained nucleolus organizing regions (AgNOR parameters) were evaluated by histochemical methods. PBL percentage in females was significantly lower when compared to males (p < 0.05). Mean ANAE-positivity of PBL in males was 63.13 % whereas the positivity was slightly lower in females (60.75 %). The percentages of ACP-ase, BG-ase and NABG-ase positive lymphocytes of both genders were close similar. Values were respectively 39.37 %, 55.11 % and 52.45 % in males, and 39.12 %, 52.73 % and 51.37 % in females. There was no significant difference (p > 0.05) in mean AgNOR number nor in AgNOR diameters in PBL between males and females. Nucleus diameters and AgNOR diameter / nucleus diameter ratios of both genders were also quite similar. It was concluded that tabulated data as reference sources should be established for enzymatic positivity profiles and for AgNOR parameters in different age groups of Kangal breed and in other popular dog breeds. These data might be useful in early diagnosis of lymphoproliferative disturbances and in viral diseases caused by lymphotroph viruses and in the planning of therapeutic protocols, by using relatively cheaper techniques.
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    Enzyme Histochemistry of the Peripheral Blood Lymphocytes in Arabian Horses
    (Medwell Online, 2010) Aydın, Mehmet Faruk; Çelik, İlhami; Sur, Emrah; Öznurlu, Yasemin; Telatar, Tuğba
    The aims of this study were to determine the percentages of A-Naphthyl Acetate Esterase (ANAE) positive and Acid phosphatase (Acp)-postive Peripheral Blood Lymphocytes (PBL) and the proportion of peripheral blood lymphocytes in the Arabian race horses. Blood samples obtained from the Vena jugularis of 40 healthy Arabian race horses at four years old were analyzed. The animals were selected randomly from found as 46.20%. In conclusion, the enzyme histochemical techniques are simple, inexpensive and well performed for laboratory conditions. Also, these techniques may help early diagnosis of different diseases. Further studies about enzyme histochemistry in Arabian race horses shouled be performed.
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    Erken Embriyonal Dönemde Yumurtaya Verilen Testosteron Propiyonat'ın Tavuk Bursa Fabricii'si Üzerindeki Etkileri
    (Selçuk Üniversitesi, 1998) Çelik, İlhami; Dönmez, Hasan Hüseyin
    The aim of this study was to investigate the effects ol in ovo administrated testosterone propionate (TP) on the embryonıc development ol bursa ol Fabricius and also to determine the histological changes occuring at posthathcing period. In the study, toıally 350 lertilized eggs ol a hybrid breed, B55 were usecl. The eggs were divided into two groups. First-group eggs were served as controls, in the second group-eggs, which were treated with tesıosterone propionate, constituted experimentals. light microscopic observations have showed that in ovo hormonal bursectomy adversely allected Iymphoid follicle development via blocking the epithelial bud formation. Nevertheless, TP-treatment did noı compleıely prevented folliele formation in the organ; atrophic and degenerated lollicles wilh cysts were also seen, a/lhough they were in smail numbers. In TP-trealed group, the organ was smailer than those of conlrols and most ol the Iymphoid follicles have nol compleled their development. At the lirst week of post·halching period Iymphoid inliltration areas and cysts were obseNed in the lamina propria ol the organ. At the 8th week ol post-hatching period, a rudiment of the organ which was mainly constituted of the connective tissue conıaining diffuse Iymphoid infillration areas was observed.