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Melatonin Differences Between Day and Night Milk in Primiparous Holstein Friesian and Jersey Dairy Cattle
(Selçuk Üniversitesi, 2022) Boztepe, Saim; Keskin, İsmail; Semacan, Ahmet; Akyürek, Fikret; Aytekin, İbrahim; Şahin, Özcan
This study was conducted to determine the levels of melatonin in the day and night milk of Holstein and Jersey cows. In the study, samples of daytime milk produced from 27 head of Holstein and 27 head of Jersey cows in the first lactation, which were raised in a private dairy cattle enterprise in the Kaşınhanı neighborhood of Meram district of Konya city Turkey, and night milk samples taken from the same cows that were blackened for one (1) week were used. Melatonin levels in milk samples taken from day and night milk were determined separately for Holstein and Jersey cows with the help of Bovine Melatonin (MLT) Elisa Kit. In the study, it was determined that the ratio of melatonin in day and night milk in Holstein cows was 2.912 pg/ml and 11.314 pg/ml, respectively, and the ratio of melatonin in Jersey cows was 2.924 pg/ml and 6.954 pg/ml in the same order. The difference between the melatonin levels of the day and night milk of Holstein and Jersey cows was found to be statistically significant (p<0.01). At the end of the study, it can be stated that night milk can be used for medical purposes and a new production source may arise for producers since there is a significant difference in melatonin between day and night milk.
Koyunlarda Ventral Trunkal Vagotominin Ön Mideler ve Abomasum Fonksiyonları Üzerine Etkileri
(Selçuk Üniversitesi, 1990 Ocak) Turgut, Kürşat; Başoğlu, Abdullah; Koç, Yılmaz; Çiftçi, Kemal; Tıpırdamaz, Saadettin; Aslan, Veysi; Gürbilek, Mehmet; Ok, Mahmut; Özkan, Cevat
In this study, a total of 12 sheep was used. 6 of which were used as a control group on which only paramedian laparatomy was performed. 6 of which were used as an experimental group on wihch ventral truncal vagotomy was performed via paramedian laparatoy. All the clinical, heamotological, biochemical and electrocardiographic examinations were performed for a month during the experiment. During the study clinical symptoms of vagus indigestion which has been described by Hoflund were not observed. There was significant decreaments in the respiration rate of experimental group at the 4 ^ (th), 10 ^ (th) and 20th day of the experiment. Plasma BE values were significantly increased at the 10 ^ (th) 20 ^ (th) and 30 ^ (th) day of axperiment in the experimental group. Rumen Cl concentration was significantly increased only at the 15 ^ (th) day of the experiment in the experimental group. Plasma gastrin concentration was decreased only at the sceond day of the experiment in the experimental group. Sinus arret was recorded during the electrocardiographic examination of the two groups of sheep at the diferent time. Theresult of the study showed that functional disorders of the nervus vagus did not play a role of the pathogenesis of vagus indigestion.
Köpeklerde Alloksan ile Oluşturulan Eksperi?mental Di?abetes Üzeri?nde Araştırmalar
(Selçuk Üniversitesi, 1992 Mart) Tiftik, Ali Muhtar; Turgut, Kürşat; Gürbilek, Mehmet; Sevinç, Mutlu
In this investigation, nine healthy dogs weighing from 9 to 20 kg were used. The dogs were made diabetic by a single intravenous injection of 0.60-0.70 mmol/kg bw of alloxan. Intravenous glucose tolerance tests (IVGTT) were done at the beginning of the study and in the second weeks of alloxan injections. At the beginning of the study, the mean values of fasted plasma glucose and insulin were found to be 4.56 ± 0.32 mmol/1 and 9.73 ± 2.69 mU/ml respectively. After the alloxan injections, clear hyperglycemia and decreasing insulin levels were determined and plasma insulin levels waried between 0-5.33 mU/ml during the study. In the urinalyses, glucosuria, ketonuria, proteinuria and decreased urinary pH levels were observed in the alloxan diabetic dogs. After diabetes occured, experimental diets (low high fibre diets) periode was started. Increasig plasma glucose levels were determined During the low fibre diet periode whereas decreasing glucose levels determined in the high diet periode. It is concluded that, it is necessary to apply the IVGTT for the certain diagnosis of diabetes and it is appropriate the recommend of rye bread for the regulation of hyperglycemia.
