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Anthraquinone profile, antioxidant and enzyme inhibitory effect of root extracts of eight Asphodeline taxa from Turkey: can Asphodeline roots be considered as a new source of natural compounds?
(TAYLOR & FRANCIS LTD, 2016) Zengin, Gökhan; Locatelli, Marcello; Ceylan, Ramazan; Aktümsek, Abdurrahman
Plant-based foods have become attractive for scientists and food producers. Beneficial effects related to their consumption as dietary supplements are due to the presence of natural occurring secondary metabolites. In this context, studies on these products are important for natural and safely food ingredients evaluation. The aim of this study was to evaluate root extract of eight Asphodeline species as antioxidants, enzyme inhibitors and phytochemical content. Spectrophotometric antioxidant and enzyme inhibitory assays were performed. Total phenolic and flavonoids contents as well as the chemical free-anthraquinones profiles were determined using routinely procedure (HPLC-PDA). Data show that Asphodeline roots can be considered as a new source of natural compounds and can be used as a valuable dietary supplement. Some differences related to biological activities can be inferred to other phytochemicals that can be considered in the future for their synergic or competitive activities.
Antioxidant Potential and Inhibition of Key Enzymes Linked to Alzheimer's Diseases and Diabetes Mellitus by Monoterpene-Rich Essential Oil from Sideritis galatica Bornm. Endemic to Turkey
(ACG PUBLICATIONS, 2016) Zengin, Gökhan; Sarıkürkcü, Cengiz; Aktümsek, Abdurrahman; Ceylan, Ramazan
The present study was designated to (1) characterize the essential oil from S. galatica (SGEOs) and (2) evaluate its antioxidant and enzyme inhibitory activities. Antioxidant capacity was tested different methods including free radical scavenging (DPPH, ABTS and NO), reducing power (FRAP and CUPRAC), metal chelating and phosphomolybdenum. Inhibitory activities were analyzed on acetylcholiesterase, butrylcholinesterase, alpha-amylase and alpha-glucosidase. SGEOs were chemically analyzed and identified by gas chromatography and gas chromatography/mass spectrophotometry. 23 components, representing 98.1% of SGEOs were identified. Monoterpene hydrocarbons (74.1%), especially alpha-(23.0%) and beta-pinene (32.2%), were the main constituents in SGEOs. The main sesquiterpene hydrocarbons were beta-caryophyllene (16.9%), germacrene-D (1.2%) and caryophyllene oxide (1.2%), respectively. Generally, SGEOs has shown moderate free radical, reducing power, metal chelating and enzyme inhibitory activities. These activities related to chemical profile in SGEOs. Our findings supported that the possible utility of SGEOs is a source of natural agents for food or pharmaceutical industries.
Bazı sıderıtıs ekstreleri kullanılarak yeşil sentez ile tekli ve hibrid nanomateryallerinin sentezi ve bunların biyolojik aktiviteleri
(Selçuk Üniversitesi Fen Bilimleri Enstitüsü, 2020) Ceylan, Ramazan; Aktümsek, Abdurrahman
Nanoteknoloji alanı çoğunlukla biyoloji, fizik, kimya ve malzeme bilimlerini kapsar, ancak biyomedikal ve farmasötik uygulamalar için yeni terapötik nanomalzemeler geliştirir. Nanopartiküllerin sentezi fiziksel, kimyasal ve biyolojik veya yeşil sentez yöntemleriyle gerçekleştirilir. Nanopartiküllerin fiziksel ve kimyasal tekniklerle sentezi oldukça pahalıdır ve çevreye potansiyel olarak zararlı olduğu bilinmektedir. Son zamanlarda, bitki ekstreleri kullanılarak metal nanopartiküllerin biyosentezi üzerine yapılan çalışmalar önemli ölçüde artmıştır. Bitki metabolitleri çevre dostu bir şekilde metalik nanopartiküllerin üretimini uyarır. Bitki kaynaklı nanopartiküllerin farmasötikler, terapotikler, sürdürülebilir ve yenilenebilir enerjiler gibi çeşitli alanlarda kullanılabilme potansiyeli oldukça yüksektir. Gelecekte bitkiler, sağlık ve ticari ürünlerde metalik nanopartiküllerin sentezi için oldukça geniş bir perspektife sahiptir. Bu çalışmada üç Sideritis (S. argyrea, S. brevidens ve S. lycia) türü kullanılarak, yeşil sentez ile Au, Ag, Fe metal NP'leri ve Cu hibrid nanoyapıları sentezlenmiştir. Sentezlenen bu yapıların karakterizasyonu UV-Vis, FTIR, SEM, TEM, XRD ve EDX ile gerçekleştirilmiştir. Yeşil sentez ile sentezlenen bu yapıların global sağlık problemlerinden olan Alzheimer, diabet ve deri hastalıkları ile ilişkili enzimlerin inhibisyon testleri gerçekleştirildi. Au NP'leri tüm örnekler içinde en iyi BChE ihibisyon aktivitesine sahiptir (12.57-11.66 mgGA/g). Tüm örnekler içerisinde KA FeNP (866.38 mgAKA/g) en yüksek glukozidaz inhibisyonu aktivitesine sahiptir. Elde edilen sonuçlar, nanoparçacıkların ve hibrid nanoyapıların önemli enzim inhibitör aktivitesinin farmasötik alanlara uygulanabileceğini göstermektedir.
