Yazar "Acar, H." seçeneğine göre listele
Listeleniyor 1 - 20 / 34
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Are There Interchromosomal Effects of Chromosomal Rearrangements on Occurrence of Aneuploidy in Sperm Nuclei of Carriers?(Nature Publishing Group, 2002) Acar, H.; Yakut, T.; Çora, Tülün; Egeli, Ünal; Kaynak, M.; Yıldırım, S.[Abstract not Available]Öğe A Case of Ambiguous Genitalia Presenting with a 45,x/46,xr(Y)(P11.2;q11.23)/47,x,idic(Y)(P11.2),idic(Y)(P11.2) Karyotype(EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER, 2001) Dündar, M.; Lowther, G.; Acar, H.; Kurtoğlu, S.; Demiryılmaz, F.; Küçükaydın, M.An infant with ambiguous genitalia was found to have a karyotype 45,X/46,X,r(Y)(p11.2;q11.23)/47,X, idic(Y)(p11.2),idic(Y)(p11.2) using G-banding, C-banding and FISH. Examination of the genitalia revealed a phallus measuring 1.5 cm in length and 0.5 cm wide with perineal orifice. Subtle phenotypic features consistent with Turner syndrome were not present. Genital ultrasonography revealed the presence of an infantile uterus. Endoscopy of the vagina, uterus and cervix appeared normal.Öğe A case with mental retardation and a translocation between chromosomes 6 and Y(KARGER, 1997) Durakbaşı, Hatice Gül Dursun; Zamani, A.; Acar, H.; Acar, A.[Abstract not Available]Öğe CLINICAL IMPORTANCE OF B2 GLYCOPROTEIN I ANTIBODIES IN BCR-ABL NEGATIVE MYELOPROLIFERATIVE NEOPLASMS(FERRATA STORTI FOUNDATION, 2016) Kocak, M. Z.; Dagli, M.; Kutlucan, A.; Basturk, A.; Unlu, A.; Acar, H.; Kutlucan, L.[Abstract not Available]Öğe Coexistence of pericentric inversion of chromosome Y and chromosome 15p+ in a case detected with cytogenetic and FISH techniques(KARGER, 1997) Acar, H.; Çora, Tülin; Erkul, I.; Acar, Aynur[Abstract not Available]Öğe Comparison of semen profile and frequency of chromosome aneuploidies in sperm nuclei of patients with varicocele before and after varicocelectomy(WILEY, 2009) Acar, H.; Kilinc, M.; Guven, S.; Yurdakul, T.; Celik, R.Semen profile and meiotic segregation products are important for assessing aneuploidy risk and risk of resulting infertility. To determine the effect of varicocelectomy on semen profile and aneuploidy frequency, we investigated semen profile and aneuploidy frequency of selected chromosomes in patients with varicocele before and after varicocelectomy. Chromosomal aneuploidy for selected chromosomes was evaluated using chromosome-specific DNA fluorescence in situ hybridisation (FISH) probes. There was a significant difference in the level of normal sperm morphology before and after varicocelectomy (P > 0.007). There were no significant differences in aneuploidy frequency of chromosomes 1, 16, 17 and 18 in sperm nuclei obtained from patients before varicocelectomy compared with 6-7 months after varicocelectomy (P > 0.05), although FISH analysis with chromosomes 17 and 18 combination showed a higher aneuploidy frequency before varicocelectomy than after varicocelectomy (7.81 +/- 9.67 versus 4.03 +/- 1.46 respectively). In conclusion, varicocele seems to affect the semen profile but minimally affects aneuploidy frequency. Varicocelectomy demonstrates a repairing effect on the semen profile and contributes to a slight decrease in aneuploidy frequency in some but not all chromosomes.Öğe Cytotoxicity evaluation of dentin contacting materials with dentin barrier test device using erbium-doped yttrium, aluminum, and garnet laser-treated dentin(SAGE PUBLICATIONS LTD, 2014) Ulker, H. E.; Ulker, M.; Botsali, M. S.; Dundar, A.; Acar, H.Objectives: The effect of dentin contacting materials on three-dimensional cultures of pulp-derived cells was evaluated in a dentin barrier test device using erbium-doped yttrium, aluminum, and garnet (Er:YAG) laser-treated dentin. Methods: The test materials (iBond (R), G-Bond (TM), and Vitrebond (TM)) were applied on laser-treated or untreated dentin discs. After 24 h of exposure with perfusion of the test chamber, cell survival was evaluated by enzyme activity and related to a nontoxic control material. The mean values of control tissues were set to represent 100% viability. Data were analyzed using Kruskal-Wallis and Mann-Whitney U test. Results: Vitrebond was the most toxic material for both laser-treated and untreated dentin. On untreated dentin, G-bond was cytotoxic to the pulp-derived cells (p < 0.05), and iBond was similar to the negative control group (p > 0.05). However, G-Bond and iBond were not cytotoxic when they were applied to Er: YAG laser-treated dentin (p > 0.05). Conclusion: Er: YAG laser treatment of dentin may protect the pulp cells from toxic substances of dentin contacting restorative materials; however, this effect is material related. Taking into consideration the limitations of this in vitro study, the Er: YAG laser treatment of dentin before restoration might be an option for decreasing the cytotoxic effects of the dental materials. Further research is required for clinical applications.