Köpeklerde Rompun- Ketalar ve Acepromazi?ne- Ketalar Kombi?nasyonlarının Kardi?opulmoner Etki?leri? Üzeri?nde Karşılaştırmalı Araştırmalar
(Selçuk Üniversitesi, 1993) İzci, Celal; Eksen, Mursayettin; Koç, Yılmaz; Keskin, Ercan; Kul, Mustafa; Avki, Sırrı; Gören, Mehmet
This study was carried out in two groups that consists of 5 dogs. Cardiopulmonary function was assessed in healty dogs that given xylazine-ketamine hydrochloride and acepromazine- ketamine hydrochloride combinations intramuscularly. Arterial blood pressure, heart rate and potassium demonstrated significant alterations statistically from pretest values. Arterial blood pressure (systolic, diastolic and mean) was significantly decreased by both combinations. The xylazine-ketamine hydrochloride combination decreased the heart rate 26.5 % in ratio, whereas the acep- romazine-ketamine hydrochloride combination increased it a maximum of % 67.1. The respiratory rate, body temperature, arterial pH. PaC*O_{2} PaO2 calcium and sodium values were not significantly altered.
Köpeklerde Akut Di?goksi?n Toksi?kasyonunda Kolesti?rami?n'i?n Etki?si?
(Selçuk Üniversitesi, 1993) Başoğlu, Abdullah; Maden, Mehmet; Çiftçi, Kemal; Akkuş, İdris; Koçyiğit, Abdurrahim; Hatipoğlu, Fatih; Akbulut, Hüseyin
In this study, the effects of cholestyramine on the clinical, laboratory and histopathologic findings in dogs with acute digoxin toxicosis was induced by intravenouse injection of digoxin at the dose of 0.1 mg/kg in two groups of dogs (control and experimental groups) and clinical pathologic, histopathologic and electrocardiographic findigs and changes in serum digoxin concentrations were evaluated. Incriments in red blood cell count (RBC), blood urea nitrogen (BUN) and serum uric acide. Potassium and digoxin concentrations and decreaments in serum sodium and chlorid concentrations were found to be significant in control group of dogs while changes in this parameters were not found to be significant in experimental group of dogs treated with cholestyramine. At the histopathologic examinataions of both groups of dogs, the pathologic lesions in coroner arteries, myocardium and kidneys in control group of dogs were more severe than that of experimental group of dogs and a dog in control group died during the experiment. The result of the study showed that acute digoxin toxicosis caused significant changes clinical pathologic and histopathologic significant changes, clinical pathologic and histopathologic findings and the treatment of acute digoxin toxicosis with cholestyramine would be benefical in dogs.
I?zmi?r Bölgesi? Koyunlarında Kan Serumu Bakır (Cu), Demi?r (Fe) Total Demi?r Bağlama Kapasi?tesi? (TDBK) ve Çi?nko (Zn) Düzeyleri?ni?n Araştırılması
(Selçuk Üniversitesi, 1997 Ocak) Tiftik, Ali Muhtar; Doğanay, Selma Çınar
In this research designed for determining Cu, Fe, Total iron binding capacity (TIBC) and Zn levels of blood serum of the healthy ewes grazed in the meadows in Izmir Region, a total of 250 units of blood-samples, being from at least 10 ewes in each herd, were collected from different 15 herds belonging to various villages of some different counties of Izmir (Karşıyaka, Seferihisar, Torbalı, Kemalpaşa, Uria) After eliminated sera that shows haemolyses, cop per (Cu), Iron (Fe), Total Iron Binding Capacity (TIBC) and Zinc levels were analyzed by atomic absorption spectrophotometer in 210 sera.In the assessments respect to serum Cu, Fe, TIBC and Zn leves, considerable diversitiesbeing at p< 0.01 levels-from the statistical point of view were observed between the average values of the counties and herds Although there were considerable differences from county to county, the average values of these counties and herds that indicated the lowest values in respects of iron and zinc contents, were found to be either similar to or just over the normal values. Therefore, it can be stated that the meadows of the counties in questions are rich enough in the elements investigated, and so, there is no need for adding those elements in to ewes rations. In the result of the assessment considering only the copper content, the average serum copper-level of the ewes from Torbalı County of Izmir was found to be remarkably lower, with a rate of p<0.01, than those from other counties. This level is actually lower than the limits of normal values. The average values of only one among the herds in Torbals and 54.54% rate of the entire ewes when considered the individual values, have been found to be lower than the deficiency limit. Under the light of all these findings, taking apart the fact that no soil and plant analyses were executed during the research, we can claim that the meadow soil and plant levels in this county lack in copper and the rations of those animals grazing at meadows are required to be reinforced with copper content.
