Development of New Fluorescent Dyes by Using Amine Modified Nanoparticles for Immunostaining

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dc.contributor.authorMaltas, Esra
dc.contributor.authorOzmen, Mustafa
dc.contributor.authorUcan, H. Ismet
dc.date.accessioned2020-03-26T19:23:35Z
dc.date.available2020-03-26T19:23:35Z
dc.date.issued2016
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractFluorescent labeling of DNA was studied by using two newly synthesized ligands, which are 4-(2-hydroxybenzylidinamino) benzoic acid (PHBA) and 4-(2-hydroxybenzylidinamino) phthalic acid (PHPA). Their binding capacity to calf-thymus DNA was determined on functionalized superparamagnetic iron oxide nanoparticles (SPIONs). 23.1 mu g of DNA was immobilized on 25mg of nanoparticles. Binding capacity of fluorescent ligands to DNA-APTS-SPIONs was also found as 2.36 mu M for PHBA and 1.97 for PHPA at 25mg of the nanoparticles. Binding of the DNA to the ligands were examined by scanning electron microscopy, transmission electron microscopy, infrared spectroscopy, and confocal microscopy.en_US
dc.description.sponsorshipResearch Foundation of Selcuk UniversitySelcuk University [12401018]en_US
dc.description.sponsorshipThis work was supported by the Research Foundation of Selcuk University (12401018).en_US
dc.identifier.doi10.1080/15533174.2015.1137062en_US
dc.identifier.endpage1786en_US
dc.identifier.issn1553-3174en_US
dc.identifier.issn1553-3182en_US
dc.identifier.issue12en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage1779en_US
dc.identifier.urihttps://dx.doi.org/10.1080/15533174.2015.1137062
dc.identifier.urihttps://hdl.handle.net/20.500.12395/33441
dc.identifier.volume46en_US
dc.identifier.wosWOS:000382305800006en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTAYLOR & FRANCIS INCen_US
dc.relation.ispartofSYNTHESIS AND REACTIVITY IN INORGANIC METAL-ORGANIC AND NANO-METAL CHEMISTRYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectcalf-thymus DNAen_US
dc.subjectfluorescenceen_US
dc.subjectliganden_US
dc.subjectmagnetic nanoparticleen_US
dc.titleDevelopment of New Fluorescent Dyes by Using Amine Modified Nanoparticles for Immunostainingen_US
dc.typeArticleen_US

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