Yazar "Celik, Ilhami" seçeneğine göre listele
Listeleniyor 1 - 19 / 19
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Apoptosis: an underlying factor for accelerated periodontal disease associated with diabetes in rats(SPRINGER HEIDELBERG, 2014) Tunali, Mustafa; Ataoglu, Tamer; Celik, IlhamiDiabetes mellitus (DM) is well-established risk factor for periodontal disease. DM can also lead to changes in the number of apoptotic cells in periodontal tissues. The goal of this study was to evaluate apoptosis, depending on DM, in healthy and diseased periodontal soft tissues. A total of 43 adult male Sprague-Dawley rats were used in this study. Experimental periodontitis was created by placing silk ligatures around the cervices of the first mandibular molars. Experimental diabetes was induced by intraperitoneal injection of the diabetogenic agent streptozotocin (STZ). Following the induction of both experimental diseases, the animals were divided into four groups: (1) The healthy group (H) (n = 10); (2) The diabetes group (D) (n = 10); (3) The periodontitis group (P) (n = 11); and (4) The diabetes and periodontitis group (DP) (n = 12). Apoptotic cells were determined by immunohistochemistry, and the frequency of apoptotic cells was evaluated by apoptotic index score. It was observed that there was less apoptosis in both the epithelial and gingival connective tissue cells of healthy diabetic tissues than in healthy tissues without diabetes. When periodontal disease existed, apoptosis increased in both the epithelial and gingival connective tissues of diabetic and non-diabetic animals. There may be differences in the apoptotic mechanisms in the periodontal soft tissues of diabetic and non-diabetic animals. Apoptosis may be one of the underlying factors in increased risk for periodontal disease that is associated with diabetes.Öğe Comparative Skin Histology of the White New Zealand and Angora Rabbits: Histometrical and Immunohistochemical Evaluations(MEDWELL ONLINE, 2009) Oznurlu, Yasemin; Celik, Ilhami; Sur, Emrah; Telatar, Tugba; Ozparlak, HalukIn this study, thickness of the skin sub layers and the number, absolute and relative areas of the nuclear Argyrophilic Nucleolar Organiser Regions (AgNORs) and the number of Proliferating Cell Nuclear Antigen (PCNA) positive nuclei of the germinal matrix epithelial cells of the Angora and white New Zealand rabbits were compared. Skin samples were taken from 40 adult healthy rabbits. General histological features of the skin in both Angora and white New Zealand rabbits were quite similar and displayed the characteristics of thin skin. Angora rabbit skin was significantly (p<0.05) thicker than that of the white New Zealand rabbit and the difference mainly originated from the thicker papillary layer of the dermis. The Angora rabbit had the greatest hair follicle number in the unit area of the dermis. The shape, dimension and distribution pattern of the AgNORs were similar in both rabbit strains. The number, absolute and relative areas of the AgNORs and PCNA immunoreactivity of the Angora rabbits were higher than those of the White New Zealand rabbits. The histological characteristics of the skin in the white New Zealand rabbit were suitable for leather industry, whereas the Angora rabbit had good hair follicle characteristics for wool production. Further studies on a larger population regarding seasonal differences should be done to elucidate the possible association between AgNOR parameters and PCNA immunoreactivity of germinal matrix cells of hair follicles and wool production.Öğe Determination of the activity of alpha naphthyl acetate esterase and acid phosphatase of peripheral blood lymphocytes in healty pregnant merino sheep(KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2013) Sur, Emrah; Aydin, Ibrahim; Oznurlu, Yasemin; Ozaydin, Tugba; Celik, Ilhami; Kadiralieva, NaristeThis study was performed to determine the effects of pregnancy on the activities of alpha naphthyl acetate esterase (ANAE) and acid phosphatase (ACP-ase) of the peripheral blood lymphocytes in pregnant Merino sheep. Peripheral blood lymphocyte percentages were also estimated. Periferal blood samples were taken from animals in six different gestational stages as non-pregnant control, in the first, the second, the