Öğe Detection of an Unbalanced T(4;15) by Fish in a Child With Multiple Congenital Anomalies(Medecine Et Hygiene, 2001) Celep, Figen; Acar, H.; Aynacı, O.; Aynacı, F. M.; Karagüzel, A.Detection of an unbalanced t(4; 15) by FISH in a child with multiple congenital anomalies: In this report, we present the clinical history and findings in a 6-month-old male with multiple congenital anomalies, developmental delay, and an initial male karyotype with 4q+. The origin of the additional segment on 4q was unequivocally established by fluorescence in situ hybridization (FISH). Whole chromosome probe for chromosome 4 and chromosome 15-specific a-satellite probe were used. The karyotype was demonstrated to be 46,XY,der(4), t(4;15) (q35;?), inv(9)(p13q13). To the best of our knowledge the above cytogenetic abnormalities with these clinical findings have not been described previously. This case further demonstrates the advantage of FISH in the identification of anomalous chromosome regions and breakpoints.Öğe Detection of monosomy 22 in interphase nuclei by fluorescence in situ hybridization(KARGER, 1997) Acar, H.; Acar, Osman; Acar, A.; Üstün, Mehmet Erkan[Abstract not Available]Öğe Determination of distribution of chromosomes in the spreads(SPRINGER, 2009) Kocak, N.; Ozen, F.; Acar, H.[Abstract not Available]Öğe Different Chromosome Y Abnormalities in Turner Syndrome(MEDECINE ET HYGIENE, 2001) Bağcı, G.; Acar, H.; Tomruk, H.Different Chromosome Y Abnormalities in Turner Syndrome: A 17-year-old phenotypically female girl was referred for evaluation because of short stature and primary amenorrhea. Cytogenetic analysis showed a mosaic 46,XY/45,X/47,XYY/46,X,idic(Yq)/47,XY,idic(Yq)/48,XXY,idic(Yq)/46,X,t(C:Y) karyotype. Conventional cytogenetic results were supplemented with fluorescence in situ hybridization (FISH) techniques to ensure a better characterization of abnormalities. By using FISH, a supernumerary marker chromosome derived from chromosome Y which could not be detected by conventional cytogenetics was revealed. Furthermore, additional abnormalities and their frequencies were highlighted by the application of DNA probes specific for X and Y chromosomes. Thus, FISH proved useful in determining low frequency cell lines which would need analysis of a large number of good quality metaphase spreads by conventional cytogenetic techniques; it helped in Identifying the nature and the origin of unknown markers and rearrangements which have important implication in sexual differentiation and development of gonadal tumours.Öğe Dosimetric comparison of two different whole brain radiotherapy techniques in patients with brain metastases: How to decrease lens dose?(IJRR-IRANIAN JOURNAL RADIATION RES, 2014) Yavas, G.; Yavas, C.; Gul, O. V.; Acar, H.; Ata, O.Background: Palliative whole brain radiotherapy (WBRT) has been the standard treatment for brain metastases. Ionizing radiation is known to be one of the most potent cataractogenic agents. We aimed to evaluate two different radiotherapy techniques with respect to the doses received by the organs at risk (OAR) in patients with brain metastasis who undergone WBRT. Materials and Methods: Ten consecutive patients with brain metastasis were included. For each patient, two different treatment plans were created for whole brain. Helmet-field (HF) (anterior border was 2 cm posterior to lens, inferior border was the bottom of C2 vertebra) and classical technique with collimation (CT) (anterior border was defined as skin fall off, inferior border was the bottom of cranial base) were generated for all patients. Two techniques were compared with respect to the doses received by the OAR including bilateral lenses, optic nerves and eye-balls, the dose homogeneity index (DHI), and the monitor unit counts (MU) required for the treatment. Student-t test was used for statistical analysis. Results: There was no difference between two techniques in terms of both DHI (p: 0.182) and MU counts (p: 0.167). The maximum and mean doses received by the right lens, left lens and right eye-ball were significantly reduced with CT (p values for maximum doses 0.007, 0.012 and 0.010; for median doses 0.027, 0.046 and 0.002 respectively). Conclusion: CT was found to be more advantageous, with respect to the lens doses in addition the dose received by the right eye-ball during WBRT.