DSpace Arşivi :: by Yazar "Uysal, Şengül" değerine göre listeleniyor

Yazar "Uysal, Şengül" seçeneğine göre listele

Listeleniyor 1 - 20 / 46

Akdeniz bölgesinden toplanan bazı meyve yapraklarının antioksidan özelliklerinin araştırılması
(Selçuk Üniversitesi Fen Bilimleri Enstitüsü, 2013-06-06) Uysal, Şengül; Aktümsek, Abdurrahman
Bu çalışmada, Akdeniz bölgesi?nden toplanan dokuz meyve yaprağı örneğinin su ve metanol özütlerinin antioksidan özellikleri tanımlanmıştır. Antioksidan kapasiteleri, DPPH, ABTS, NO ve OH radikallerine karşı radikal giderme aktivitesi ile bunun yanı sıra indirgeme gücü (demir ve bakır) ve fosfomolibdenyum metotları kullanılarak test edilmiştir. Ayrıca ekstraktların, fenolik, flavonoid ve saponin miktarları hesaplanmıştır. Fenolik ve flavonoid miktarları sırası ile Folin ve AlCl3 metotları ile belirlenmiştir. Toplam saponin içeriği spektrofotometrik yöntem (sülfirik asit-vanilin testi) ile tespit edilmiştir.. Toplam fenolik içeriği ekstraktlarda 22.24 ile 274.50 mgGAE/g arasında değişmiştir. Nar yapraklarının su ekstraktı en yüksek fenolik içeriğe sahiptir. En güçlü NO ve OH radikal süpürme aktiviteleri metanolik ekstraktlardan elde edilmiştir. Su ve metanol ekstraktları indirgeme gücü metotlarında hemen hemen aynı aktiviteyi göstermiştir. Metanolik ekstraktlar ß-karoten/linoleik asit testinde linoleik asit oksidasyonunu önemli derecede inhibe etmişlerdir. Çalışmamızın sonuçları, çalışılan yaprak örneklerinden elde edilen su ve metanol özütlerinin sentetik antioksidanlar için potansiyel alternatif olarak düşünülebileceğini ve gıda endüstrisi ile farmasötik uygulamalar için doğal bir antioksidan kaynağı olarak kullanılabileceğini göstermiştir.
Antioxidant properties and enzyme inhibitory effects of extracts from Mandragora autumnalis and its fatty acid composition
(MARMARA UNIV, FAC PHARMACY, 2016) Uysal, Şengül; Zengin, Gökhan; Aktümsek, Abdurrahman
The present research was undertaken to evaluate antioxidant activities and enzyme inhibitory potentials of two extracts from two parts of Mandragora autumnalis. The antioxidant properties were evaluated by using different methods [ABTS (2,2-azino-bis (3-ethylbenzothiazloine-6-sulphonic acid)), DPPH (1,1 diphenyl-2-picrylhydrazyl radical), CUPRAC (cupric ion reducing antioxidant capacity), and FRAP (ferric reducing antioxidant power assays)]. Enzyme inhibitory potentials were tested against cholinesterase (AChE and BChE), tyrosinase, a-amylase and a-glucosidase. For phytochemical composition, fatty acids, total phenolics and flavonoid content were investigated. Generally F-Met exhibited the strongest antioxidant effect with the highest level of phenolics. Total phenolic and flavonoid content were determined as 26.10-46.92 mgGAE/g abd 3.60-26.11 mgRE/g, respectively. a- linolenic acid was found to be major fatty acids in the studied oils (33.81 % for flowers and 39.15 % for leaves). The study suggested that M. autumnalis could be valuable as a source of natural agents in food and pharmaceutical applications.