Biological activities of three extracts from artedia squamata: A study on antioxidant and enzyme inhibitory potential
(Bentham Science Publishers B.V., 2015) Zengin, Gökhan; Ceylan, Ramazan; Uysal, Şengül; Aktümsek, Abdurrahman
The present work focused on antioxidant and enzyme inhibitory potential of different solvent extracts (ethyl acetate, methanol and water) from Artedia squamata. Cholinesterase, tyrosinase, ?-amylase and ?-glucosidase inhibitory activities of the extracts were tested by microtitre plate assays. Antioxidant abilities were tested using free radical scavenging (DPPH and ABTS), reducing power (FRAP and CUPRAC), total antioxidant capacity and ferric ion chelating assays. The total phenolic contents of these extracts ranged from 26.24 to 35.16 mg GAEs/g extract. Methanol and water extracts showed higher phenolic content, DPPH and ABTS scavenging activity and reducing power activities, while ethyl acetate extract had highest chelating and inhibition abilities on AChE, ?-amylase and ?- glucosidase. With regard to tyrosinase inhibitory activity, water extract showed a good inhibitory activity with 11.91 mg KAEs/g. Present study showed that Artedia squamata can be considered as good source of natural agents for food and drug applications. © 2015 Bentham Science Publishers.
Changes in Coal Composition During Air Oxidation at Moderate Temperature
(TAYLOR & FRANCIS INC, 1989) Yılmaz, Mustafa; Ceylan, Ramazan; Kara, Hüseyin
In this work, under atmospheric pressure and moderate temperature for various periods, e.g., 30, 45, 90, and 135 days, the air oxidation of two Central Anatolian lignites were examined. The structural changes of the lignite samples were observed by analyzing the amount of phenolic hydroxyl, carboxyl, andperoxide of the samples oxidized and by comparing the IR spectra of the lignites, both original and oxidized. It was concluded that most of the phenolic structures of the lignites were oxidized into quinonoid structures during the oxidation process.
Chemical and biological fingerprints of two Fabaceae species (Cytisopsis dorycniifolia and Ebenus hirsuta): Are they novel sources of natural agents for pharmaceutical and food formulations?
(ELSEVIER SCIENCE BV, 2016) Ceylan, Ramazan; Katanic, Jelena; Zengin, Gökhan; Matic, Sanja; Aktümsek, Abdurrahman; Boroja, Tatjana; Stanic, Snezana
In the present study, the methanolic extracts of Cytisopsis dorycniifolia and Ebenus hirsuta were analyzed for antioxidant, enzyme inhibitory, antimicrobial and antigenotoxic activities. The identification and quantification of phenolic compounds in these extracts were performed using liquid chromatography coupled with mass spectrometry (LC-MS/MS) technique, and the main components were hyperoside, rutin, hesperidin, tannic acid and p-coumaric acid. The extracts had remarkable biological effects. Generally, C. dorycniifolia had higher level of bioactive components and exhibited stronger in vitro antioxidant effects than E. hirsuta. E. hirsuta was active especially against bacteria Pseudomonas fluorescens and Agrobacterium tumefaciens and fungi Puncturella fastigiata, while C dorycniifolia was active against bacteria E. faecalis and had moderate activity against Trichoderma species. The extracts displayed strong antigenotoxic effects (98.6% for E. hirsuta and 76.3% for C dorycniifolia). Our findings suggested that C dorycniifolia and E. hirsuta could be beneficial for the preparation of high-value pharmaceuticals and functional food ingredients. (C) 2016 Elsevier B.V. All rights reserved.