third, the fourth and the fifth month of pregnancy. Each group was contained 20 animals. The lowest ANAE (+) lymphocytes percentage (63.5%) was determined in the first month of pregnancy whereas the highest null lymphocytes proportion (12.75%) was detected in the same gestational period. There were statistically decreases in the proportions of peripheral blood lymphocyte (42.9%) and the ACP-ase (+) lymphocytes (43.35%) in the last gestational stages. Although the posibble hormonal changes may cause the fluctuation of peripheral blood lymphocyte proportions in all gestational periods, the most distinctive changes were observed at the begining and at the end of the pregnancy. It was concluded that the data was obtained from this study was useful for understanding of the possible mechanisms of maternal tolerance.Öğe Determination of the effects of extremely low frequency electromagnetic fields on the percentages of peripheral blood leukocytes and histology of lymphoid organs of the mouse(SAUDI MED J, 2008) Cicekcibasi, Aynur E.; Celik, Ilhami; Salbacak, Ahmet; Ozkan, Yasemin; Okudan, Nilsel; Buyukmumcu, MustafaObjectives: To determine the effects of very weak, extremely low frequency (50 Hz) electromagnetic field (ELF-EMF) on the relative spleen weight, lymphoid organ histology, peripheral blood leukocyte and alpha-naphthyl acetate esterase positive (ANAE-positive) lymphocyte percentages of the mouse. Methods: The study was carried out in Scientific Research and Application Center of Selcuk University, Konya, Turkey in 2005. A total of 120 Swiss albino mice were divided into 6 groups (20 in each group). The experimental animals were exposed to 1, 2, 3, 4 and 5 mu T flux intensities (rms) of EMF at 50 Hz for 40 days. Results: In the exposure groups with 20 animals, the body weight (BW) increased gradually in higher field intensities and reached at peak level in the 4 mu T, and then slightly decreased. The relative spleen weight (% of the BW) was not affected. The ELF-EMF treatment did not cause any significant change in lymphocyte, monocyte and ANAE-positive lymphocyte ratios, whereas percentages of neutrophils and basophiles changed non-linearly. Any change in the lymphoid organ histology, which is attributable to the field effect, was not observed in the exposure groups. Conclusion: Extremely low frequency-EMF exposure with the flux intensities between 1-5 mu T for 40 days did not cause any effect on the relative spleen weight, lymphoid organ histology, leukocyte and ANAE-positive lymphocyte percentages of the mouse.Öğe The distribution of pas-positive uterine natural killer (unk) cells in the decidua basalis of pregnant mice(KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2015) Sur, Emrah; Oznurlu, Yasemin; Ozaydin, Tugba; Celik, Ilhami; Aydin, Ibrahim; Kadyralieva, NaristeThis study was carried out to determine the distribution of uterine natural killer (uNK) cells having periodic-acid Schiff (PAS) positive granules in the decidua basalis in pregnant mice. For this purpose, mice at 12-14 weeks of age were divided into four groups as nonpregnant control, and at the middle of the first, second, and the third week of the pregnancy, corresponding to early, middle, and late (3rd, 10th, and 17th days of pregnancy) gestational stages respectively (n=6 for each group). After placentation, it was identified four different regions as metrial region (MLAp; mesometrial lymphoid aggregate of pregnancy), decidua basalis, junctional zone and labyrinth zone in mice. It was observed trophoblast giant cells between decidua basalis and junctional zone. The number of PAS-positive uNK cells was found as 5 in 10.000 mu m(2) in endometrium of control animals while it was determined as 26.5 number/10.000 mu m(2) in decidua basalis at early gestational period. The highest PAS-positive uNK cell numbers (56 number/10.000 mu m(2)) were detected at the mid-gestational period in decidua basalis. uNK cells were also observed in the lumen of the maternal blood vessels. In mesometrial region, the dilatation of maternal blood vessels and thinning of the media layer was distinct. It was concluded that the number and the distribution of the uNK cells having periodic-acid Schiff (PAS) positive granules was affected by pregnancy.