Biological activities of three extracts from artedia squamata: A study on antioxidant and enzyme inhibitory potential
(Bentham Science Publishers B.V., 2015) Zengin, Gökhan; Ceylan, Ramazan; Uysal, Şengül; Aktümsek, Abdurrahman
The present work focused on antioxidant and enzyme inhibitory potential of different solvent extracts (ethyl acetate, methanol and water) from Artedia squamata. Cholinesterase, tyrosinase, ?-amylase and ?-glucosidase inhibitory activities of the extracts were tested by microtitre plate assays. Antioxidant abilities were tested using free radical scavenging (DPPH and ABTS), reducing power (FRAP and CUPRAC), total antioxidant capacity and ferric ion chelating assays. The total phenolic contents of these extracts ranged from 26.24 to 35.16 mg GAEs/g extract. Methanol and water extracts showed higher phenolic content, DPPH and ABTS scavenging activity and reducing power activities, while ethyl acetate extract had highest chelating and inhibition abilities on AChE, ?-amylase and ?- glucosidase. With regard to tyrosinase inhibitory activity, water extract showed a good inhibitory activity with 11.91 mg KAEs/g. Present study showed that Artedia squamata can be considered as good source of natural agents for food and drug applications. © 2015 Bentham Science Publishers.
Biological, chemical and in silico fingerprints of Dianthus calocephalus Boiss.: A novel source for rutin
(PERGAMON-ELSEVIER SCIENCE LTD, 2018) Uysal, Şengül; Aktümsek, Abdurrahman; Picot-Allain, Carene M. N.; Ünüvar, Hamiyet; Mollica, Adriano; Georgiev, Milen I.; Zengin, Gökhan
Extracts (methanol, ethyl acetate, and water) from Dianthus calocephalus Boiss. prepared by different extraction techniques (maceration, Soxhlet, and ultrasonication) were studied for possible inhibitory action against key enzymes (alpha-amylase, alpha-glucosidase, acetyl cholinesterase, butyryl cholinesterase, and tyrosinase). Antioxidant potential was established using a battery of assays and phenolic compounds profiled by RP-HPLC. Binding pose of tyrosinase with rutin was studied by means of molecular docking. Methanol extracts showed the highest phenolic (39.35-40.25 mgGAE/g) content and rich in rutin (61.38-72.07 mg/g extract). Ethyl acetate extracts of D. calocephalus were potent inhibitors of acetyl (1.45-1.48 mgGALAE/g) and butyryl (2.44-2.74 mgGALAE/g) cholinesterases. Docking studies showed that rutin interacts with the side chains of the key amino acid residues and to the copper atom found at the active site of tyrosinase. Methanol extracts showed highest antioxidant capacity. D. calocephalus showed interesting biological properties that could be further studied to manage diabetes, neurodegenerative diseases, Alzheimer's disease, and hyperpigmentation.
Chemical and biological approaches on nine fruit tree leaves collected from the Mediterranean region of Turkey
(ELSEVIER SCIENCE BV, 2016) Uysal, Şengül; Zengin, Gökhan; Aktümsek, Abdurrahman; Karataş, Şükrü
The antioxidant and enzyme inhibitory activities of two extracts [methanolic and water] of nine different fruit tree leaves were assessed. The antioxidant activity of leaves was determined by ABTS (2,2-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)), DPPH (1,1-diphenyl-2-picrylhydrazyl radical), CUPRAC (cupric ion reducing antioxidant capacity), and FRAP (ferric reducing antioxidant power)assays. The enzyme inhibitory activity was evaluated against acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and tyrosinase. Individual phenolic components were detected using HPLC-DAD (high performance liquid chromatography-diode array detector) technique. Chlorogenic acid was the most abundant compounds in the leaves. Total phenolic and flavonoid contents were determined as 22.24-274.50 milligram gallic acid equivalents (mgGAE/g) and 4.93-46.99 milligram rutin equivalents (mgRE/g), respectively. A higher antioxidant and enzyme inhibitory activity was observed in pomegranate and carob extract as compared to other leaves. This study suggested that the leaves may be beneficial for preparing novel functional food ingredients. (C) 2016 Elsevier Ltd. All rights reserved.
