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Öğe ANTIOXIDANT ACTIVITY AND FATTY ACID COMPOSITION OF GINKGO BILOBA FROM TURKEY(WILEY-BLACKWELL, 2011) Maltas, Esra; Vural, Hasibe Cingilli; Yildiz, SalihIn this study, we investigated the antioxidant capacity and total phenolic content of the extracts Ginkgo biloba from Turkey. The antioxidant activity of the methanolic and acetone extracts from G. biloba leaves was measured by various assays, including ferric reducing antioxidant power assay, cupric reducing antioxidant capacity assay and metal chelating capacity. Total phenolic content of the extracts was measured as gallic acid equivalents (GAE) by Folin-Ciocalteu reagent. The methanolic extract showed higher antioxidant activity related to high phenolic content with 76.0 +/- 5.2 mg GAE/g dry weight. Fatty acid compositions of the methanolic and acetone extracts of G. biloba were analyzed. Data suggested that G. biloba grown in Turkey may be an important source of natural antioxidant.Öğe Binding interactions of niclosamide with serum proteins(FOOD & DRUG ADMINSTRATION, 2014) Maltas, EsraA study of the binding of niclosamide (NC) to serum proteins such as human serum albumin, hemoglobin, and globulin was carried out using fluorescence and UV-visible spectroscopy. Interactions between NC and these proteins were estimated by Stern-Volmer and van't Hoff equations. The binding constants and the thermodynamic parameters, Delta H, Delta S, and Delta G at different temperatures were also determined by using these equations. Data showed that NC may exhibit a static quenching mechanism with all proteins. The thermodynamic parameters were calculated. Data showed that van der Waals interactions and hydrogen bonds are the main forces for human serum albumin and hemoglobin. Globulin, however, bound to NC via hydrophobic interaction. The spectral changes of synchronous fluorescence suggested that both the microenvironment of NC and the conformation of the proteins changed in relation to their concentrations during NC's binding. Copyright (C) 2014, Food and Drug Administration, Taiwan. Published by Elsevier Taiwan LLC. All rights reserved.Öğe Binding of actin to thioglycolic acid modified superparamagnetic nanoparticles for antibody conjugation(ELSEVIER, 2015) Maltas, Esra; Ertekin, BetulThioglycolic acid modified superparamagnetic iron oxide nanoparticles (TG-APTS-SPION) were synthesized by using (3-aminopropyl) triethoxysilane (APTS) and thioglycolic acid (TG). Actin was immobilized on the nanoparticle surfaces. Binding amount of the actin (Act) on TG-APTS-SPIONs was determined by using a calibration curve equation that was drawn using fluorescence spectra at 280 and 342 nm of excitation and emission wavelengths. Anti-Actin (anti-Act) was interacted with the actin immobilized TG-APTS-SPIONs as primary antibody. Horse radish peroxidase (HRP) was also interacted with antibody conjugated nanoparticles as secondary antibody. The binding capacity of primary and secondary antibodies was also estimated by fluorescence spectroscopy. Scanning electron microscopy (SEM), Infrared spectroscopy (FTIR) and energy dispersive X-ray (EDX) analysis were also clarified binding of the protein and antibodies to the nanoparticles' surfaces. Western blot analysis was also done for actin conjuction with anti Act antibody to confirm binding of the antibody to the protein. (C) 2014 Elsevier B.V. All rights reserved.Öğe Biochemical and molecular analysis of soybean seed from Turkey(ACADEMIC JOURNALS, 2011) Maltas, Esra; Dageri, Nazan; Vural, Hasibe Cingilli; Yildiz, SalihWe aimed molecular analysis of soybean by lecithin specific primer pairs LE5/LE6. Genomic DNA was extracted from soybean by CTAB method and EZ1 nucleic acid isolation system. A sensitive qualitative detection method for soybean, using the polymerase chain reaction was developed with E5/LE6 primers, produced a 195 bp product. However, the antioxidant activity of methanolic extract of soybean from Turkey by using DPPH and ABTS radical scavenging assays, showed higher antioxidant activity with 53.19 +/- 0.87% and 45.10 +/- 0.32%, respectively. Fatty acid compositions and several phenolic acids and flavonoids of soybean extract were analysed by gas and high performenace chromatography. Data showed that, the main compounds of the extract were eriodictyol, naringenin and linoleic acid.