Öğe Effects of aflatoxin on the proportions of peripheral blood leukocytes and alpha-naphtyl acetate esterase (anae) positive lymphocytes in the mouse(KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2010) Tuzcu, Mehmet; Sur, Emrah; Celik, Ilhami; Oznurlu, Yasemin; Ciftci, Mustafa KemalIn this study detrimental effects of aflatoxin (AF) on the peripheral blood formula and the alpha naphthyl acetate esterase (ANAE) - positivity profile of peripheral blood lymphocytes (PBL) on the mice were determined. A total of 40 white mice were randomly divided in to 5 groups each having 8 animals. The first group served as a control and received standard diet. The remaining 4 groups were fed diets containing 200, 400, 800 and 1600 ppb AF, respectively. The results of this study have revealed that AF given by the food caused significant declines both in the proportion of PBL and in the percentages of ANAE-positive peripheral blood lymphocytes (T-lymphocytes) in a dose dependent manner. These results may be concerned as a haematological evidence for immuno-suppressive effect of AF on cell-mediated immunity.Öğe Effects of High Incubation Temperature on the Body Weight and Yolk Consumption of Two Commercial Broiler Strain(UNIV FED RIO GRANDE DO SUL, 2014) Ozaydin, Tugba; Celik, IlhamiBackground: Temperature is the most important factor controlling embryonic development. Hyper-and hypothermic effects depend on the degree of temperature, duration of exposure and the stage of the development. From the middle of incubation period, embryo temperature is greater than air temperature of the incubator because of the increased metabolic activity. Thus elevated temperature especially in the second half of incubation has a major impact on hatchability and chick quality. The aim of this work was to investigate and compare the effects of high incubation temperature applied from the 10th day to the end of incubation on the body weight and yolk consumption of Ross 308 and Hybro embryos. Materials, Methods & Results: In the experiment, a total of 500 eggs, 250 fertile eggs from each of Hybro and Ross 308 broiler strains were used. The eggs of each strain were weighed and then divided into 2 groups as control and high incubation temperature exposed (heat-stress) groups. The control eggs of both strains were maintained under optimal conditions (incubation temperature: 37.8 degrees C) during the whole incubation period, whereas heat stress imposed eggs were maintained under 37.8 degrees C until the 10th day of incubation and then continuously exposed to high temperature (38.8 degrees C) in the incubator. Other environmental conditions were standardized. At the 11, 13, 15, 18, 20 and 21st days of incubation, randomly sampled eggs from each group were opened until 10 living embryos were obtained from each group. The embryos and their yolk sacs were weighted at the 11, 13, 15, 18 and 20th of incubation and chick weight with yolk sac were determined on hatching day (d 21 of incubation) of the groups. Mean relative embryo weights [(embryo weight/egg weight at setting) x 100] and relative yolk sac weights [(yolk sac weight/egg weight at setting) x 100] of each groups were calculated. In both strain, the heat stress group had a significantly lower yolk-free embryo weight than the control group. However relative yolk weights in heat-stress groups were found to be significantly higher when compared to those in the control groups. There was no significant difference between mean embryo and yolk sac weights of the control groups both strains, except for embryo weight at 20th d of incubation. We also observed that the chicks were heavier at the day of hatch in heat-stress groups than the control groups. Discussion: These observations suggest that high incubation temperature (38.8 degrees C) from the 10th of incubation decreased yolk consumption and depressed mean embryonic weights. Maternal antibodies are passively transferred from the hen to the offspring through the yolk and albumin. Therefore decreased consumption of the yolk due to high incubation temperature will not only affect the body weight, but may also negatively affect the immune status of the newly hatched chick. We also observed that body weight of the chicks at the hatch day was higher in the heat-stress group of both strains in comparison to their controls. Greater amounts of remaining of unused yolk in the heat-stress groups may be responsible for this difference. Results of the present study have revealed that the efforts to increase the body weight of broilers should not be limited to management and care at post hatch period, incubation factors affecting the performance should be determined and taken necessary precautions.