Development of New Fluorescent Dyes by Using Amine Modified Nanoparticles for Immunostaining
Küçük Resim Yok
Tarih
2016
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
TAYLOR & FRANCIS INC
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Fluorescent labeling of DNA was studied by using two newly synthesized ligands, which are 4-(2-hydroxybenzylidinamino) benzoic acid (PHBA) and 4-(2-hydroxybenzylidinamino) phthalic acid (PHPA). Their binding capacity to calf-thymus DNA was determined on functionalized superparamagnetic iron oxide nanoparticles (SPIONs). 23.1 mu g of DNA was immobilized on 25mg of nanoparticles. Binding capacity of fluorescent ligands to DNA-APTS-SPIONs was also found as 2.36 mu M for PHBA and 1.97 for PHPA at 25mg of the nanoparticles. Binding of the DNA to the ligands were examined by scanning electron microscopy, transmission electron microscopy, infrared spectroscopy, and confocal microscopy.
Açıklama
Anahtar Kelimeler
calf-thymus DNA, fluorescence, ligand, magnetic nanoparticle
Kaynak
SYNTHESIS AND REACTIVITY IN INORGANIC METAL-ORGANIC AND NANO-METAL CHEMISTRY
WoS Q Değeri
Q4
Scopus Q Değeri
N/A
Cilt
46
Sayı
12