Rasyona Katılan Vitamin ve İz Mineral Karmalarının Kuzularda Besi Performansı, Kan Plazması, Yapağı Kalitesi ve Sindirilme Derecesi Üzerine Etkileri
(Selçuk Üniversitesi, 1998) Coşkun, Behiç; Tiftik, Ali Muhtar; İmik, Halit; Aytaç, Melik
In this study. it was aimed to search ıhe eHect ol vitamin and trace minerals on the laııening perlormance, blood plasma. wool quality, and digestibility in lambs. Thirty live. about 2.5 morıths·old, Akkaraman lambs were used for 56 days. The search was conducted with 5 dillereni gfOUpS. Vitamin and mineral premix were 001 added lo the diels ol lirs! group, commercial level of vitamin premix were added to the diels ol second group (V), commercial level ol mineral premix were added to third group (M), both vitamin and mineral premixes were added together ıo the diets ol lorth group (VM), half commercial level ol both premixes were added to the diels ol lilth group (VM/2). The tem· peratures varied from 24 to 36 C during the study. In K, V, M, VM and VMl2 groups average daily live weight gains were 241,271,368,350 and 361 gram respectively. The groups to wtıich mineral premix were added gained more weight lhan the other groups significantly (P<0.05). Atter shearing thickness in the samples obtained from HIp area were 22.69, 27.71, 27.56, 27.60 and 27.30 ( respectively. In addiıion dirty and elean wool weights, heights and on· dulation rate were determined. At the end ol lailening period, the levels ol vitamin A in blood sera samples were 81.30,100.19, 100.96,80.54 and 81.52 d1dl; 175.21, 198.93,269.46,236.43 and 2S1.3g dldl for Zn; 172.68, 152.50,172.68.152.50, 127.00.127.14 and 107.22 dldl lor Cu respectively. As a resull, adding the mineral pre· mixes in the lamb laııening diels caused lo increase in body weight and tend to increased in thickness ol the wool.
Sütçü Sığırlarda Doğum Öncesi Doğum ve Doğum Sonrası Lipid ve Lipoprotein Konsantrasyonları
(Selçuk Üniversitesi, 1998) Sevinç, Mutlu; Başoğlu, Abdullah; Birdane, Fatih; Öztok, İsmail; Yenice, Mustafa
In this study, cholesterol, lriglyceride; HDL, LDL and VLDL concenlrations in sera were analysed from Ihe sevenlh monlh ol pregnancy lo lhe second monlh ollaclalion in !3 Holslein dairy cows. Triglyceride and VLDL concenlraıions were signilicantly elevaıed (p
Biochemical and hematological profiles of wistar rats at the Selcuk University experimental medicine research and application center
(Selçuk Üniversitesi, 2021) Öztürk, Bahadır; Çiftçi, İlhan; Ecer, Büşra; Gökyaprak, Salih Metin; Onmaz, Duygu Eryavuz
Aim: Breeders provide a set of reference values for the specific populations, however, rats produced in local experimental animal research centers may have undergone physiological and genetic changes. Our aim in this study was to contribute to further studies by investigating the biochemical and hematological profile of Wistar rats raised under standard local conditions at the Selcuk University Experimental Medicine Research and Application Center (SUDAM), Konya, Turkey. Materials and Methods: The study included 1 (male=15, female=15), 3 (male=15, female=15), 6 (male=15, female=15), and 12-month-old (male=15, female=15) Wistar rats living their normal lives in the Animal House of SUDAM. Selected biochemical and hematological parameters were measured with the Beckman AU 5800 and Beckman Coulter LH-780 analyzers, respectively. Results: Urea, creatinine, ALT, albumin, globulin, RBC, hemoglobin, hematocrit, and MCH levels differed significantly between rats according to months (p<0.05). When the selected biochemical and hematological parameters were compared according to gender, it was found that glucose, urea, ALP, amylase, albumin, total protein, CK, cholesterol, triglyceride, sodium, potassium, RBC, hematocrit, MCH, platelet, WBC, neutrophil, monocyte, eosinophil, basophil levels showed a statistically significant difference (p<0.05). Conclusion: This study proved that biochemical and hematological parameters of Wistar rats may alter with factors such as age, gender, environment, and nutrition.