Öğe The Effects of Laser with Different Parameters and Crosshatching on Fibroblast Adhesion and Proliferation to Implant Surfaces(QUINTESSENCE PUBLISHING CO INC, 2017) Celebi, Hakki; Arpaci, Pembegul Uyar; Celik, Ilhami; Akman, SerhanPurpose: To compare the adhesion and proliferation effect of HGF-1 cells on pure titanium disks when the surfaces are unprocessed (machined surfaces after slicing) or modified with a laser. Materials and Methods: Twenty-eight titanium disks were divided into four groups. Three surface topographies were created using an erbium fiber laser: group 1 (unidirectional application [no crosshatch]), group 2 (crosshatching in two directions), and group 3 (crosshatching in three directions). The samples in group 4 were unprocessed and served as controls. The surface roughness of samples was investigated with scanning electron microscopy (SEM) and measured with a mechanical surface profilometer. HGF-1 cell line was used to analyze the adhesion of fibroblasts on the sample surfaces. The authors used SEM, XTT, and acridine orange tests to determine the adhesion of HGF-1 onto specimen surfaces and the cell morphology after incubation for 72 hours. XTT results and surface roughness values for all specimens were analyzed using the Kruskal-Wallis test. Results: Ra values of group 1 to group 4 (control) were 1.13, 0.26, 0.38, and 0.19, respectively. Crosshatching decreased the surface roughness values compared with unidirectional application (P = .003). Cell morphology observed in SEM showed that the elongation direction of cells resulted in all directions related with additional filopodia extentions within the crosshatch groups. Therefore, the highest cell viability was also detected in the three-directional crosshatch group (group 3) (P = .000) in XTT assay. According to the acridine orange test, higher cell numbers were seen in group 3, similar to the XTT findings (P = .86). Conclusion: Crosshatching significantly increased the cell-covered implant surfaces compared with the unidirectional group. Decreasing the surface Ra values via crosshatching helped spread the fibroblast over the implants in any direction, thus increasing cell proliferation and adhesion.Öğe Effects of zinc and aprotinin on the healing of ulnar diaphyseal fractures in rabbits(VETERINARNI A FARMACEUTICKA UNIVERZITA BRNO, 2018) Izci, Celal; Celik, Ilhami; Erol, MuharremThe effects of zinc and aprotinin on fracture healing in experimentally induced fractures were investigated by means of histometric analyses and alkaline phosphatase histochemistry. Healthy 54 adult New Zealand White female rabbits were separated into three groups as control, zinc, and aprotinin treatment. The control animals did not receive any medicament; zinc sulphate was given orally to the rabbits in the Zn group for 15 days. Aprotinin was postoperatively infiltrated into the fracture area at the 3rd and 24th h following operation. Immobilization of fracture ends of all groups was similar throughout the experiment. The zinc administered group displayed the highest alkaline phosphatase positive cell level through the experiment. By day 30 after the operation, fibrocartilage and osseous tissues reached the highest levels in the zinc treated group. Based on the observation of augmented osseous tissue formation and increased alkaline phosphatase positive osteoblastic cell activity in the callus, it was conluded that Zn sulphate is a potent stimulator of bone formation by increasing mineralization in the fractured bone segments.