Chemical Profile, Antioxidant Properties and Enzyme Inhibitory Effects of the Root Extracts of Selected Potentilla Species
(ELSEVIER, 2019) Uysal, Şengül; Zengin, Gökhan; Mahomoodally, Mohamad Fawzi; Yılmaz, Mustafa Abdullah; Aktümsek, Abdurrahman
Plants from the genus Potentilla have been traditionally used for therapeutic purposes in Turkey. Methanol root extracts from four selected Potentilla species (P. anatolica, P. argentea, P. recta, and P. reptans) were probed for their chemical composition, antioxidant properties (DPPH, ABTS, FRAP, CUPRAC, phosphomolybdenum, and metal chelating activity), and enzyme (acetylcholinesterase (AChE), butyrylcholinesterase (BChE), alpha-amylase, alpha-glucosidase, and tyrosinase) inhibition effects. In addition, the total phenolic, flavonoid, saponin, and triterpenoid content of the extracts were determined. Ultra-high performance liquid chromatography (UHPLC)-electrospray ionization (ESI)-tandemmass spectrometry (MS/MS) analysis revealed that the main phenolic components in extracts were coumarin, hesperidin, p-coumaric acid, gallic acid, and rutin. P. recta and P. reptans showed the highest total phenolic, saponin, and triterpenoid content. The root extracts showed significant antioxidant properties and depicted significant inhibitory effects against AChE, BChE, alpha-amylase, alpha-glucosidase, and tyrosinase. In conclusion, root extracts of Potentilla may be considered as new natural antioxidants and key enzyme inhibitors that can be exploited as bioproducts for pharmaceutical industries. (c) 2018 SAAB. Published by Elsevier B.V. All rights reserved.
Chemical profile, antioxidant, and enzyme inhibitory properties of two Scutellaria species: S-orientalis L. and S-salviifolia Benth
(WILEY, 2019) Zengin, Gökhan; Llorent-Martinez, Eulogio J.; Molina-Garcia, Lucia; Fernandez-de Cordova, Maria Luisa; Aktümsek, Abdurrahman; Uysal, Şengül; Rengasamy, Kannan R. R.; Aumeeruddy, Muhammad Zakariyyah; Bahadori, Mir Babak; Mahomoodally, Mohamad Fawzi
Objectives This study investigates into the biological effects of solvent extracts (ethyl acetate, methanol, and water) of Scutellaria orientalis L. and Scutellaria salviifolia Benth. based on its enzyme inhibitory activity and antioxidant ability together with the screening of bioactive compounds. Methods Total and individual bioactive components were determined using spectrophotometric and HPLC-ESI-MS methods. Six antioxidant assays were conducted and enzyme inhibition was tested against key enzymes linked to the pathology of common chronic disorders. Key findings Results revealed that the aqueous extracts of both plants exerted better 2,2 '-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid scavenging, reducing power, metal chelating, and alpha-glucosidase inhibitory activities. The methanol extracts showed highest tyrosinase inhibition and antioxidant activity in phosphomolybdenum assay while the less polar ethyl acetate extracts showed better acetylcholinesterase, butyrylcholinesterase, and alpha-amylase inhibition. Phytochemical evaluation by HPLC-ESI-MS revealed the presence of high amounts of flavones. Conclusions Scutellaria orientalis and S. salviifolia are important sources of bioactive agents that warrants further studies.