Öğe Combined voltammetric and spectroscopic investigation of binding interaction between nifedipine and human serum albumin on polyelectrolyte modified ITO electrode(PERGAMON-ELSEVIER SCIENCE LTD, 2013) Ozmen, Mustafa; Maltas, Esra; Patir, Imren Hatay; Bayrakci, MevlutThe binding interaction between the drug molecule, nifedipine (Nf), and the human serum albumin (HSA) on polyelectrolyte modified indium tin oxide (ITO) electrode has been investigated by the combination of electrochemical and fluorescence spectroscopy techniques. Surface modification has also been characterized by scanning electron microscopy (SEM) and Contact Angle (CA) measurements in each step. The cyclic voltammetry, electrochemical impedance parameters (peak potential difference (Delta E-p)), peak current difference (Delta I-p) and charge-transfer resistance (R-ct) indicate that nifedipine strongly interacted with human serum albumin molecule on the polyelectrolyte modified ITO electrode surface. Stern-Volmer quenching constant K-a is inversely correlated with the temperature, which indicates that the probable quenching mechanism of the nifedipine-human serum albumin binding reaction is initiated by complex formation. The results obtained from these techniques showed that Nf could bind to HSA. The binding constant (K-b) and the number of binding sites (n) of the drug with HSA at different temperatures were determined. At 298 K, K-b was found as 4.04 x 10(-3) and n was 1.08 for Nf-HSA. According to the van't Hoff equation, the thermodynamic parameters, Delta G, Delta H and Delta S, were obtained, showing the involvement of hydrophobic and electrostatic force in this interaction. (C) 2013 Elsevier Ltd. All rights reserved.Öğe Design of a Probe Based on Poly(glycidyl methacrylate-co-vinylferrocene)-Coated Pt Electrode for Electrochemical Detection of PTEN Gene in PCR Amplified Samples from Prostate Tissues(WILEY, 2014) Bas, Salih Zeki; Maltas, Esra; Sennik, Busra; Yilmaz, Faruk; Vural, Hasibe CingilliIn this study, a new probe based on immobilization of amino linked oligonucleotide (NH2-linked DNA) on poly(glycidyl methacrylate-co-vinylferrocene)-coated Pt electrode was fabricated for the electrochemical detection of PTEN gene from human prostate tissues. The experimental parameters such as DNA immobilization time, DNA concentration, and target concentration were optimized. The selectivity of the NH2-linked DNA probe was assessed with mismatch (MM) and noncomplementary (NC) sequences. The applicability of the NH2-linked DNA probe to the PCR amplified samples correspond to PTEN gene from prostate tissues was evaluated. The immobilization of DNA on the copolymer was confirmed by FTIR, AFM, CV and DPV analysis. The PCR products were also identified by using agarose gel electrophoresis. The prepared probe indicated a linear range (10-100 g mL(-1)) with a detection limit (4.7 g mL(-1)) and a good selectivity of the NH2-linked DNA probe toward target DNA sequence. (c) 2014 Wiley Periodicals, Inc.Öğe Design of an electrochemical biosensing system for xanthine detection and a study on binding interaction of ketoconazole with xanthine oxidase(ELSEVIER SCIENCE BV, 2014) Bas, Salih Zeki; Maltas, Esra; Sennik, Busra; Yilmaz, FarukXanthine oxidase (XO) was successfully immobilized by covalent attachment on poly(glycidyl methacrylate-co-vinylferrocene) (P(GMA-co-VFc)), a redox copolymer containing pendant epoxy and ferrocene moieties, for the evaluation of both the biosensing properties and the effect of the interaction of ketoconazole (Ktc) with the immobilized XO. The binding interaction between Ktc, a drug used to treat fungal infections, and the immobilized XO on P(GMA-co-VFc) was also studied by fluorescence spectroscopy technique. The binding capacity of the drug was determined using a calibration curve equation that was drawn at excitation wavelength of 300 nm using fluorescence spectroscopy. The interaction ability was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and native-PAGE analysis. The enzyme electrode exhibited a linear range from 2.7 x 10(-3) to 0.55 mM with a sensitivity of 19.42 mu A mM(-1) cm(-2) and a detection limit of 8 x 10(-4) mM for the detection of xanthine. The activation energy (E-a) and the apparent Michaelis-Menten constant (K-mapp) values were found to be 12.30 kJ mol(-1) and 0.38 mM, respectively. (C) 2013 Elsevier B.V. All rights reserved.