Öğe Evaluation of the biocompatibility and osteoproductive activity of ostrich eggshell powder in experimentally induced calvarial defects in rabbits(WILEY, 2008) Durmus, Ercan; Celik, Ilhami; Aydin, M. Faruk; Yildirim, Guelsuen; Sur, EmrahThe purpose of this study was to investigate the beneficial effects of particulate ostrich eggshell grafting on the healing of experimentally induced skull defects. The clinical, radiological, histological, and histomorphometrical findings of this material were compared with the results of commercially available demineralized bone matrix (DBM). The study was conducted on 18 adult New Zealand rabbits. One defect served as a control and the remaining ones either were filled with different sized eggshell particles or DBM, in each animal. Clinical and radiological inspections and histologic investigations of the animals were done at the 1st, 3rd, and 6th months of postoperative period. Radiologically, minimal bone regeneration was observed at the empty, control defect sites. The most advanced bone regeneration was in the DBM grafted defects. The eggshell particle grafted defect sites displayed weak bone regeneration at earlier stages, at 1st and 3rd months after operation when compared with demineralized bone matrix. Nevertheless, ossification was satisfactory at 6th month after operation when compared with the control defects. Within the limitations of this study, it was concluded that Ostrich eggshell powder (OSP) is a worth-while bone substitute because it is a safe, cheap, and easily available material. Long-term studies will clarify its possible role in maxillofacial surgery. Further sophisticated experiments should be undertaken before human implantation concerning its osteoproductive activity alone or in combination with other materials. (C) 2007 Wiley Periodicals, Inc.Öğe Histological and Histochemical Evaluations on the Effects of High Incubation Temperature on the Embryonic Development of Tibial Growth Plate in Broiler Chickens(WILEY, 2016) Oznurlu, Yasemin; Sur, Emrah; Ozaydin, Tugba; Celik, Ilhami; Uluisik, DenizThe effects of experimentally induced high incubation temperature on the embryonic development of growth plate of the chicken were investigated by means of histological and enzyme histochemical methods. In the experiments, 250 fertile eggs of Ross-308 broiler strain were divided into two groups, the control eggs were maintained under optimal conditions (37.8 degrees C and 65% +/- 62% relative humidity, rh) during the whole incubation period. Heat-stress imposed eggs were maintained under normal conditions (37.88C and 65% 62% rh) until the 10th day of incubation, and then, continuously (24 h per day) exposed to high temperature (38.8 degrees C and 65% +/- 62% rh). Tissue samples were taken from 10 animals of each group at the 11th, 13th, 15th, 18th, 21st days of incubation. Tissue samples were processed by enzyme histochemical methods in addition to routine histological techniques. The relative tibia weights and tibia length were lower in the heat-stress group compared to the control group. The results of the measurements of the growth plate showed that the proliferative zone was narrowed whereas, the transitional and hypertrophic zone were thickened in the heat stress group. Alkaline phosphatase (ALP) density was significantly decreased in the heat-stress group compared to the control group. In conclusion, bone formation and growth plate formation are crucial for embryo development and 1 degrees C higher from optimum may increase the incidence of skeletal disorders and leg problems in broiler chickens which is one of the major animal welfare concerns for the poultry industry. Microsc. Res. Tech. 79: 106-110, 2016. (C) 2016 Wiley Periodicals, Inc.Öğe Histological and histomorphometric studies on the cerebellar cortex and silver stained nucleolus organizer regions of Purkinje neurons in chronic morphine-treated rats(UNIV ZAGREB VET FACULTY, 2018) Celik, Ilhami; Seker, Muzaffer; Salbacak, AhmetThe effects of chronic morphine administration on the rat cerebellum and silver stained nucleolus organizer regions in Purkinje cells were investigated by means of histological, histochemical and histometrical techniques. Thirty-two young (30-32 days of age) Wistar rats (equal numbers of both genders) were randomly divided into 2 groups, as control and morphine-treated, each having equal numbers of both genders, a total of 16 animals. The control animals were injected subcutaneously with 1mL/kg physiological saline, and the morphine-treated rats received 5 mg/kg morphine hydrochloride subcutaneously at daily intervals for 30 days. The thickness of