A Comparative fatty acid compositional analysis of different wild species of mushrooms from Turkey
(UNITED ARAB EMIRATES UNIV, 2015) Zengin, Gökhan; Sarıkürkcü, Cengiz; Aktümsek, Abdurrahman; Uysal, Şengül; Ceylan, Ramazan; Anwar, Farooq; Solak, Mehmet Halil
Mushrooms have been recognized as important food items due to their high nutritional and medicinal value. The present work quantifies and compares the fatty acids composition among eleven mushroom species (Auricularia auricular-judae, Collybia dryophila, Rammulina ve/utipes, Helvella lacunose, Polyporus squamosus, Rhizopogon roseolus, Russula albonigra, Russula delica, Sparassis crispa, collinitus and Volvariella gloiocephala) harvested from Turkey. The lipid contents of the mushrooms varied from 0.13% (Auricularla auricula-judae) to 2.90% (Sparassis crispa). A total of 14 fatty acids were identified and quantified by gas chromatography in the selected mushrooms species with linoleic (13.17 to 79.41%), oleic (0.71 to 55.07%) and palmitic (8.95 to 30.84%) acids as the major components in the tested mushrooms species. The amount of total saturated fatty acids (SFAs), total monounsaturated FAs (MUFAs) and total polyunsaturated FAs (PUFAs) ranged from 13.97 to 59.44%, 0.94 to 55.44% and 13.54 to 79.93%, respectively. Moreover, the mushrooms tested can be explored as a rich dietary source of essential fatty acids (13.34-79.76%) for human nutrition.
A comparative in vitro and in silico study of the biological potential and chemical fingerprints of Dorcycinum pentapyllum subsp haussknechtii using three extraction procedures
(ROYAL SOC CHEMISTRY, 2017) Uysal, Şengül; Aktümsek, Abdurrahman; Picot, Carene M. N.; Sahan, Alime; Mollica, Adriano; Zengin, Gökhan; Mahomoodally, Mohamad Fawzi
We probed into the effect of different extraction procedures (maceration, Soxhlet extraction, and ultrasonication) on the phytochemical profiles and bioactivities of ethyl acetate, methanol, and water extracts of Dorcycinum pentapyllum subsp. haussknechtii. The antioxidant (ABTS and DPPH), reducing power (CUPRAC and FRAP), phosphomolybdenum, metal chelation, and enzyme inhibitory activities (acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase, a-amylase, and a-glucosidase) were determined. The total phenolic and flavonoid contents together with the phenolic compounds were evaluated by RP-HPLC. Molecular modeling calculations were applied to relevant bioactive compounds observed in the extracts and docked to targeted enzymes used in order to elucidate their docking characteristics and possible interactions. The type of extraction technique was found to affect the concentration of phytochemicals (maceration, Soxhlet extraction, and ultrasonication gave 3052, 3616, and 3252 mg g(-1) extract, respectively). The highest values of the total phenolic content and antioxidant activities followed the same order, highlighting a possible relationship between strong antioxidant action and high phytochemical content. Ethyl acetate extracts were potent inhibitors of a-amylase while methanol extracts showed pronounced activity against a-glucosidase. Methanol extracts of D. pentapyllum were potent inhibitors of AChE. BChE was inhibited by water extracts. The best docking pose and DG binding energy calculations revealed that quercetin had a preference for a-amylase and AChE, whereas rutin was capable of docking efficaciously to BChE, a-glucosidase, and tyrosinase. Evidence from the present study advocates the need for exploring different extraction techniques in an endeavour to discover unique bioactive lead compounds that could target specific ailments.
Comparative study of biological activities and multicomponent pattern of two wild Turkish species: Asphodeline anatolica and Potentilla speciosa
(TAYLOR & FRANCIS LTD, 2016) Zengin, Gökhan; Menghini, Luigi; Malatesta, Luciano; De Luca, Elisa; Bellagamba, Giuseppe; Uysal, Şengül; Aktümsek, Abdurrahman
The multicomponent pattern and biological characterization of plant material are essential for pharmaceutical field, in the food supplements quality control procedures and to all plant-based products. These nutrients often show valuable effects related to their consumption due to the occurrence of secondary metabolites that show useful properties on health. In this framework, researches performed on this topic play a central role for human health and drug development process. The aim of this study was to compare phenolics and free anthraquinones multicomponent pattern of two wild Turkish species: Asphodeline anatolica and Potentilla speciosa using validated high-performance liquid chromatography-photogiode array (HPLC-PDA) assays, coupled to biological evaluation. Even if some variances related to biological and enzymatic inhibition activities can be ascribed to other phytochemicals, the reported data support traditional use of Asphodeline anatolica and Potentilla speciosa roots as valuable natural font for the development of novel natural-derived drug formulations and/or food supplements with health and nutritional benefits.