Combining in vitro, in vivo and in silico approaches to evaluate nutraceutical potentials and chemical fingerprints of Moltkia aurea and Moltkia coerulea
(PERGAMON-ELSEVIER SCIENCE LTD, 2017) Zengin, Gökhan; Ceylan, Ramazan; Katanic, Jelena; Mollica, Adriano; Aktümsek, Abdurrahman; Boroja, Tatjana; Matic, Sanja
Methanolic extracts of Moltkia aurea Boiss. (MA) and Moltkia coerulea (Wild.) Lehm. (MC) were investigated for their antioxidant capacity and enzymatic inhibitory potential against acetylcholinesterase, butyrylcholinesterase, alpha-amylase, alpha-glucosidase, and tyrosinase in vitro. MA and MC were also explored for their antimicrobial effect, as well as for their possible genotoxic/antigenotoxic potential on Drosophila melanogaster in vivo. The total bioactive components (phenolic (TPC) and flavonoid contents (TFC)) were determined and liquid chromatography-tandem mass spectrometry (LC-MS/MS) metabolite profiling of MA and MC appraised. The plausible docking poses of bioactive compounds to key enzymes were further studied using molecular modelling approach. MA proved to be a better antioxidant with higher TPC and TFC compared to MC. Protocatechuic acid, rutin, hesperidin and malic acid were the most abundant in these extracts. Both MA and MC exhibited antigenotoxic potential with a %R in DNA damage of 60.90 and 53.14% respectively. The docking studies revealed that rutin, hesperidin, and rosmarinic acid have the best scores for all the enzymes tested. MA and MC were found to be rich in phytochemicals with potent antioxidant, antimicrobial, and antigenotoxic activities that can be further studied for the management of neurodegenerative complications, diabetes, and hyperpigmentation. (C) 2017 Elsevier Ltd. All rights reserved.
A Comparative fatty acid compositional analysis of different wild species of mushrooms from Turkey
(UNITED ARAB EMIRATES UNIV, 2015) Zengin, Gökhan; Sarıkürkcü, Cengiz; Aktümsek, Abdurrahman; Uysal, Şengül; Ceylan, Ramazan; Anwar, Farooq; Solak, Mehmet Halil
Mushrooms have been recognized as important food items due to their high nutritional and medicinal value. The present work quantifies and compares the fatty acids composition among eleven mushroom species (Auricularia auricular-judae, Collybia dryophila, Rammulina ve/utipes, Helvella lacunose, Polyporus squamosus, Rhizopogon roseolus, Russula albonigra, Russula delica, Sparassis crispa, collinitus and Volvariella gloiocephala) harvested from Turkey. The lipid contents of the mushrooms varied from 0.13% (Auricularla auricula-judae) to 2.90% (Sparassis crispa). A total of 14 fatty acids were identified and quantified by gas chromatography in the selected mushrooms species with linoleic (13.17 to 79.41%), oleic (0.71 to 55.07%) and palmitic (8.95 to 30.84%) acids as the major components in the tested mushrooms species. The amount of total saturated fatty acids (SFAs), total monounsaturated FAs (MUFAs) and total polyunsaturated FAs (PUFAs) ranged from 13.97 to 59.44%, 0.94 to 55.44% and 13.54 to 79.93%, respectively. Moreover, the mushrooms tested can be explored as a rich dietary source of essential fatty acids (13.34-79.76%) for human nutrition.