the molecular and granular layers of the cerebellum, the diameters of the nuclei and nucleoli of cerebellar Purkinje neurones, and the number and size of the silver staining nucleolus organizer regions of the Purkinje cell nuclei were determined histomorphometrically. The morphine administration caused slight histological changes in the cerebellum. The molecular layer thickness of the cerebellum was significantly (P<0.05) reduced, and the decrease was mainly in the male animals. In contrast, the layer thickened insignificantly in the morphine-treated females. The granular layer thickened slightly but insignificantly in both genders of the morphinetreated group. The Purkinje cell count significantly decreased with morphine treatment. Nucleus size did not change with morphine treatment. However, morphine-treated animals had smaller nucleoli. It was concluded that morphine treatment caused significant histomorphological changes in the cerebellar cortex in a sexually dimorphic manner.Öğe Histological evaluation of the effects of bioglass, hydroxyapatite, or demineralized freeze-dried bone, grafted alone or as composites, on the healing of tibial defects in rabbits(SAUDI MED J, 2009) Kucukkolbasi, Hasan; Mutlu, Necip; Isik, Kubilay; Celik, Ilhami; Oznurlu, YaseminObjectives: To compare the effectiveness of bioactive glass (BG), natural hydroxyapatite (HA), and demineralized freeze-dried bone (DFDB) in bone defects. Methods: All animal experiments were conducted in Faculty (if Veterinary Medicine, Selcuk University, Konya, Turkey, under the Selcuk University Guidelines for Animal Experimentation, in 2005. Eighteen New Zealand rabbits were used for the experiment. Four cavities were prepared on right and left tibias. The cavities on the right tibia were filled with either BG, HA or DFDB. One cavity was left empty as a control. The cavities on the other tibia] bone were grafted with HA+BG, DFA+DFDB, BG+DFDB and HA+BG+DFDB composites. Histological examinations were performed at first, third, and sixth postoperative months. Results: According to histomorphometric findings, the mixture containing HA+BG+DFDB obtained the best histological results (P<0.05). Conclusion: The composite graft of HA, BG and DFDB is more effective than when used as individual agents.Öğe Histological, Histochemical and Immunohistochemical Investigations on the Developing Small Intestines of Broiler Embryos(Medwell Online, 2012) Ozaydin, Tugba; Celik, IlhamiThe aim of the present study was to investigate comparatively embryonic development of the small intestine in two boiler strains by means of histological, histochemical and immunohistochemical methods. In the experiments, 125 fertile eggs from each of two commercial broiler strains were used. On the days 11, 13, 15, 18, 20 and 21 of incubation, intestinal tissue samples were taken from 10 live embryos of each strain. Rapid histological, histochemical and immunohistochemical changes occurred in the small intestine during the investigation period. Although, the intestinal villi were rudimentary and uniform on the days 11, 13 and 15, they were at different developmental stages on the days 18, 20 and 21 of incubation. Mucin containing goblet cells first appeared at 13th day of incubation. Majority of the goblet cells contained acidic mucin at 18th day of incubation. Goblet cell density increased on the days 20 and 21 of incubation. Enteroendocrine cells were first seen at 13th day of incubation. Their number was 1-4 in the majority of the villi on the day 18 of incubation and remained relatively constant thereafter. Most of the villous epithelial cells were proliferative cells. A few apoptotic cells were also observed towards the apex of the villi on the days 20 and 21 of incubation. Intestinal development patterns of both strains were quite similar. When considering the importance of embryonic development of small intestines to the chick growth and development, the data may contribute to comparative studies on the performance traits of these broiler strains.