Öğe Development of New Fluorescent Dyes by Using Amine Modified Nanoparticles for Immunostaining(TAYLOR & FRANCIS INC, 2016) Maltas, Esra; Ozmen, Mustafa; Ucan, H. IsmetFluorescent labeling of DNA was studied by using two newly synthesized ligands, which are 4-(2-hydroxybenzylidinamino) benzoic acid (PHBA) and 4-(2-hydroxybenzylidinamino) phthalic acid (PHPA). Their binding capacity to calf-thymus DNA was determined on functionalized superparamagnetic iron oxide nanoparticles (SPIONs). 23.1 mu g of DNA was immobilized on 25mg of nanoparticles. Binding capacity of fluorescent ligands to DNA-APTS-SPIONs was also found as 2.36 mu M for PHBA and 1.97 for PHPA at 25mg of the nanoparticles. Binding of the DNA to the ligands were examined by scanning electron microscopy, transmission electron microscopy, infrared spectroscopy, and confocal microscopy.Öğe DISTRIBUTION OF SECONDARY METABOLITES IN BRASSICA NAPUS GENOTYPES(WILEY-BLACKWELL, 2011) Maltas, Esra; Yildiz, SalihSecondary metabolites (flavonoids and phenolics) in the methanolic extracts of Brassica napus genotypes, spring and winter canola were evaluated. Antioxidant activity of the extracts was evaluated by beta-carotene linoleic acid model system, scavenging ability of hydrogen peroxide (H(2)O(2)) assay and cupric reducing antioxidant capacity (CUPRAC) assay. Also, total flavonoid contents of the extracts were determined by aluminum chelating method. Results showed that naringin and eriodictyol were found to be in both species. Data showed that winter canola exihibited high antioxidant capacity with 60.6 +/- 0.4% in beta-carotene linoleic acid model system and also CUPRAC value (4.18 +/- 0.11 mM TEAC/g) and scavenging ability of H(2)O(2) (57.12 +/- 1.13%) was higher than spring canola. However, total flavonoid content of spring canola was higher than that of winter canola. Both canola species may be of importance in variety improvement, nutraceuticals, bio-pharmaceuticals and food additives as natural antioxidants.Öğe Extraction of genomic DNA from polysaccharide- and phenolics-rich Ginkgo biloba(ACADEMIC JOURNALS, 2011) Maltas, Esra; Vural, Hasibe Cingilli; Yildiz, SalihOne prerequisite to reliable molecular biology work is that the genomic DNA of a sample should be of good quality. The isolation of intact, high-molecular-mass genomic DNA is essential for many molecular biology applications including long Polymerase Chain Reaction (PCR), endonuclease restriction digestion, Southern blot analysis, and genomic library construction. Many protocols are available for the extraction of DNA from plant material. DNA extraction of Ginkgo biloba is quite difficult to work on because of the high phenolic and polysaccharide content of its leaves. This study aimed to determine which protocol to use and which part of Ginkgo tree is most appropriate to extract good-quality genomic DNA. For this purpose, cetyltrimethylammonium bromide protocol and protocol of commercially available kit by EZ1 Nucleic acid isolation system have been optimized for extraction of genomic DNA from G. biloba leaves. Efficient yields of high-quality amplifiable DNA was produced rapidly with kit by EZ1 Nucleic acid isolation method. The purified DNA which has excellent spectral quality was efficiently amplified by 5 arbitrary primers (OPA11-15), and was suitable for long-fragment PCR amplification.Öğe Fabrication of protein scaffold by electrospin coating for artificial tissue(ELSEVIER SCIENCE BV, 2016) Ozcan, Fatih; Ertul, Seref; Maltas, EsraProtein nanofiber scaffolds were produced by electrospin coating for biomedical applications. Therefore, protein such as bovine serum albumin, gobulin and hemoglobin were used as scaffold for tissue engineering. The nanofiber diameters were found to be 75 nm of globulin, 117 nm for hemoglobin and 220 nm for BSA. The protein nanofibers were characterized by using Fourier transform infrared spectroscopy, Scanning electron microscopy, Transmission electron microscopy and Atomic force microscopy. (C) 2016 Elsevier B.V. All rights reserved.