A comparative study of Bulgarian and Turkish Asphodeline lutea root extracts: HPLC-UV profiles, enzyme inhibitory potentials and anti-proliferative activities against MCF-7 and MCF-10A cell lines
(ELSEVIER SCIENCE BV, 2015) Lazarova, Irina; Zengin, Gökhan; Bender, Onur; Zheleva-Dimitrova, Dimitrina; Uysal, Şengül; Ceylan, Ramazan; Gevrenova, Reneta
Asphodeline lutea (L.) Rchb. is a wild plant traditionally used as a food in the Mediterranean region. The alcoholic extracts of A. lutea roots from different origins (Bulgarian and 'Rakish) were investigated for anti-cholinesterase, anti-tyrosinase, anti-amylase, anti-glycosidase and anti-proliferative activity (against MCF-7 and MCF-10A cell lines). A validated HPLC method for the determination of two anthraquinones, two bianthraquinones, two naphthalenes, two flavonoids and one hydroxycinnamic acid was developed due to the presence of these components in the genus Asphodeline. In addition, total anthraquinones content was determined spectrophotometrically. A. lutea from Bulgaria demonstrated higher level of total anthraquinones (0.81 +/-0.05 mg g(-1)). A naphthalene derivative (2-acetyl-1,8-dimethoxy-3-methylnaphthalene) was the major compound in Bulgarian accession (0.96 +/- 0.03 mg g(-1)), while caffeic acid was the main analyte in Turkish sample (0.42 +/- 0.06 mg g(-1)). Turkish extracts were more potent as cholinesterase and tyrosinase inhibitors but less active as antidiabetic agent. The higher anti-proliferative effect was presented by Bulgarian extract with IC50 values 120 +/- 16.8 mg mL(-1) (for MCF-7) and 256 +/- 16.1 mg mL(-1) (for MCF-10A). (C) 2015 Elsevier Ltd. All rights reserved.
Composition, antioxidant, antimicrobial and enzyme inhibition activities of two Origanum vulgare subspecies (subsp vulgare and subsp hirtum) essential oils
(ELSEVIER SCIENCE BV, 2015) Sarıkürkcü, Cengiz; Zengin, Gökhan; Oskay, Mustafa; Uysal, Şengül; Ceylan, Ramazan; Aktümsek, Abdurrahman
Essential oils (EOs) from Origanum vulgare subsp. vulgare (OW) and O. vulgare subsp. hirtum (OVH) were evaluated for antioxidant activities (DPPH, ABTS, FRAP, CUPRAC, beta-carotene/linoleic acid, phospho-molybdenum and metal chelating), antimicrobial and inhibitory properties against acetylcholinesterase, butrylcholinesterase, tyrosinase, alpha-amylase and alpha-glucosidase. Thymol and linalool were identified as major component in OW and OVH essential oils, respectively. O. vulgare subsp. vulgare exhibited a strong free radical scavenging, reducing power, antimicrobial and acetylcholinesterase, butrylcholinesterase, alpha-amylase and alpha-glucosidase inhibitory activity. Interestingly, OVH possess a significant metal chelating and tyrosinase inhibitory activities. The EOs of both species exhibited moderate antibacterial and antifungal activities. The MIC values of the EOs ranged from 85.3 to 426.7 mu g/mL. O. vulgare subsp. vulgare showed higher activity against Sarcina lutea with the lowest MIC (85.3 mu g/mL), whereas OVH indicated strong activity for Candida albicans with MIC value of 85.3 mu g/mL. The data suggest that the Origanum EOs could be used as valuable new natural agents with functional properties for food and pharmacology industries. (C) 2015 Elsevier B.V. All rights reserved.