Öğe Immunohistochemical Distribution of Heat Shock Protein 70 and Proliferating Cell Nuclear Antigen in Mouse Placenta at Different Gestational Stages(WILEY, 2016) Ozaydin, Tugba; Sur, Emrah; Oznurlu, Yasemin; Celik, Ilhami; Uluisik, DenizThe aim of the present study was to investigate immunohistochemical distribution of heat shock protein 70 (Hsp70) and proliferating cell nuclear antigen (PCNA) in the mouse placenta at different gestational stages. For this purpose a total of 18 Swiss albino female mice at 12-14 weeks of age were used. Females were sacrificed on days 3 (early), 10 (mid-), and 17 (late) of pregnancy and the implantation sites of the pregnant uterus were sampled. The sections were made transversely through the central region of the implantation site and stained with hematoxylin and eosin for histological examination. PCNA and Hsp70 was stained immunohistochemically. Since the definitive placenta was not still formed on day 3 of pregnancy, Hsp70 and PCNA positivity were evaluated in only luminal epithelium and decidual-stromal cells. On days 10 and 17 of pregnancy, Hsp70 and PCNA positivity were evaluated in labyrinth zone, junctional zone and decidual layer of placenta. Hsp70 expression was observed trophoblast cells and decidual cells and was relatively constant throughout the pregnancy. This protein was strongly labeled in the trophoblast cells; while decidual cells were displayed moderate staining. In early pregnant mouse uteri, PCNA was mainly localized in decidual-stromal cells. The trophoblast cells and decidual cells displayed highly proliferative activity at the midgestational period. However there was a significant decrease in the percentage of PCNA positive cells in late gestation. (C) 2016 Wiley Periodicals, Inc.Öğe Light microscopic evaluation of the nucleolar organiser regions of skin cells of the dogs subjected to therapeutic ultrasounds(NATL VETERINARY RESEARCH INST, 2008) Ogurtan, Zeki; Aydin, Mehmet Faruk; Ceylan, Cengiz; Celik, IlhamiA possible use of some argyrophilic nucleolar organiser region (AgNOR) parameters as a biological counter to predict the biological behaviour of basal epidermal cells and dermal fibroblasts in dogs subjected to therapeutic ultrasound (US) exposure was determined. All the dogs, except the control dogs, had US treatment at an intensity of 0.5 W/cm(2) (SATA), frequency of 1.0 MHz, pulsed 2 ms on and 8 ms off for 5 min daily for 10 d. Full thickness skin samples were taken on days 4, 18, 32, 46, and 74 following cessation of 10 d US application and right after the 10(th) d of US exposure. A statistically significant difference immediately at the end of day 10 of US exposure was found for all parameters measured, except NOR diameters of the stratum basale cells. All the measured parameters, except NOR numbers of fibroblasts in the dermis, returned to normal (control) values by day 74 indicating that the therapeutic effect of US at the given characteristics lasts at least longer than 46 d regarding thickness of the stratum corneum, longer than 32 d regarding NOR numbers in the cells of the stratum basale, and NOR diameters in the cells of the stratum basale, and longer than 74 d regarding NOR numbers in fibroblasts. The results presented herein suggest that there is a correlation between the longevity of ultrasound effect and the alteration of AgNORs parameters and their characteristics. Considering the longer duration of reversible alterations in our study, it would be reasonable to determine the effectiveness of ultrasound by AgNOR counting.Öğe Light microscopic investigations on the circumvallate papillae of the young and aged akkaraman sheep(KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2012) Unsal, Sadettin; Cuce, Hasan; Celik, Ilhami; Sur, Emrah; Ozparlak, HalukIn this light microscopic study, morphometric parameters of the circumvallate papillae and the number of their taste buds in tongues of young (6-9 month-old) and aged (7 year-old) Akkaraman sheep were compared in order to determine general morphology and whether there were age-related differences in general morphology and numbers of the taste buds between young and aged animals. The tongues were obtained from a local slaughterhouse. Tissue samples containing dorsal lingual papillae of 32 tongues (16 young and 16 aged) from both sexes (equal numbers of the males and females) were used for histological investigation. The mean number and diameter of the circumvallate papilla, mean number and diameter of the taste buds, and the taste bud number per circumvallate