The Relationship Between Plasma MicroRNAs and Serum Mercury Levels in Patients with Amalgam Filling and Dentists
(Selçuk Üniversitesi, 2021) Tunçdemir, Makbule Tuğba; Yerlikaya, Fatma Hümeyra
Background: The aim of this study was to investigate the relationship between serum mercury and some plasma microRNA (miRNA) levels associated with neurological diseases in patients with amalgam filling and dentists. Methods: This study included 30 patients with amalgam filling, 30 dentists, and 30 healthy individuals as the control group. Circulating plasma miRNAs (124-3p, 125-5p, and 127-3p) were evaluated using real-time quantitative polymerase chain reaction analysis. The serum mercury levels were measured using inductively coupled plasma-mass spectrometry. ANOVA and Tukey’s multiple comparison tests were used for statistical analyses (? =.05). Results: A significant difference in serum mercury and plasma miRNA levels was found between the groups. Significant positive correlations between serum mercury and plasma miRNA 125-5p and 127-3p levels were detected in the patient group (r: 0.56 and r: 0.39, respectively). Serum mercury and plasma miRNA-125-5p levels showed a positive correlation in the dentist group (r: 0.37). Conclusion: Having amalgam filling caused a significant increase in serum mercury and some plasma miRNA levels (124-3p, 125-5p, and 127-3p). The presence of a positive correlation suggests that sensitivity to neurological diseases may increase because of mercury exposure. Alternative restorative materials should be preferred for amalgam filling and amalgam fillings should be replaced with other restorative materials under necessary preventions.
Comparison of the Results of Sodium, Potassium, and Chlorine Measured Through Ion Selective Electrode (ISE) Method in Different Devices
(Selçuk Üniversitesi, 2022) Akdağ, Turan; Kader, Saadet; Dost, Hüseyin; Ünlü, Ali
Objective: The measurements of electrolytes are an indispensable part of the routine tests performed in clinical biochemistry laboratories. In the present study, we aimed to evaluate Na, K, and Cl electrolytes with the ISE method in the Mindray BS-800M and Beckman Coulter AU5800 devices. Material and methods: The serum samples were obtained from the healthy volunteers admitted to the Karapınar State Hospital for control purposes between January and April 2021. The sample probe was conducted using the same indirect method in the Mindray BS-800M and Beckman Coulter AU5800 devices, which is an individual sample probe for the ISE method. Results: While the comparative measurements were performed on different devices, the correlations between the electrolytes were found as follows: Na [r=0.964, 95% CI (0.52-0.90)], K [r=0.995, 95% CI (0.09-0.12)], and Cl [r=0.972, 95% CI (0.69-1.12)]. Moreover, a correlation was also found between these results. Conclusion: A consistency between the measured Na, K, and Cl electrolytes was determined on the different analyzers. Also, a positive correlation was determined between those electrolytes. We consider that further and comprehensive studies are needed to elucidate these results.
Increased Serum Kynurenine/Tryptophan Ratio in Rats Fed Added Sugar
(Selçuk Üniversitesi, 2022) Öztürk, Bahadır; Onmaz, Duygu Eryavuz
Aim: The consumption of added sugars containing fructose has increased dramatically. Various studies have revealed that added sugar consumption may be involved in the pathogenesis of cardiovascular, metabolic, and neurocognitive disorders by triggering subclinical inflammation. The imbalance in the kynurenine pathway metabolites may be associated with inflammation and oxidative stress. This study aims to investigate the effect of high-fructose corn syrup-55 (HFCS-55), invert sugar and sucrose intervention on the kynurenine pathway metabolite levels (tryptophan, kynurenine, 3-hydroxykynurenine, 3-hydroxyanthranilic acid, quinolinic acid, and kynurenic acid) in Wistar rats. Material and Methods: Twenty-four Wistar male rats (8-12 weeks old, weighing 300-350 g) were included in the study. After one week of conditioning, the animals were randomly divided into four groups: chow diet and tap water (control, n = 6), chow diet and tap water including 10% HFCS-55 (55% sucrose, 45% glucose), chow diet and tap water including 10% sucrose, chow diet and tap water including 10% invert sugar (33% sucrose, 66% glucose and fructose). At the end of the 3-month experimental period, serum kynurenine metabolites levels were measured by tandem mass spectrometry. Results: Serum kynurenine levels and kynurenine/tryptophan ratio were significantly higher (p<0.05) and serum kynurenic acid levels were significantly lower (p<0.05) in rats fed with HFCS, sucrose and invert sugar compared to the control group. Conclusion: Our findings suggest that consumption of added sugar may lead to an imbalance in the kynurenine pathway metabolites. The altered kynurenine metabolism may trigger inflammation and oxidative damage, and may predispose to chronic diseases.