A comprehensive study on phytochemical characterization of Haplophyllum myrtifolium Boiss. endemic to Turkey and its inhibitory potential against key enzymes involved in Alzheimer, skin diseases and type II diabetes
(ELSEVIER SCIENCE BV, 2014) Zengin, Gökhan; Sarıkürkcü, Cengiz; Aktümsek, Abdurrahman; Ceylan, Ramazan; Ceylan, Olcay
The interest of medicinal plants as therapeutic agents against diseases such as Alzheimer, skin disorders and diabetes mellitus is growing. In order to investigate the influence of Haplophyllum myrufolium, four solvent extracts (petroleum ether, ethyl acetate, methanol and water) were screened for antioxidant potentials, anti-cholinesterase, anti-tyrosinase, anti-amylase and anti-glycosidase activity. Antioxidant effects were elucidated by different assays including free radical scavenging (ABTS, DPPH and NO assay), reducing power (FRAP and CUPRAC), phosphomolybdenum, beta-carotene/linoleic acid system and metal chelating. Total phenolic, flavonoid, flavanol, tannin and saponin contents in these extracts were also calculated. Generally, ethyl acetate extract showed the strongest antioxidant activity in these extracts. This activity may be related to a good total phenolic content. The total phenolic contents of these extracts ranged from 32.32 to 52.50 mg GAEs/g extract. Except for water extract, all extracts showed good inhibitory activities on cholinesterase, tyrosinase, amylase and glycosidase. Apolar extracts (petroleum ether and ethyl acetate) were more potent as enzyme inhibitors in comparison to polar extracts (methanol and water). These findings reveal that H. myrtifolium could be used a source of natural compounds for the management of oxidative damage, Alzheimer, skin disorders and diabetes mellitus. (C) 2014 Elsevier B.V. All rights reserved.
Crepis foetida L. subsp rhoeadifolia (Bleb.) Celak. as a source of multifunctional agents: Cytotoxic and phytochemical evaluation
(ELSEVIER SCIENCE BV, 2015) Zengin, Gökhan; Sarıkürkcü, Cengiz; Uyar, Pembegül; Aktümsek, Abdurrahman; Uysal, Şengül; Koçak, Mehmet Sefa; Ceylan, Ramazan
The cytotoxic, anticholinesterase, antityrosinase and antioxidant effects of methanol extract from the flower of C. foetida subsp. rhoeadifolia (CFRME) were evaluated. The cytotoxic effect was investigated using HepG2, Caco-2, MCF-7 and MCF-10A cell lines. Enzyme inhibitory activities were tested by spectrophotometric methods. Different chemical assays were used to determine the antioxidant effects. In addition, phenolic constituents were quantified. High-performance liquid chromatographic (HPLC) analysis revealed that chlorogenic acid was the major phenolic component in the extract. The extract showed a strong antiproliferative, antioxidant, and anticholinesterase and antityrosinase effects. These findings suggest that C. foetida subsp. rhoeadifolia may be considered as a source of ingredients that can be used as food supplements. (C) 2015 Elsevier Ltd. All rights reserved.
Enzyme inhibitory effect and antioxidant properties of astragalus lagurus extracts
(Bentham Science Publishers B.V., 2016) Zengin, Gökhan; Ceylan, Ramazan; Güler, Gökalp Özmen; Carradori, S.; Uysal, Şengül; Aktümsek, Abdurrahman
Antioxidant and enzyme inhibitory effects (against cholinesterase, tyrosinase, ?-amylase and ?-glucosidase) of ethyl acetate, methanol, and aqueous extracts from Astragalus lagurus (Fabaceae) were investigated with spectrophotometric methods. Antioxidant capacity were evaluated by using different assay including free radical scavenging (DPPH and ABTS), reducing power (FRAP and CUPRAC), phosphomolybdenum, and metal chelating experiments. Total phenolic and flavonoid content was also determined as 20.34-20.72 mg GAEs/g and 19.58-31.10 mg REs/g, respectively. Generally, A. lagurus ethyl acetate and aqueous extracts were observed to possess higher antioxidant and enzyme inhibitory effects compared to methanol extract. The aqueous extract exhibited the strongest free radical scavenging activity on DPPH (20.65 mg TEs/g) and ABTS (67.43 mg TEs/g) as well as on reducing power tests (73.98 mg TEs/g for CUPRAC and 53.49 mg TEs/g for FRAP). ş-Amylase and ?-glucosidase inhibition were found to be 0.12-0.56 and 1.45-1.82 mmol ACAEs/g. These findings suggest that A. lagurus could provide natural biologically active agents to be used in food and pharmaceutical industry. © 2016 Bentham Science Publishers.