papilla were determined under the light microscope. There were no significant differences between the young and aged animals in the mean number and diameter of circumvallate papillae, the taste bud number and the diameter, taste bud number per papilla. Nevertheless, the aged animals had larger circumvallate papillae. These results showed that number of the taste buds and circumvallate papilla did not change with aging in Akkaraman sheep. Because that general morphology of the circumvallate papillae and their taste bud numbers did not change even in older animals, any age-related differences in the sense of taste can not be attributed to gross degenerative changes in taste buds of circumvallate papillae.Öğe The proportion and the distribution of t-lymphocytes, null lymphocytes and acid phosphatase positive lymphocytes of the peripheral blood and endometrium in pregnant mice(KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2014) Sur, Emrah; Oznurlu, Yasemin; Ozaydin, Tugba; Celik, Ilhami; Aydin, Ibrahim; Kadiralieva, NaristeThis study was performed to determine the effects of pregnancy on the proportion of T-lymphocytes, null lymphocytes and acid phosphatase (ACP-ase) positive peripheral blood lymphocytes and the distribution of the mentioned cells in the decidua basalis region of endometrium in the pregnant mice. For this purpose, mice at 12-14 weeks of age were divided into four groups as non-pregnant control, and at the middle of the first, second, and the third week of the pregnancy, corresponding to early, middle, and late (3rd, 10th, and 17th days of pregnancy) gestational stages respectively (n = 6 for each group). The lowest T-lymphocytes percentage was determined at early and middle pregnancy in the peripheral blood (43.83%) and decidua basalis (10.83 number/0.1 mm(2)), respectively. There was a statistically significant increase in the proportions of the peripheral blood ACP-ase (+) lymphocytes (44.33%) at the mid-gestational period while the lowest ACP-ase positive lymphocyte numbers (5.50 number/0.1 mm(2)) in the decidua basalis was observed at early pregnancy. The highest null cell rates were found at early gestation either in the peripheral blood (11.50%) or in the decidua basalis (7.83 umber/0.1 mm(2)). The lowest percentage of peripheral blood lymphocyte (56.00%) was recorded at the early pregnancy. It was concluded that the number and the distribution of the lymphocyte, T-lymphocyte, null lymphocyte, and ACP-ase positive lymphocyte were affected by pregnancy although there were some differences among the gestational periods.Öğe A STUDY OF PERIPHERAL BLOOD IN HEDGEHOGS IN TURKEY(AMER ASSOC ZOO VETERINARIANS, 2011) Ozparlak, Haluk; Celik, Ilhami; Sur, Emrah; Ozaydin, Tugba; Arslan, AtillaThe aim of this study was to determine diameters of blood cells, differential counts of peripheral blood leukocytes, alpha-naphthyl acetate esterase (ANAE), acid phosphatase (ACP-ase) activity of some leukocyte types, and enzymatic positivity percentages of peripheral blood lymphocytes in two hedgehogs species, Hemiechinus auritus, the long-eared hedgehog, and Erinaceus concolor, the southern white-breasted hedgehog. Air-dried peripheral blood smears were stained with May-Grunwald-Giemsa stain. ANAE and ACP-ase were stained in glutaraldehyde-acetone-fixed smears. ANAE-positive lymphocytes displayed a dot-like positivity pattern characterized with 1-5 reddish brown cytoplasmic granules, whereas ACP-ase positive lymphocytes displayed a dot-like positivity pattern characterized with 1-3 pinkish cytoplasmic granules. Monocytes gave a diffuse and strong reaction while neutrophils displayed a weak positive reaction for ANAE and ACP-ase. No difference was observed in mean diameters of peripheral blood cells of these species. It was found that lymphocytes made up the majority (64.3% and 65.5%) of leukocytes, followed by neutrophils (23.9% and 23.3%), eosinophils (9.0% and 7.6%), monocytes (1.8% and 2.3%), and basophils (1.0% and 1.3%) in H. auritus and E. concolor, respectively. Mean ANAE positivity of lymphocytes was 36.6% and 51.3% and ACP-ase positivity was 32.1% and 37.5% for H. auritus and E. concolor, respectively. The ANAE positivity of lymphocytes in E. concolor was significantly (P < 0.05) higher than that of H. auritus.