Biochemical and hematological profiles of wistar rats at the Selcuk University experimental medicine research and application center
(Selçuk Üniversitesi Veterinerlik Fakültesi, 2021)
Amaç: Yetiştiriciler, belirli popülasyonlar için bir dizi referans değeri sağlar, ancak yerel deneysel hayvan araştırma merkezlerinde üretilen sıçanlar fizyolojik ve genetik değişikliklere uğramış olabilir. Bu çalışmadaki amacımız, Konya, Türkiye, Selçuk Üniversitesi Deneysel Tıp Araştırma ve Uygulama Merkezi'nde (SUDAM) standart yerel koşullarda yetiştirilen Wistar ratların biyokimyasal ve hematolojik profillerini araştırarak gelecekteki çalışmalara katkıda bulunmaktır. Gereç ve Yöntem: Çalışmaya SUDAM Hayvan Evinde normal hayatlarını sürdüren 1 (erkek = 15, dişi = 15), 3 (erkek = 15, dişi = 15), 6 (erkek = 15, dişi = 15) ve 12 (erkek = 15, dişi = 15) aylık Wistar ratlar dahil edildi. Seçilen biyokimyasal ve hematolojik parametreler sırasıyla Beckman AU 5800 ve Beckman Coulter LH-780 analizörleriyle ölçüldü. Bulgular: Üre, kreatinin, ALT, albümin, globulin, RBC, hemoglobin, hematokrit ve MCH düzeyleri arasında aylara göre istatiksel olarak anlamlı bir farklılık vardı (p<0.05). Seçilen biyokimyasal ve hematolojik parametreler cinsiyete göre karşılaştırıldığında glikoz, üre, ALP, amilaz, albümin, total protein, CK, kolesterol, trigliserit, sodyum, potasyum, RBC, hematokrit, MCH, trombosit, WBC, nötrofil, monosit, eozinofil, bazofil düzeyleri arasında istatistiksel olarak anlamlı bir farklılık vardı (p<0.05). Öneri: Bu çalışma, Wistar ratların biyokimyasal ve hematolojik parametrelerinin yaş, cinsiyet, çevre ve beslenme gibi faktörlerle değişebileceğini kanıtlamıştır.
The relationship between serum clozapine concentrations and hematological parameters by a validated mass spectrometric method
(Elsevier B.V., 2020) Kamil Gharab, Karam Mazin; Onmaz, Duygu Eryavuz; Abuşoğlu, Sedat; Aydın, Memduha; Sivrikaya, Abdullah; Tok, Oğuzhan; Abuşoğlu, Gülsüm; Ünlü, Ali
Objective: Clozapine is one of the most effective drugs for resistant schizophrenia, but its severe metabolic and hematological side effects limit the use of clozapine. It has been reported that clozapine blood concentrations should be maintained between 350?600 ng/mL. Our aim was to develop a determination method for clozapine and its main metabolites norclozapine and clozapine-N-oxide, to perform validation studies and to investigate the change of various biochemical parameters in patients using clozapine. Methods: A liquid chromatography-tandem mass spectrometry (LC–MS/MS) method was developed and validated for clozapine measurement. Thus, blood samples were collected from 38 patients with schizophrenia and 32 healthy volunteers. Biochemical and hematological parameters were measured by Beckman-Coulter AU 5800 (Beckman Coulter, Brea, USA) and Beckman Coulter LH 780 analyzer (Beckman Coulter, Miami, FL, USA), respectively. Hormone levels were analyzed using Cobas 6000 analyzer (Roche Diagnostics, Germany). Results: The LC[sbnd]MS/MS method was linear between 1.22?2500 ng/mL (r2 = 0.9971) for clozapine. The retention times of clozapine, norclozapine and clozapine-N-oxide were 0.92, 0.89 and 0.95, respectively. Blood glucose (GLU) (p = 0.025), low density lipoprotein (LDL-cholesterol) (p = 0.015), triglyseride (TG) (p = 0.042) and total cholesterol (TC) (p = 0.024) levels were higher; hemoglobin (HGB) (0.015), mean corpuscular hemoglobin (MCH) (0.036), red blood cell count (RBC) (0.020), neutrophil (NEU) (0.034), and platelet (PLT) (P = 0.005) levels were lower in the clozapine group. Conclusions: This LC–MS/MS method was rapid, simple, cost-effective and suitable for the routine clozapine monitoring. Furthermore, norclozapine and clozapine-N-oxide were also determined. Monitoring of metabolic and hematological parameters with clozapine levels is very important. However, the limitations of the study were that the method was not validated for norclozapine and clozapine-N-oxide, so the validation parameters were not evaluated for these two metabolites. © 2019 Elsevier B.V.