Öğe Fluorescent labelling of DNA on superparamagnetic nanoparticles by a perylene bisimide derivative for cell imaging(ELSEVIER SCIENCE BV, 2015) Maltas, Esra; Malkondu, Sait; Uyar, Pembegul; Ozmen, MustafaN,N'-Bis[tris-(2-aminoethyl) amine]-3,4,9,10-perylenetetracarboxylic diimide (PBI-TRIS), nonfluorescent dye was used to fluorescent labelling of DNA. For this aim, (3-aminopropyl) triethoxysilane (APTS) modified superparamagnetic iron oxide nanopartides (SPIONs) were synthesized to provide a suitable surface for binding of DNA. Amine functionalized nanoparticles showed a high immobilization capacity (82.70%) at 25 mg of nanoparticle concentration for Calf thymus DNA. Binding capacity of PBI-TRIS to DNA-SPION was also found as 1.93 mu M on 25 mg of nanoparticles by using UV-vis spectroscopy. Binding of PBI-TRIS to DNA onto nanoparticles was also characterized by scanning electron microscopy and infrared spectroscopy. The confocal images of PBI-TRIS labelled DNA-SPION and breast cells were taken at 488 and 561.7 nm of excitation wavelengths. Cell image was also compared with a commercial dye, DAPI at 403.7 nm of excitation wavelength. Results showed that PBI-TRIS can be used for cell staining. (C) 2014 Elsevier B.V. All rights reserved.Öğe Interaction Between Ketoconazole and Human Serum Albumin on Epoxy Modified Magnetic Nanoparticles for Drug Delivery(AMER SCIENTIFIC PUBLISHERS, 2013) Maltas, Esra; Ozmen, Mustafa; Yildirimer, Burak; Kucukkolbasi, Semahat; Yildiz, SalihSuperparamagnetic iron oxide nanoparticles (SPIONs) were modified with [3-(2,3-epoxypropoxy)propyl] trimethoxy silane, which resulted in formation of epoxy groups on the particles surface. The epoxy functionalized SPIONs can bind to human serum albumin (HSA). The binding amount of HSA to epoxy modified SPIONs was found to be as 32.7 mu M for 1 mg epoxy modified SPIONs at 280 and 342 nm of excitation and emission wavelengths by using fluorescence spectroscopy. Interaction of ketoconazole with HSA immobilized epoxy functionalized SPIONs was studied at 300 and 390 nm of excitation and emission wavelengths, respectively. Binding mechanism of ketoconazole and HSA was identified by Stern-Volmer equation. Thermodynamic parameters (Delta H, Delta S and Delta G) were also estimated for the interaction. Therefore, the nature of the binding forces was found to be as hydrophobic interaction. Protein and drug attachments were also examined by Infrared spectroscopy (IR) and Scanning Electron Microscopy (SEM). This study show that prepared albumin-based magnetic nanoparticles carrier systems represents an attractive strategy for drug delivery.Öğe Interaction of donepezil with human serum albumin on amine-modified magnetic nanoparticles(ELSEVIER SCIENCE BV, 2014) Buzoglu, Leman; Maltas, Esra; Ozmen, Mustafa; Yildiz, SalihThe interaction between the drug donepezil and human serum albumin (HSA) was examined on the surface of amine modified superparamagnetic iron oxide nanoparticles (SPIONs), which were synthesized by the coprecipitation of ferrous and ferric salts with NH4OH and then modified with [3-Aminopropyl] triethoxysilane (APTES) to obtain functional amine groups on the nanoparticles' surface. Albumin's binding capacity to APTES-modified SPIONs was estimated by fluorescence spectroscopy. After HSA was bound to the APTES modified SPIONs, donepezil was interacted with the HSA-SPIONs. The binding capacity of the drug was determined using a calibration curve equation that was drawn using fluorescence spectroscopy at 325 and 387 nm, the excitation and emission wavelengths. Thermodynamic parameters were estimated for the interaction of HSA and donepezil on amine-modified SPIONs. Binding was carried out spontaneously via electrostatic interaction. Binding was also examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SOS-PAGE), scanning electron microscopy (SEM) and