A comparative study of Bulgarian and Turkish Asphodeline lutea root extracts: HPLC-UV profiles, enzyme inhibitory potentials and anti-proliferative activities against MCF-7 and MCF-10A cell lines
(ELSEVIER SCIENCE BV, 2015) Lazarova, Irina; Zengin, Gökhan; Bender, Onur; Zheleva-Dimitrova, Dimitrina; Uysal, Şengül; Ceylan, Ramazan; Gevrenova, Reneta
Asphodeline lutea (L.) Rchb. is a wild plant traditionally used as a food in the Mediterranean region. The alcoholic extracts of A. lutea roots from different origins (Bulgarian and 'Rakish) were investigated for anti-cholinesterase, anti-tyrosinase, anti-amylase, anti-glycosidase and anti-proliferative activity (against MCF-7 and MCF-10A cell lines). A validated HPLC method for the determination of two anthraquinones, two bianthraquinones, two naphthalenes, two flavonoids and one hydroxycinnamic acid was developed due to the presence of these components in the genus Asphodeline. In addition, total anthraquinones content was determined spectrophotometrically. A. lutea from Bulgaria demonstrated higher level of total anthraquinones (0.81 +/-0.05 mg g(-1)). A naphthalene derivative (2-acetyl-1,8-dimethoxy-3-methylnaphthalene) was the major compound in Bulgarian accession (0.96 +/- 0.03 mg g(-1)), while caffeic acid was the main analyte in Turkish sample (0.42 +/- 0.06 mg g(-1)). Turkish extracts were more potent as cholinesterase and tyrosinase inhibitors but less active as antidiabetic agent. The higher anti-proliferative effect was presented by Bulgarian extract with IC50 values 120 +/- 16.8 mg mL(-1) (for MCF-7) and 256 +/- 16.1 mg mL(-1) (for MCF-10A). (C) 2015 Elsevier Ltd. All rights reserved.
Composition, antioxidant, antimicrobial and enzyme inhibition activities of two Origanum vulgare subspecies (subsp vulgare and subsp hirtum) essential oils
(ELSEVIER SCIENCE BV, 2015) Sarıkürkcü, Cengiz; Zengin, Gökhan; Oskay, Mustafa; Uysal, Şengül; Ceylan, Ramazan; Aktümsek, Abdurrahman
Essential oils (EOs) from Origanum vulgare subsp. vulgare (OW) and O. vulgare subsp. hirtum (OVH) were evaluated for antioxidant activities (DPPH, ABTS, FRAP, CUPRAC, beta-carotene/linoleic acid, phospho-molybdenum and metal chelating), antimicrobial and inhibitory properties against acetylcholinesterase, butrylcholinesterase, tyrosinase, alpha-amylase and alpha-glucosidase. Thymol and linalool were identified as major component in OW and OVH essential oils, respectively. O. vulgare subsp. vulgare exhibited a strong free radical scavenging, reducing power, antimicrobial and acetylcholinesterase, butrylcholinesterase, alpha-amylase and alpha-glucosidase inhibitory activity. Interestingly, OVH possess a significant metal chelating and tyrosinase inhibitory activities. The EOs of both species exhibited moderate antibacterial and antifungal activities. The MIC values of the EOs ranged from 85.3 to 426.7 mu g/mL. O. vulgare subsp. vulgare showed higher activity against Sarcina lutea with the lowest MIC (85.3 mu g/mL), whereas OVH indicated strong activity for Candida albicans with MIC value of 85.3 mu g/mL. The data suggest that the Origanum EOs could be used as valuable new natural agents with functional properties for food and pharmacology industries. (C) 2015 Elsevier B.V. All rights reserved.