Total methylated arginine load as a risk parameter in subjects with masked hypertension
(TAYLOR & FRANCIS INC, 2020) Hoşaf, Müşerref; Abuşoğlu, Sedat; Avcı, Ahmet; Demir, Kenan; Ünlü, Ali; Eryavuz, Duygu; Abuşoğlu, Gülsüm
Asymmetric dimethylarginine, symmetric dimethylarginine, and L-monomethylarginine are originated from the subsequent proteolytic catalysis of methylated arginine residues on different proteins and inhibit the endogenous nitric oxide generation. The changes in total methylarginine load (Asymmetric dimethylarginine plus symmetric dimethylarginine plus L-monomethylarginine) may contribute to hypertension. The aim of this study was to determine serum methylarginine concentrations in patients with masked hypertension and determine the association between these biomarkers and blood pressure measurements. Control group, masked hypertension and hypertension groups consisted of 40 subjects (11 males, 28 females, mean age 48.6 +/- 13.1), 28 subjects (14 males, 14 females, mean age 50.9 +/- 11.0) and 36 subjects (15 males, 21 females, mean age 54.4 +/- 12.3 years), respectively (P= 0.149). Serum total methylarginine load was significantly higher in hypertension group (0.63 +/- 0.23) compared to masked hypertension (0.49 +/- 0.16) and control groups (0.38 +/- 0.13) (P= 0.008 and P< 0.001). While there was no statistically significant difference between healthy control groups [0.147 (0.03-0.29)] and masked hypertension patients [0.144 (0.05-0.42)] for serum symmetric dimethylarginine levels (P= 0.970), it was markedly elevated in hypertension group [0.25 (0.07-0.54)] compared to masked hypertension group [0.14 (0.05-0.42)] (P= 0.001). Serum total methylarginine load was positively correlated with night-time SBP (r = 0.214, P= 0.029). Serum methylarginine levels might be a useful marker for determining the courses of clinical hypertension.
Determination of melatonin deprivation impact on sepsis with acute phase reactants
(ACADEMIC PRESS INC ELSEVIER SCIENCE, 2020) Akbulut, Hatice Feyza; Vatansev, Hüsamettin; Sekmenli, Tamer
Background: The aim of the present study is to determine the association of melatonin hormone level on CRP, Total Antioxidant Status, Leukocyte, Procalcitonin, and Malondialdehyde, all acute phase reactants in the dark and light cycle of rats with sepsis model. Materials and methods: In this study, 54 rats were divided into three groups. Whereas the first and third groups had a 12 h dark-light cycle, the second group was exposed to light for 24 h at 21 degrees C-22 degrees C for 10 d without any water and food restrictions. In the second and third groups, sepsis model was formed by cecal ligation and puncture (CLP) method at the end of 10th day, and blood samples were taken at the end of the 10th day. C-reactive protein, Malondialdehyde, Procalcitonin in the blood samples were analyzed by ELISA, and the levels of Total Antioxidant Status and leukocyte were determined by colorimetric method in the subsequent 12 and 24 h. Results: CRP values increased in the second group rats, which were kept continuously under light and had undergone CLP, from 288.8 mg/L to 584.0 mg/L at the end of the 12 h and the end of the 24 h, approximately, two times. In rats, which were kept under 12 h of light, 12 h of darkness, and applied CLP (group 3), these values increased from 416.9 to 619.1; an increase of 1.5 times. When assessed for MDA, it was determined that the differences between Group 2 and Group 3 were more prominent between 0 h and 12 h. While the MDA values in group 2 increased from 16.53 nmol/mL at the 12 h to 17.66 nmol/mL at the 24 h. However, MDA values did not yield statistically significant changes in the third group. Changes in the in PCT values were similar to the MDA values obtained. Increase coefficient of the PCT values between 0 h and 12 h in the second group 2 was 1.26; however, in the third group, it was negligible. Conclusions: An increase in the oxidative stress was observed in the rats that underwent CLP and melatonin deprivation via continuous 24 h light exposure for 10 d. Accordingly, deprivation of light is considered to be effective in sepsis treatment due to the increase in melatonin levels in intensive care unit patients. (C) 2019 Elsevier Inc. All rights reserved.