Öğe Dosimetric comparison of whole breast radiotherapy using field in field and conformal radiotherapy techniques in early stage breast cancer(IJRR-IRANIAN JOURNAL RADIATION RES, 2012) Yavas, G.; Yavas, C.; Acar, H.Background: We aimed to compare field-in-field technique (FIF) with conformal tangential field radiotherapy (CRT) in terms of dosimetric benefits for early stage breast cancer radiotherapy. Materials and Methods: Twenty consecutive left-side breast cancer patients who underwent breast-conserving surgery were included to the study. For each patient, two different treatment plans were created for the entire breast. FIF plans and CRT plans were compared for doses in the planning target volume (PTV), the organ at risk (OAR) volume including ipsilateral lung, heart, left ascending coronary artery (LAD) and the contralateral breast, the homogeneity index (HI), and the monitor unit (MU) counts required for the treatment. Paired samples t-test was used for statistical analysis. Results: The FIF technique significantly reduced the maximum dose of the PTV as well as the mean doses of the heart, LAD, ipsilateral lung and the contralateral breast (p values were <0.001 for each). When the OAR volumes irradiated with 2, 5, 10, 20, 30 and 40 Gy were compared, the results were in favor of the FT technique. The volume receiving <20 Gy of the prescription dose for the ipsilateral lung was significantly decreased using FIF technique (p<0.001). FIF technique allowed us more homogenous dose distribution with lower MUs. Conclusion: The FIF technique provided better dose distribution in the PTV and significantly reduced the doses in the OAR. Considering the lower MUs required for treatment the FIF technique seems to be more advantageous than CRT during whole breast irradiation. Iran. J. Radiat. Res., 2012; 10(3-4): 131-138Öğe Effect of the FSH receptor polymorphism on the age at menarche(7847050 CANADA INC, 2019) Kerimoglu, O. S.; Pekin, A.; Yilmaz, S. A.; Incesu, F. N.; Nergiz, S.; Dogan, N. U.; Acar, H.Estrogen and estrogen receptor (ER) genes have an effect on the age at menarche. Follicle stimulating hormone (BID is an important hormone that plays a major role in the regulation of folliculogenesis and estrogen synthesis. The aim of this study was to investigate the influence of the FSH receptor (FSHR) polymorphisms Asn680Ser and Thr307Ala on age at menarche. Materials and Methods: FSH receptor gene polymorphisms were investigated in women who were admitted to Selcuk University between May 2013 and November 2014. Information about age at menarche was obtained through interviews. Polymorphic analysis of the FSH receptor gene was performed in 209 healthy female patients who had undergone an annual health examination. Genomic DNA was obtained from peripheral blood leukocytes, and polymorphisms were investigated using restriction fragment length polymorphism analysis. Results: The mean age at menarche was 12.95 +/- 1.62 years. The overall frequencies for Asn680Ser variants NN, NS, and SS were 40.2%, 36.4%, and 23.4%, respectively, whereas Thr307Ala variants TT, TA, and AA were 34.4%, 41.1%, and 24.4%, respectively. Menarche occurred 3.6 months earlier in subjects with TN homozygote genotype than in AS homozygotes, and the mean age at menarche was 2.5 months earlier in TN homozygotes compared with that in all other study subjects, but the differences were not statistically significant (p = 0.966 and p = 0.986, respectively). Conclusions: The Ala307Thr and Ser680Asn polymorphisms of the FSHR gene are not associated with age at menarche.Öğe Effects of kras gene lcs6 mutation on metastasis pathways in human lung cancer(NATURE PUBLISHING GROUP, 2019) Azzawri, A. A.; Cora, T.; Acar, H.[Abstract not Available]Öğe Evaluation of doses in the Wernicke and Broca's areas using two different technique in patients with right frontal glioblastoma multiforme(IJRR-IRANIAN JOURNAL RADIATION RES, 2013) Acar, H.; Yavas, G.; Yavas, C.Background: Broca's and Wernicke's areas, which are important language areas of brain, are commonly irradiated in patients with right frontal lobe glioblastomamultiforme (GBM). We aimed to compare two different treatment planning techniques in terms of the doses in the Broca's and Wernicke's areas for the patients with right frontal GBM. Materials and Methods: Two different treatment planning techniques of right frontal GBM were generated for Rando phantom: two field technique using parallel opposed beams and three field techniques. Both plans were compared for doses in planning target volume (PTV), Broca's and Wernicke's areas. Additionally to test the accuracy of treatment planning system (TPS) dose calculation; thermoluminescent dosimeters (TLD) were used. Results: The three field technique