Crepis foetida L. subsp rhoeadifolia (Bleb.) Celak. as a source of multifunctional agents: Cytotoxic and phytochemical evaluation
(ELSEVIER SCIENCE BV, 2015) Zengin, Gökhan; Sarıkürkcü, Cengiz; Uyar, Pembegül; Aktümsek, Abdurrahman; Uysal, Şengül; Koçak, Mehmet Sefa; Ceylan, Ramazan
The cytotoxic, anticholinesterase, antityrosinase and antioxidant effects of methanol extract from the flower of C. foetida subsp. rhoeadifolia (CFRME) were evaluated. The cytotoxic effect was investigated using HepG2, Caco-2, MCF-7 and MCF-10A cell lines. Enzyme inhibitory activities were tested by spectrophotometric methods. Different chemical assays were used to determine the antioxidant effects. In addition, phenolic constituents were quantified. High-performance liquid chromatographic (HPLC) analysis revealed that chlorogenic acid was the major phenolic component in the extract. The extract showed a strong antiproliferative, antioxidant, and anticholinesterase and antityrosinase effects. These findings suggest that C. foetida subsp. rhoeadifolia may be considered as a source of ingredients that can be used as food supplements. (C) 2015 Elsevier Ltd. All rights reserved.
Cytotoxic and Enzyme Inhibitory Potential of Two Potentilla species (P-speciosa L. and P-reptans Willd.) and Their Chemical Composition
(FRONTIERS MEDIA SA, 2017) Uysal, Şengül; Zengin, Gökhan; Locatelli, Marcello; Bahadori, Mir B.; Mocan, Andrei; Bellagamba, Giuseppe; De Luca, Elisa
In this work, the biological and chemical fingerprints of three extracts (ethyl acetate, methanol, and water) from two Potentilla species (Potentilla reptans and P. speciosa) were investigated. Antioxidant, enzyme inhibitory, and cytotoxic activities were performed for the biological fingerprint. For the chemical characterization, total bioactive components, and individual phenolic components were determined using photometric and HPLC methods, respectively. The main identified phenolic compounds in these extracts were rutin and catechin. Methanol and water extracts contained the highest total phenolic and flavonoid content. The results of antioxidant assays showed that methanol and water extracts displayed higher antioxidant activity compared to the ethyl acetate extract. Generally, methanol and water extracts exhibited higher biological activities correlated with higher levels the bioactive components. For P. speciosa, the methanol extract exhibited the highest enzyme inhibitory activity (except BChE inhibitory activity). P. reptans exhibited also high antiproliferative activity against MCF-7 cells whilst P. speciosa had weak to moderate activity against both of A549 and MCF-7 cell lines. The results suggest that Potentilla species could be potential candidates for developing new phyto-pharmaceuticals and functional ingredients.
Effects of Orange Leaves Extraction Conditions on Antioxidant and Phenolic Content: Optimization Using Response Surface Methodology
(TAYLOR & FRANCIS INC, 2018) Uysal, Şengül; Cvetanovic, Aleksandra; Zengin, Gökhan; Durovic, Sasa; Zekovic, Zoran; Aktümsek, Abdurrahman
In the last few years, bioactive components or their extraction techniques are gaining special interest in scientific areas. In this framework, orange leaves were used for preparation of extracts with high content of biologically active compounds. To optimize the extraction process, three levels and three variables of Box-Behnken design with response surface methodology were applied. Investigated responses were the total phenolic content (TPC), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, cupric ion reducing antioxidant capacity (CUPRAC), and ferric reducing antioxidant power (FRAP). Independent variables were methanol concentration (10-90%), temperature (20-60 degrees C), and extraction time (60-180min). Experimentally obtained results were fit into a second-order polynomial model with multiple regression. Analysis of variance was used to estimate model fitness and determine optimal conditions for processing. Estimated optimal conditions were 90% methanolic solution, 60 degrees C and 180min using these parameters; the predicted values of investigated responses were 43.19mg GAE/g (GAE: gallic acid equivalents), 43.04mg TE/g (TE: trolox equivalents), 139.34 and 93.76mg TE/g for TPC, DPPH, CUPRAC, and FRAP, respectively. The obtained optimal conditions could be considered as an alternative strategy for developing novel functional products.