Association of depressive symptoms with 25(OH) vitamin D in hemodialysis patients and effect of gender
(SPRINGER, 2020) Yavuz, Yasemin Coşkun; Bıyık, Zeynep; Özkul, Durmuş; Abuşoğlu, Sedat; Eryavuz, Duygu; Dağ, Mikail; Körez, Muslu Kazım; Güney, İbrahim; Altıntepe, Lütfullah
Background Depression is common in chronic kidney disease ( CKD) patients and associated with significant increase in morbidity and mortality. In recent years, a relationship between vitamin D deficiency and depression has been shown. The aim of this study is to investigate the relationship between 25-hydroxy (OH) vitamin D and depression in hemodialysis patients. Methods A total of 140 patients were included in the study. Hamilton depression scale (HAM-D) was completed by all patients. 25(OH) vitamin D levels were compared between patients with and without depressive symptoms. Results Patients who had depressive symptoms had significantly lower 25(OH) vitamin D levels (13.70 [24.3-8.25] vs. 18.20 [29.2-11.7] ng/mL, p = 0.016). HAM-D score showed significant association with gender (p = 0.011) and 25(OH) vitamin D level (p = 0.011). Univariate logistic regression analysis showed that males had lower risk of depression by a ratio of 61.1% (OR 0.389, p = 0.012) and vitamin D-deficient patients had 2.88 times greater risk of depression compared to non-deficient patients (OR 2.885, p = 0.013). Multivariate logistic regression analysis showed that males had 53.7% less risk of depression (OR 0.463, p = 0.046) and vitamin D-deficient patients had 2.39 times greater risk of depression (OR 2.397, p = 0.047). When evaluated by gender, univariate logistic regression analysis showed that 25(OH) vitamin D and other variables were not associated with depression in females (p > 0.05), while only vitamin D level had a significant effect on depression in males (OR 8.207, p = 0.008). Conclusions We found a significant association between vitamin D level and depressive symptoms in hemodialysis patients. When analyzed according to gender, this association was found to stand independent of other variables only in males.
TNF-Alpha and IL-6 as biomarkers of impaired lung functions in dimethylacetamide exposure
(SCIENDO, 2019) Tutkun, Lütfiye; İritaş, Servet Birgin; Deniz, Serdar; Öztan, Özgür; Abuşoğlu, Sedat; Ünlü, Ali; Türksoy, Vugar Ali; Çetintepe, Sultan Pınar
Background: Tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) are well-known biomarkers of systemic inflammation that have been associated with many diseases in the past. In this study, we aimed to determine the relationship between impaired lung functions and the levels of these biomarkers in DMAc exposed people. Methods: 101 non-exposed control subjects (Group 1) and 109 DMAc-exposed workers from the polyvinyl chloride (PVC) industry were included in the study. In the next step, the exposed group was divided into two groups according to the level of exposure (Group 2 and 3). DMAc, TNF-alpha, IL-6, creatinine, ALT, AST, GFR and standard spirometry measurements were carried out in all subjects. Results: When compared to the control group, TNF-alpha and IL-6 levels were significantly high compatible with the increase of DMAc levels, in the exposed groups. Urinary DMAc Levels were 0.06 mg/L in the control group. This level is significantly low when compared to exposed and severely exposed group (2.43 mg/L and 3.17 mg/L). TNF-alpha levels were 56.86 pg/mL, 145.52 pg/mL and 230.52 pg/mL in control, exposed and severely exposed groups. IL-6 levels were found to be 38.08 pg/mL, 89.19 pg/mL and 116 pg/mL for control, exposed and severely exposed groups, respectively. Similarly, the FEV1/FVC ratio decreased especially in the severely exposed group (p 0.001). Conclusions: In our study, results have revealed that TNF-and IL-6 levels are promising biomarkers in the early diagnosis of lung function impairment in inhalational DMAc exposure.