Enzyme Inhibitory Properties, Antioxidant Activities, and Phytochemical Profile of Three Medicinal Plants from Turkey
(HINDAWI LTD, 2015) Zengin, Gökhan; Güler, Gökalp Özmen; Aktümsek, Abdurrahman; Ceylan, Ramazan; Picot, Carene Marie Nancy; Mahomoodally, ‪Mohamad Fawzi
We aimed to investigate the inhibitory potential of three medicinal plants (Hedysarum varium, Onobrychis hypargyrea, and Vicia truncatula) from Turkey against key enzymes involved in human pathologies, namely, diabetes (alpha-amylase and alpha-glucosidase), neurodegenerative disorders (tyrosinase, acetylcholinesterase, and butyrylcholinesterase), and hyperpigmentation (tyrosinase). The antioxidant potential, phenolic and flavonoid content of ethyl acetate, and methanolic and aqueous extracts were investigated using in vitro assays. The total antioxidant capacity (TAC), beta-carotene/linoleic acid bleaching activity, 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH center dot), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS(center dot+)), cupric ion reducing antioxidant capacity (CUPRAC), ferric reducing antioxidant power (FRAP), and metal chelating activity on ferrous ions were used to evaluate the antioxidant capabilities of the extracts. The half-maximal inhibitory concentrations (IC50) of the extracts on cholinesterase, tyrosinase, and alpha-amylase were significantly higher than the references, galantamine, kojic acid, and acarbose, respectively. The half-maximal effective concentrations (EC50) of the extracts on TAC, CUPRAC, and FRAP were significantly higher than trolox. The phenol and flavonoid contents of the plant extracts were in the range 20.90 +/- 0.190-83.25 +/- 0.914mg gallic acid equivalent/g extract and 1.45 +/- 0.200-39.71 +/- 0.092mg rutin equivalent/g extract, respectively. The plants were found to possess moderate antioxidant capacities and interesting inhibitory action against key enzymes.
Ereğli (Konya, Türkiye) Bölgesinden Bal Arılarının Topladığı Polenin Etanol Ekstraktının Antioksidan ve in Vitro Bazı Enzim İnhibitör Aktiviteleri
(Selçuk Üniversitesi Fen Fakültesi, 2017) Özparlak, Haluk; Zengin, Gökhan; Ceylan, Ramazan
Arı poleni bal arıları tarafından granüller şeklinde paketlenen ve daha sonra insanlar tarafından kovanlardan hasat edilen en saf ve en zengin doğal besin takviyelerinden biridir. Bal arılarının topladığı polen, proteinler, aminoasitler, karbohidratlar, lipidler, vitaminler, mineral maddeler gibi besleyici temel unsurlardan oluşur. Polenin sağlık üzerindeki olumlu etkisi fenolik bileşiklere sahip olmasından dolayı antioksidan aktivitesine bağlanmaktadır. Son yıllarda arı poleniyle ilgili çok sayıda araştırma yayınlanmıştır. Arı poleni ve diğer arı ürünleri üzerinde birçok araştırma yapılmış olsa da, enzim inhibisyonu üzerine çok az çalışma bulunmaktadır. Bu nedenle, bu araştırmanın amacı Ereğli (Konya, Türkiye) bölgesinden bal arılarının topladığı karışık polenin etanol ekstraktının total fenolik ve flavonoid içeriklerini ve antioksidan aktivitesini, ayrıca bu ekstraktın kolinesteraz, amilaz ve glukozidaza karşı enzim inhibitör potansiyellerini ilk kez tespit etmektir. Bu çalışmada rutin çalışmalardan farklı olarak ultrasonik ekstraksiyon yöntemi kullanıldı. Ekstrakttaki total fenolik ve flavonoid içerikleri de Folin-Ciocalteu ve AlCl3 yöntemleriyle tespit edildi. Antioksidan aktiviteleri serbest radikal süpürme (DPPH ve ABTS), indirgeme gücü (FRAP ve CUPRAC), fosfomolibdat ve metal şelatlama testleri gibi farklı yöntemlerle araştırıldı. In vitro enzim inhibitör potansiyelleri bir mikroplate okuyucuyla ölçüldü. Aktiviteler standart eş değerler olarak değerlendirildi. Bu çalışmanın sonuçları göstermiştir ki, i. antioksidan aktivitesi açısından, Ereğli bölgesinden elde edilen arı poleni sağlığa yararlı formülasyonlarda ileride kullanmak için değerli fonksiyonel içeriğiyle doğal bir kaynak olarak düşünülebilir, ii. bu bölgenin poleni, Alzheimer ve Diabetes mellitus gibi önemli sağlık problemlerinin tedavisi için doğal bir enzim inhibitörü kaynağı olarak göz önüne alınabilir
Essential Oil Composition of an Uninvestigated Centaurea Species from Turkey: Centaurea patula DC.