Enzyme inhibitory effect and antioxidant properties of astragalus lagurus extracts
(Bentham Science Publishers B.V., 2016) Zengin, Gökhan; Ceylan, Ramazan; Güler, Gökalp Özmen; Carradori, S.; Uysal, Şengül; Aktümsek, Abdurrahman
Antioxidant and enzyme inhibitory effects (against cholinesterase, tyrosinase, ?-amylase and ?-glucosidase) of ethyl acetate, methanol, and aqueous extracts from Astragalus lagurus (Fabaceae) were investigated with spectrophotometric methods. Antioxidant capacity were evaluated by using different assay including free radical scavenging (DPPH and ABTS), reducing power (FRAP and CUPRAC), phosphomolybdenum, and metal chelating experiments. Total phenolic and flavonoid content was also determined as 20.34-20.72 mg GAEs/g and 19.58-31.10 mg REs/g, respectively. Generally, A. lagurus ethyl acetate and aqueous extracts were observed to possess higher antioxidant and enzyme inhibitory effects compared to methanol extract. The aqueous extract exhibited the strongest free radical scavenging activity on DPPH (20.65 mg TEs/g) and ABTS (67.43 mg TEs/g) as well as on reducing power tests (73.98 mg TEs/g for CUPRAC and 53.49 mg TEs/g for FRAP). ş-Amylase and ?-glucosidase inhibition were found to be 0.12-0.56 and 1.45-1.82 mmol ACAEs/g. These findings suggest that A. lagurus could provide natural biologically active agents to be used in food and pharmaceutical industry. © 2016 Bentham Science Publishers.
Essential Oil Composition of an Uninvestigated Centaurea Species from Turkey: Centaurea patula DC.
(TAYLOR & FRANCIS LTD, 2016) Zengin, Gökhan; Aktümsek, Abdurrahman; Boğa, Mehmet; Ceylan, Ramazan; Uysal, Şengül
Centaurea patula is widely distributed in the Central Anatolia Region of Turkey. The essential oil of C. patula was obtained by hydrodistillation and analyzed by GC-FID and GC-MS techniques. Twenty-one components were identified representing 86.4% of the oil. Spathulenol (14.6%), n-hexadecanoic acid (13.4%), 1-pentadecene (13.1%) and phytol (12.4%) were found to be the major components in this oil. This work is the first report on the chemical composition of the essential oil of C. patula. The results could be valuable for the Centaurea genus in terms of essential oil information's.
Evaluation of Antioxidant Potential, Enzyme Inhibition Activity and Phenolic Profile of Lathyrus Cicera and Lathyrus Digitatus: Potential Sources of Bioactive Compounds for the Food Industry
(ELSEVIER, 2017) Llorent-Martínez, Eulogio J.; Ortega-Barrales, Pilar; Zengin, Gökhan; Mocan, A.; Simirgiotis, M. J.; Ceylan, Ramazan; Uysal, Şengül
The genus Lathyrus has great importance in terms of food and agricultural areas. In this study, the in vitro antioxidant activity (phosphomolybdenum, DPPH, ABTS, FRAP, CUPRAC and metal chelating) and enzyme inhibitory activity evaluation (acetylcholinesterase, butyrylcholinesterase, alpha-amylase and alpha-glucosidase) of L. cicera and L. digitatus were investigated, as, well as their phytochemical profiles. The screening of the main phytochemical compounds in aerial parts of L. cicera and L. digitatus was carried out by high-performance liquid chromatography with electrospray ionization mass spectrometric detection (HPLC-ESI-MSn), observing that flavonoids represent the highest percentage of identified compounds, with abundance of tri- and tetra-glycosilated flavonoids, including acylated ones, especially in L. cicera. Generally, L. digitatus exhibited stronger antioxidant and enzyme inhibitory activities in correlation with its higher level of phenolics. The high number of phenolic compounds and the results of the antioxidant and enzyme assays suggest that these plants may be further used as sources of bioactive compounds, and for the preparation of new nutraceuticals. (C) 2017 Elsevier Ltd. All rights reserved.