zeta-potential measurements. (C) 2013 Elsevier B.V. All rights reserved,Öğe Interaction of L-myc oncogene in breast cancer with irinotecan onto functionalized magnetic nanoparticles(ELSEVIER SCIENCE BV, 2013) Maltas, Esra; Ozmen, Mustafa; Vural, Hasibe CingilliThe effect of different functional groups on binding capacity of DNA was studied with bare and modified superparamagnetic iron oxide nanoparticles (SPIONs). For this purpose, modifications were performed with [3(2,3-epoxypropoxy)propyl] trimethoxy silane (GPTS) and (3-aminopropyl) triethoxysilane (APTS) by silanization reaction and also subsequent reaction with glutaraldehyde (GA) to obtain functional groups on nanoparticles surface. L-myc gene from breast tissue with cancer was amplified by using polymerase chain reaction (PCR). APTS functionalized nanoparticles showed the highest binding capacity (82.70%) when compared with bare, GPTS, APTS and GA functionalized SPIONs. DNA immobilized magnetic nanoparticles were also examined by Scanning Electron Microscopy. Binding capacity of irinotecan to DNA onto SPIONs was found as 2.81 x 10(-5) mg/mL for 20 mg of APTS modified nanoparticles by using fluorescence spectroscopy. (C) 2013 Elsevier B.V. All rights reserved.Öğe Magnetic nanoparticles-serum proteins bioconjugates for binding of irinotecan(ELSEVIER, 2015) Tamyurek, Ecem; Maltas, Esra; Bas, Salih Zeki; Ozmen, Mustafa; Yildiz, SalihThe binding of irinotecan to serum proteins (hemoglobin, globulin and human serum albumin) was studied on the surface of epoxide modified superparamagnetic iron oxide nanoparticles (GPT'S-SPIONs), which were synthesized by the coprecipitation of ferrous and ferric salts with NH4OH and then modified with [3-(2,3-epoxypropoxy)propyl] trimethoxy silane (GPTS) to obtain functional epoxide groups on the SPIONs' surface. Results were compared to find an alternative as drug carries system. Data showed that binding amount of human serum albumin (HSA), globulin (Gib) and hemoglobin (Hb) found to be as 44, 21.2 and 32.6 mu g per 20 mg of GPTS modified SPIONs, respectively. The thermal behavior of the serum protein-Ir interaction on GPTS-SPIONs was also studied by using thermo gravimetric analysis (TGA) technique and then the kinetic parameters for the thermal decomposition were determined using Horowitz-Metzger method. (C) 2014 Elsevier B.V. All rights reserved.Öğe A new BODIPY/nanoparticle/Ni affinity system for binding of cytochrome c(ELSEVIER SCIENCE BV, 2015) Maltas, Esra; Kursunlu, Ahmed Nuri; Arslan, Gulsin; Ozmen, MustafaIn this study, 3,5-{Bis[4,4-difluoro, 8-(2,6-diethyl, 1,3,5,7-tetramethy1-4-bora-3a,4a-diaza-sindacene)]}benzoylchloride (BODIPY) was synthesized for the improving of a new immobilized metal affinity supporting material. Firstly, the synthesized BODIPY was immobilized on iron oxide superparamagnetic nanoparticles (SPIONs) and then, Ni(II) ions were chelated with the active terminals of BODIPY on nanoparticles surfaces to prepare an immobilized metal affinity (IMA) adsorbent for protein adsorption. The amount of BODIPY coated on SPIONs was about 29.7 mu M at 10 mg nanoparticles. 738 mu mol of Ni(II) ions were loaded to 10 mg of the SPIONs/BODIPY. The binding amount of cytochrome c was found to be 170 mu g to the SPIONs/BODIPY/Ni at pH 7.4. The binding amount of the molecules on SPIONs was analyzed by using UV-vis, fluorescence and atomic absorption spectroscopy. The characterization of the prepared surfaces was performed by FT-IR, SEM and TEM. (C) 2015 Elsevier B.V. All rights reserved.Öğe Novel humic acid-bonded magnetite nanoparticles for protein immobilization(ELSEVIER SCIENCE BV, 2014) Bayrakci, Mevlut; Gezici, Orhan; Bas, San Zeki; Ozmen, Mustafa; Maltas, EsraThe present paper is the first report that introduces (i) a useful methodology for chemical immobilization of humic acid (HA) to aminopropyltriethoxysilane-functionalized magnetite iron oxide nanoparticles (APS-MNPs) and (ii) human serum albumin (HSA) binding to the obtained material (HA-APS-MNPs). The newly prepared magnetite nanoparticle was characterized by using Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and elemental analysis. Results indicated that