(TAYLOR & FRANCIS LTD, 2016) Zengin, Gökhan; Aktümsek, Abdurrahman; Boğa, Mehmet; Ceylan, Ramazan; Uysal, Şengül
Centaurea patula is widely distributed in the Central Anatolia Region of Turkey. The essential oil of C. patula was obtained by hydrodistillation and analyzed by GC-FID and GC-MS techniques. Twenty-one components were identified representing 86.4% of the oil. Spathulenol (14.6%), n-hexadecanoic acid (13.4%), 1-pentadecene (13.1%) and phytol (12.4%) were found to be the major components in this oil. This work is the first report on the chemical composition of the essential oil of C. patula. The results could be valuable for the Centaurea genus in terms of essential oil information's.
Evaluation of Antioxidant Potential, Enzyme Inhibition Activity and Phenolic Profile of Lathyrus Cicera and Lathyrus Digitatus: Potential Sources of Bioactive Compounds for the Food Industry
(ELSEVIER, 2017) Llorent-Martínez, Eulogio J.; Ortega-Barrales, Pilar; Zengin, Gökhan; Mocan, A.; Simirgiotis, M. J.; Ceylan, Ramazan; Uysal, Şengül
The genus Lathyrus has great importance in terms of food and agricultural areas. In this study, the in vitro antioxidant activity (phosphomolybdenum, DPPH, ABTS, FRAP, CUPRAC and metal chelating) and enzyme inhibitory activity evaluation (acetylcholinesterase, butyrylcholinesterase, alpha-amylase and alpha-glucosidase) of L. cicera and L. digitatus were investigated, as, well as their phytochemical profiles. The screening of the main phytochemical compounds in aerial parts of L. cicera and L. digitatus was carried out by high-performance liquid chromatography with electrospray ionization mass spectrometric detection (HPLC-ESI-MSn), observing that flavonoids represent the highest percentage of identified compounds, with abundance of tri- and tetra-glycosilated flavonoids, including acylated ones, especially in L. cicera. Generally, L. digitatus exhibited stronger antioxidant and enzyme inhibitory activities in correlation with its higher level of phenolics. The high number of phenolic compounds and the results of the antioxidant and enzyme assays suggest that these plants may be further used as sources of bioactive compounds, and for the preparation of new nutraceuticals. (C) 2017 Elsevier Ltd. All rights reserved.
Exploring the therapeutic potential and phenolic composition of two Turkish ethnomedicinal plants - Ajuga orientalis L. and Arnebia densiflora (Nordm.), Cheek for updates Ledeb.
(ELSEVIER SCIENCE BV, 2018) Zengin, Gökhan; Ceylan, Ramazan; Katanic, Jelena; Aktümsek, Abdurrahman; Matic, Sanja; Boroja, Tatjana; Stanic, Snezana
Ajuga orientalis L. and Arnebia densiflora (Nordm.) Ledeb. are plants traditionally used in Turkish ethnomedicine to manage common ailments. Nonetheless, there is currently a dearth of investigations geared towards highlighting the inhibitory properties of A. orientalis and A. densiliora against key carbohydrate hydrolyzing enzymes (alpha-amylase and alpha-glucosidase), tyrosinase, and cholinesterases (acetylcholinesterase and butyrylcholinesterase) involved in diabetes, post inflammatory hyperpigmentation, and neurodegenerative diseases, respectively. We aimed to shed light on the antioxidant potential and various enzymatic inhibitory properties of tested methanolic extracts of these plants. In vitro antioxidant activity, DNA protective effects, antimicrobial activity (against 8 bacteria, 10 fungal strains and 1 yeast), as well as phenolic compounds determination and HPLC (high performance liquid chromatography) analysis were performed. 17 phenolic components were quantified in tested extracts. Major phenolic compounds present in A. orientalis extract were chlorogenic acid, benzoic acid, and luteolin. A. densiflora extract revealed the presence of gallic acid, rutin, rosmarinic acid, quercetin, luteolin, and apigenin. A. densiflora showed a stronger antioxidant capacity, higher total phenolic (33.72 mg GAE/g), and total flavonoid contents (55.93 mgRE/g), as well as protection of DNA against hydroxyl radical compared to A. orientalis extract. The tested extracts showed similar antimicrobial activity with low effect against the bacterial and fungal strains. The tested extracts showed a slightly higher potential to inhibit tyrosinase activity, while inhibition of the activity of the other examined enzymes by the extracts was weak. The observed biological activities and presence of bioactive phytochemicals can open new perspectives to develop novel dietetic supple. ments.