Fatty Acid Composition, Total Sugar Content and Anti-Diabetic Activity of Methanol and Water Extracts of Nine Different Fruit Tree Leaves Collected from Mediterranean Region of Turkey
(TAYLOR & FRANCIS INC, 2015) Uysal, Şengül; Zengin, Gökhan; Aktümsek, Abdurrahman; Karataş, Şükrü
In this research, the total sugar content, fatty acid compositions, and -amylase and -glucosidase inhibitory activity of methanolic and water extracts of nine different fruit tree leaves were determined. -amylase and -glycosidase inhibitory activity were determined by using Caraway-Somogyi iodine/potassium iodide and 4-nitrophenyl--D-glucopyranoside as substrate, respectively. The total sugar content of the nine different fruit tree leaves varies from 281.02 mg GE/g (glucose equivalents) to 643.96 mg GE/g. Methanolic extract from avocado leaves had the strongest in -amylase and -glucosidase inhibitory activity, 69.21 and 96.26%, respectively. Fatty acid composition of nine fruit tree leaves was characterized by gas chromatography and 24 components were identified. Among the tested fruit tree leaves, the main component was linolenic acid (49.09%). The level of essential fatty acids are over 50% in mulberry, grape, and loquat leaves. Polyunsaturated fatty acids were the major group of fatty acids present in oils of mulberry, fig, pomegranate, grape, and loquat leaves. Therefore, these oils can be considered as a good source of polyunsaturated fatty acids. Furthermore, avocado can be regarded as a new source for diabetic therapies.
Functional constituents of six wild edible Silene species: A focus on their phytochemical profiles and bioactive properties
(ELSEVIER SCIENCE BV, 2018) Zengin, Gökhan; Mahomoodally, Mohamad Fawzi; Aktümsek, Abdurrahman; Ceylan, Ramazan; Uysal, Şengül; Mocan, Andrei; Yılmaz, Mustafa Abdullah
Six wild species (S. alba, S. conoidea, S. dichotoma, S. italica, S. supina, and S. vulgaris) from the Silene genera were tested for potential anti-enzymatic (acetyl cholinesterase (AChE), butyryl cholinesterase (BChE), tyrosinase, alpha-amylase, and alpha-glucosidase), antimicrobial (16 microbial strains), and antioxidant activity. An ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry method was used for phytochemical determination. Quinic acid, malic acid, protocatechuic acid, p-coumaric acid, and hesperidin were common in the six Silene species. All extracts showed higher antibacterial effects compared to streptomycin and ampicillin (except S. dichotoma). Antifungal agents, bifonazole (MIC 0.10-0.20 mg/mL and MFC 0.20-0.30 mg/mL) and ketoconazole (MIC 0.15-2.30 mg/mL and MFC 0.20-3.50 mg/mL) showed lower activity than the investigated Silene species extracts. S. alba inhibited AChE (2.00 mg GALAE/g extract) and BChE (1.0 mg GALAE/g extract). The results showed metal chelating potential ranging from 12 to 19 mg EDTAE/g extract, with S. conoidea being the most active, and S. supina the least. S. dichotoma showed the highest reducing potential against both cupric (154 mg TE/g extract for CUPRAC) and ferric (102 mg TE/g extract for FRAP) ions. Overall, Silene species could be considered as emerging interesting functional foods and sources of nutraceuticals with applications in the management of different diseases.

Yazar "Uysal, Şengül" seçeneğine göre listele

Sayfa Başına Sonuç

Sıralama seçenekleri