surface modification of the bare magnetite nanoparticles (MNPs) with aminopropyltriethoxysilane (APS) and HA was successfully performed. The protein binding studies that were evaluated in batch mode exhibited that HA-APS-MNPs could be efficiently used as a substrate for the binding of HSA from aqueous solutions. Usually, recovery values higher than 90% were found to be feasible by HA-APS-MNPs, while that value was around 2% and 70% in the cases of MNPs and APS-MNPs, respectively. Hence, the capacity of MNPs was found to be significantly improved by immobilization of HA. Furthermore, thermal degradation of HA-APS-MNPs and HSA bonded HA-APS-MNPs was evaluated in terms of the Horowitz-Metzger equation in order to determine kinetic parameters for thermal decomposition. Activation energies calculated for HA-APS-MNPs (20.74 kJ mol(-1)) and HSA bonded HA-APS-MNPs (33.42 kJ mol(-1)) implied chemical immobilization of HA to APS-MNPs, and tight interactions between HA and HA-APS-MNPs. (C) 2014 Elsevier B.V. All rights reserved.Öğe SCREENING OF PHYTOCHEMICALS AND ANTIOXIDANT ACTIVITY OF ARUM DIOSCORIDIS SEEDS(WILEY, 2012) Uguzlar, Huseyin; Maltas, Esra; Yildiz, SalihThe aim of this paper was to evaluate the antioxidant properties of the different extracts from Arum dioscoridis seeds (methanolic, acetone and hexane extracts) and to correlate their antioxidant potential to the composition of phenolic compounds. The scavenging ability of free radicals was measured using beta-carotenelinoleic acid model system and 2,2-diphenyl-1-picrylhydrazyl (DPPH.) analysis and the IC50 values of the extracts were also determined. The methanolic extract of A. dioscoridis seeds showed greater antioxidant activity than acetone and hexane extracts by beta-carotenelinoleic acid model system and DPPH. analysis, respectively. Qualitative and quantitative analyses of major phenolic compounds were also performed. The main antioxidant compound from the methanol extract was found to be vitexin. Other identified phenolics in all extracts that are likely to contribute to the antioxidant potential are: ferulic acid, naringin, eriodictyol and p-coumaric acid. PRACTICAL APPLICATIONS Antioxidant properties of the different extracts from Arum dioscoridis seeds (methanolic, acetone and hexane extracts) were evaluated in this study. The scavenging ability of free radicals was measured using the beta-carotenelinoleic acid model system and 2,2-diphenyl-1-picrylhydrazyl (DPPH.) analysis, and the IC50 values of the extracts were also determined. The methanolic extract of A. dioscoridis seeds showed greater antioxidant activity than acetone and hexane extracts, respectively. The main antioxidant compound from the methanol extract was found to be vitexin. Other identified phenolics such as ferulic acid, naringin, eriodictyol and p-coumaric acid in all extracts are likely to contribute to the antioxidant potential.Öğe Spectrofluorometric and thermal gravimetric study on binding interaction of thiabendazole with hemoglobin on epoxy-functionalized magnetic nanoparticles(ELSEVIER, 2015) Maltas, Esra; Ozmen, MustafaThe interaction of thiabendazole (Tbz) with hemoglobin (Hb) on epoxy-functionalized iron oxide nanoparticles was presented in this study. The binding capacity of Tbz was determined by measuring at an excitation wavelength of 299 nm using fluorescence spectroscopy. The thermodynamic parameters of the Hb-Tbz interaction were calculated from Stern-Volmer and van't Hoff equations. The values of enthalpy change, Delta H, and entropy change,.Delta S, were found to be 0.20 kJ mol(-1) and 0.70 J mol(-1) K-1, respectively, which indicates that the hydrophilic interaction plays a main role in the binding process. The interaction ability was confirmed by Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). Also, the thermal behavior of the Hb-Tbz interaction on functionalized iron oxide nanoparticles was studied by using the thermogravimetric analysis (TGA) technique in the temperature range of 25-950 degrees C, and then the kinetic parameters for the thermal decomposition were determined using the Horowitz-Metzger method. (C) 2015 Elsevier B.V. All rights reserved.