Functional constituents of six wild edible Silene species: A focus on their phytochemical profiles and bioactive properties
(ELSEVIER SCIENCE BV, 2018) Zengin, Gökhan; Mahomoodally, Mohamad Fawzi; Aktümsek, Abdurrahman; Ceylan, Ramazan; Uysal, Şengül; Mocan, Andrei; Yılmaz, Mustafa Abdullah
Six wild species (S. alba, S. conoidea, S. dichotoma, S. italica, S. supina, and S. vulgaris) from the Silene genera were tested for potential anti-enzymatic (acetyl cholinesterase (AChE), butyryl cholinesterase (BChE), tyrosinase, alpha-amylase, and alpha-glucosidase), antimicrobial (16 microbial strains), and antioxidant activity. An ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry method was used for phytochemical determination. Quinic acid, malic acid, protocatechuic acid, p-coumaric acid, and hesperidin were common in the six Silene species. All extracts showed higher antibacterial effects compared to streptomycin and ampicillin (except S. dichotoma). Antifungal agents, bifonazole (MIC 0.10-0.20 mg/mL and MFC 0.20-0.30 mg/mL) and ketoconazole (MIC 0.15-2.30 mg/mL and MFC 0.20-3.50 mg/mL) showed lower activity than the investigated Silene species extracts. S. alba inhibited AChE (2.00 mg GALAE/g extract) and BChE (1.0 mg GALAE/g extract). The results showed metal chelating potential ranging from 12 to 19 mg EDTAE/g extract, with S. conoidea being the most active, and S. supina the least. S. dichotoma showed the highest reducing potential against both cupric (154 mg TE/g extract for CUPRAC) and ferric (102 mg TE/g extract for FRAP) ions. Overall, Silene species could be considered as emerging interesting functional foods and sources of nutraceuticals with applications in the management of different diseases.
GC-MS analysis and in vitro antioxidant and enzyme inhibitory activities of essential oil from aerial parts of endemic Thymus spathulifolius Hausskn. et Velen
(TAYLOR & FRANCIS LTD, 2016) Ceylan, Ramazan; Zengin, Gökhan; Uysal, Şengül; İlhan, Veli; Aktümsek, Abdurrahman; Kandemir, Ali; Anwar, Farooq
We investigated the antioxidant and enzyme inhibitory activities and chemical composition of the hydro-distilled essential oil (0.35% yield) from aerial parts of Thymus spathulifolius. Antioxidant capacity of the oil was assessed by different methods including free radical scavenging (DPPH and ABTS), reducing power (FRAP and CUPRAC) and phosphomolybdenum assay. Inhibitory activities were analyzed against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), alpha-amylase, alpha-glucosidase, and tyrosinase. Twenty-one constituents were identified representing 97.2% of the total oil with thymol (50.5%), borneol (16.7%) and carvacrol (7.7%) as the major components. The essential oil exhibited good antioxidant activity with IC50 values of 3.82 and 0.22 mg/mL determined by free radical scavenging DPPH and ABTS, respectively. EC50 values of FRAP and CUPRAC were found to be 0.12 and 0.34 mg/mL, respectively. The results of the present study support the uses of T. spathulifolius essential oil as a source of natural antioxidants and bioactivities for functional foods and phytomedicines.

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