The role of regulatory T cells in allergic rhinitis and their correlation with IL-10, IL-17 and neopterin levels in serum and nasal lavage fluid
(SPRINGER, 2020) Erkan, Kadriye.; Bozkurt, Mete K.; Artaç, Hasibe.; Özdemir, Hülya.; Ünlü, Ali.; Korucu, Emine N.; Elsürer, Çağdaş.
Purpose Allergic rhinitis (AR), is an IgE-mediated inflammation of the nose. Regulatory T cells (Tregs) and inflammatory cytokines have been shown to play a critical role in allergic airway inflammation. The aim of the study was to compare the levels of blood T lymphocyte subsets and IL-10, IL-17 and neopterin concentrations in serum and nasal lavage of patients with AR compared to healthy subjects. Methods The study included 38 subjects with moderate-severe AR and 36 sex- and age-matched controls. Peripheral blood CD3+, CD3+CD4+ and CD4+CD25+Foxp3 percentages were evaluated using flow cytometry. Levels of IL-10, IL-17 and neopterin were measured both in serum and nasal lavage fluid with ELISA and HPLC, respectively. Results No difference was found in the percentages of T lymphocyte subsets between the two groups (p > 0.05). Serum IL-10 levels were similar (p > 0.05), whereas nasal IL-10 was lower in AR subjects compared to control group (2.22 +/- 0.91 and 3.12 +/- 1.45 pg/ml, respectively) (p < 0.05). Mean serum and nasal IL-17 were higher in AR (107.7 +/- 79.61 and 527.36 +/- 738.7 pg/ml) than the control group (76.29 +/- 28.94 and 328.9 +/- 430.8 pg/ml) (p < 0.05 and p > 0.05). There were no significant differences in serum and nasal neopterin levels (p > 0.05). Conclusions Although there were no differences in the distribution of lymphocyte subsets between the AR and control groups, the finding of higher levels of serum and nasal IL-17 and lower levels of nasal IL-10 support the cytokine imbalance in the pathogenesis of AR.
The effect of allergen immunotherapy on serum periostin levels in children with allergic rhinitis
(BILIMSEL TIP YAYINEVI, 2019) Ucaryilmaz, Hulya; Emsen, Ayca; Dikener, Ahmet Hakan; Akdam, Neriman; Unlu, Ali; Artac, Hasibe
Objective: Periostin, an extracellular matrix protein, is related to the eosinophilic airway inflammation. There is no specific marker in allergen immunotherapy to evaluate clinical response. We aimed to investigate the serum periostin levels in the children who receive allergen immunotherapy. Materials and Methods: Sixteen patients between 8-18 years (12.7 +/- 2.8 years) with allergic rhinitis and/or asthma due to grass pollen hypersensitivity and 30 healthy subjects (11.7 +/- 2.6 years) were included. Demographic data, eosinophil counts, skin prick tests and the specific IgE levels of the patients are recorded. Symptom scores, visual analog scales, medication scores were determined and the serum periostin levels were measured in the beginning, 4th and 12th months of the allergen immunotherapy. Results: The symptom scores for rhinitis in the 4th month showed significant improvements in all of the patients (p< 0.05). Nine patients with allergic rhinitis accompanied by asthma, showed significant improvements in 12th month symptom score for asthma (p=0.018). A significant correlation was detected between the initial serum periostin levels and the symptom scores for the eye (r=0.668, p=0.005). No significant difference was found in serum periostin levels between the patient and the control groups. There were no significant differences in serum periostin levels in 4th and 12th months compared to the ones in the beginning. Conclusion: In this study, there were no significant differences in serum periostin levels of children during the allergen immunotherapy. The association of serum periostin levels with symptom scores for the eye needs to be confirmed in more children with allergic rhinoconjunctivitis.

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