allowed the lower doses in the Broca's and Wernickes's areas. The doses calculated in the Broca'sand Wernicke's areas were 0.98% +/- 0.03 and 0.09% +/- 0.06 of the isocenter dose with three field and 1.06% +/- 0.04 and 0.133% +/- 0.03 of the isocenter dose with two field techniques respectively. When the doses measured by TLD and calculated with TPS were compared; the differences were 3.23% and 2.92 % for Broca's area, and 4.12 % and 3.95% for Wernicke's area for two field and three field techniques respectively. Conclusion: There was a good agreement between TPS and TLD calculations for both techniques. Three field techniques seem to be more advantageous than two field technique with respect to doses of Broca's and Wernicke's areas. However this finding should be clarified with further clinical studies.Öğe Evaluation of the Association Between Null Genotypes of Glutathione-S-transferases and Behcet's Disease(Springer, 2006) Uzunoğlu, S.; Acar, H.; Okudan, Nilsel; Gökbel, Hakkı; Mevlitoğlu, I.; Sarı, F.Glutathione S-transferases (GST) play an important role in oxidative stress related syndromes. An imbalance of the oxidant and antioxidant systems is important in the pathogenesis of Behcet's disease (BD). The objective of this study was to evaluate the association of null genotypes of GST-M1 and GST-T1 with BD since some preliminary molecular genetic data were recently published. Ninety-four Turkish BD patients (42 male, 52 female, 37.1 +/- 10.4 years) and 140 healthy volunteers (70 male, 70 female, 36.8 +/- 11.7 years) matched for age and gender with the patients as the control group were included in the study. Distributions of GST-M1 and GST-T1 genotypes were determined by multiplexed PCR using three sets of primers for GST-M1, GST-T1, and beta-globulin genes. There was no association between BD and the frequencies of GST-M1 and GST-T1 null genotypes when compared to controls by separate analysis. However, by cross and pooled combination analysis there was a significant association between the frequencies of pooled GSTs with one or both null genotypes in BD and controls. This is the first evidence that the association between the frequencies of GST-M1 and GST-T1 null genotypes and BD might be dependent on the interaction of multiple null allele polymorphisms rather than a single null allele of GST-M1 and GST-T1.Öğe A girl with mos45,X/46,XY/47,XYY: Genetic and clinical findings(SPRINGER, 2009) Cora, T.; Acar, H.; Atabek, E.; Balasar, Oe.; Nergis, S.[Abstract not Available]Öğe Glutathione S-Transferase M1 and T1 Polymorphisms in Turkish Patients With Varicocele(WILEY-BLACKWELL, 2012) Acar, H.; Kılınç, M.; Güven, S.; İnan, Z.Several studies have shown that high oxidative stress levels are associated with varicocele. The GST (glutathione S-transferase) family of genes is critical in the protection of cells from oxidative stress because they utilise as substrates a wide variety of products of oxidative stress. The objective of this study was to assess the relationship between genetic polymorphism in GST-M1 and GST-T1 and varicocele using 109 varicocele patients and 123 controls. Varicoceles were clinically graded as Grade 1, Grade 2 and Grade 3. GST-M1 and GST-T1 genes were determined by polymerase chain reaction. Although the GST-M1 null genotype was higher in Grade 3 than in Grade 1, 2 and controls, there were no statistical differences between control group and varicocele groups according to GST-M1 and GST-T1 null genotype. Men with varicocele do not have more GST-M1 and GST-T1 null polymorphisms than men without varicocele. Additional studies are needed to assess the exact mechanism by which the varicocele corresponds to elevated ROS levels.Öğe Identification of Classic and Complex T(15;17) and/or Rar Alpha/Pml Gene Fusion in Apl by Cytogenetic and Dual Color-Fish Techniques(1997) Acar, H.; Dündar, M.; Stewart, J.Acute promyelocytic leukemia (APL) is a malignant condition characterized by t(15;17)(q22;q12), which fuses the PML gene on chromosome 15 to the retinoic acid receptor alpha (RAR alpha) gene on chromosome 17. In this study, t(15;17) was identified cytogenetically by using the conventional cytogenetic technique, and its molecular counterpart RAR alpha/PML fusion on chromosome 17 on interphase nuclei was further confirmed by means of dual color- (DC-) fluorescence in situ hybridization (FISH) on serial bone marrow (BM) and peripheral blood (PB) samples from APL patients at different stages of the disease. Overall, our findings indicate that interphase DC-FISH analysis can be a useful technique as an adjunct to conventional cytogenetic investigation for detecting the presence of RAR alpha/PML fusion in APL.
