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Öğe Determinations of mRNA's For TGF beta(2,3) in Mouse Testes and Ovaries After a Five days Injection With Glycine or a Serine and Threonine Mixture(MEDWELL ONLINE, 2008) Ciftci, H. B.This study is presented to test the effect of injecting a mixture of Serine/Threonine or Glycine amino acids to determine and measure the changes in mRNAs expression for TGF beta(2.3) within the mouse testes and the ovaries. Male (n = 30) and female (n = 30) mice (CD-1 strain) were injected, once a day for 5 days, with 0.2 mL Saline (control), 0.2 mL Saline with a mixture of Serine/Threonine (test) containing 0.26 mu g L Threonine, 0.13 mu g L Serine or 38 ng Glycine (Test). Total RNA was extracted and transferred on to a nylon membrane then probed with P-32-lebelled cDNA probes. The expression of mRNA for TGF beta(2) increased in male mice while there were no differences in female mice. This study shows that TGF beta(2.3) are expressed both in the ovary and testis and their expressions were increased by the injection of Serine/Threonine or glycine.Öğe Effect of estradiol-17 beta on follicle-stimulating hormone secretion and egg-laying performance of Japanese quail(CAMBRIDGE UNIV PRESS, 2012) Ciftci, H. B.The aim of this study was to measure the effect of estradiol-17 beta (E-2) injection on follicle-stimulating hormone (FSH) secretion and egg-laying performance of Japanese quail. Female Japanese quail were housed in cages and fed ad libitum. After a 7-day adaptation period, the birds were randomly assigned to three groups, that is, one control group and two test groups. The birds were weighed, before every injection. The control group was subcutaneously injected with 0.2 ml sesame oil-ethanol mixture, whereas test groups were injected, twice in a week, with 0.2 ml sesame oil-ethanol mixture containing 0.1 or 0.2 mg E-2 along the study. One day after the first injection, egg number, egg weight, eggshell strength and food conception were daily recorded. On the last day of the experiment, the birds were injected and 3 h later seven birds from each group were randomly selected for bleeding. Blood samples (2 ml/bird) were collected from the jugular vein for the measurements of serum concentrations of E-2, FSH, calcium (Ca) and phosphorus (P). E-2 injection did not cause any significant changes in serum FSH concentrations, daily egg laid/bird, food conception/bird, serum concentrations of the Ca and the P. Egg weight was significantly increased in the 0.1 mg E-2-injected group as compared with the control and 0.2 mg E-2-injected groups. Eggshell strength in the 0.2 mg E-2-injected group was significantly high as compared with the control, whereas the difference between the 0.1 mg E-2- and 0.2 mg E-2- injected groups was not statistically important. These results show that serum FSH concentration was not increased even when slightly suppressed by subcutaneous injection of 0.1 or 0.2 mg E-2. Different doses of E-2 have different functions. The increase in BWs in the 0.1 mg E-2-injected group was a result of the dose effect, which probably increased growth hormone secretion from the pituitary or IGF-1 synthesis from the liver or both. The dose, 0.2 mg E-2, was ineffective in increasing the BW, but it significantly increased eggshell strength probably via the increase in Ca and P utilizations.Öğe EFFECT OF SUPPLEMENTATION OF OVINE OVIDUCTAL CELLS WITH LH AND OESTRADIOL-17 beta ON EMBRYO DEVELOPMENT(INDIAN VETERINARY JOURNAL, 2008) Ciftci, H. B.; Zulkadir, U.; Boztepe, S.; Ozturk, A.; Dag, B.; Yetisir, R.In sheep, towards the end of follicular phase of the oestrus cycle, plasma concentration of E 2 increases and this increase is followed by an increase in LH secretion. The secretion of E 2 and LH can affect the secretary function of the oviductal cells and hence facilitate embryonic development in vitro. Therefore, the aim of this study was to measure the effect of supplementing the oviductal cells with Luteinizing Hormone,(LH) and oestradiol 17 beta (E(2)) on embryo development.Öğe Poultry semen cryopreservation technologies(CAMBRIDGE UNIV PRESS, 2018) Ciftci, H. B.; Aygun, A.Several techniques have been developed for the preservation and improvement of genetic resources to maintain genetic diversity. Among those techniques, semen cryopreservation is thought to be the best and successfully applied by dairy and beef industries, but so far, it has not been established in the poultry industry. This is because poultry sperm cells have a unique shape and membrane fluidity, differing from those of mammalian sperm. Also, poultry sperm membranes contain higher quantities of polyunsaturated fatty acids than mammalian sperm, and hence may require more antioxidant protection. Due to the peculiarity of poultry sperm cells, commonly used cryoprotectants for cryopreservation have a contraceptive or toxic effect. This renders the fertility of frozen poultry sperm to become highly variable and not reliable enough for use in commercial production or preservation of genetic resources. The average fertility of frozen/thawed poultry sperm ranges between 2-80%. Therefore, this paper reviews the possible reasons for the lower success of poultry sperm cryopreservation.Öğe Structures, Functions and Expressions of GnRH and GnRH Receptor in Peripheral Reproductive Organs and Their Regulation by Estradiol-17 beta(ISLAMIC AZAD UNIV, RASHT, 2015) Ciftci, H. B.Studies have shown that estradiol-17 beta (E-2) regulates gonadotropin-releasing hormone (GnRH) and GnRH receptor expression in hypothalamus and pituitary. Several studies have shown that GnRH and its receptor are also expressed in peripheral reproductive organs and little is known about their regulations. In this study, GnRH and GnRH receptor structures, functions, their peripheral expressions and regulations by E-2 were reviewed. Several in vivo and in vitro conducted studies indicate that E-2 decreases the expression of GnRH mRNA and regulates GnRH receptor expression in a time dependent manner. Nevertheless, the exact mechanism has not been clearly explained yet.
