I?ntestinal İskemi/Reperfüzyon Sonrası Diğer Organlardaki Oksidan Stresin Araştırılması
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Dosyalar
Tarih
2000
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
İskemi/reperfüzyon hasarlanmasında tetikleyici olarak sorumlu tutulan reaktif oksijen metabolitleri primer olarak meydana geldikleri organda hasarlanmaya neden olurken aynı zamanda diğer organ ve sistemleri de etkilemektedir. Intestinal iskemi reperfüzyon çalışmalarında akciğer, karaciğer, kalp ve kemik iliğinde bu etkiler gösterilmiştir. Çalışmamızda intestinal iskemi reperfüzyonda karaciğer akciğer ve böbrekte oksidan stres ölçüldü. Toplam 18 adet tavşan üç gruba ayrıldı. 1. grup (n=6) bir saatlik ileum iskemisi, 2. grup (n=6) bir saat iskemi bir saat reperfüzyon, 3. grup (n=6) sham grubu olarak çalışıldı. Deney sonunda tüm gruplardan karaciğer, akciğer ve böbrek doku örnekleri alındı. Doku homojenatlarında malondialdehid ve glutatyon düzeyleri ölçüldü. Her üç grubun sonuçları birbirleriyle karşılaştırıldı. Tüm dokularda reperfüzyon grubu malondialdehid düzeyleri sham grubundan anlamlı yüksekti (p<0,05). Glutatyon düzeyleri akciğer dokusunda gruplar arasında farksızken, karaciğer ve böbrek dokularında reperfüzyon grubunda sham grubundan anlamit düşüktü (p<0,05). Bu sonuçlar her üç dokunun da intestinal iskemi reperfüzyon sonrası oksidan stres altında kaldığını göstermektedir.
Reactive oxygen metabolites those accused of triggering the ischemia/reperfusion injury lead to tissue damage in the organ in which they are produced primarily. On the other hand they affect the other organs and systems. These effects in the lungs, liver, heart and bone marrow have been demonstrated in the intestinal ischemia/reperfusion studies. In our study: oxidant stress in liver, lungs and kidneys was investigated during intestinal ischemia/reperfusion. Totally 18 rubbits were divided into three groups. The first group was an hour of ileal ischemia, the second group was an hour of ischemia and an hour of reperfusion, the third one was sham group. Tissue samples from liver, lungs and kidneys were obtained when the procedures finished. Malondialdehyde and glutathione levels were measured in tissue homogenates. The results of the groups were compared with those of each others. Malondialdehyde levels of the reperfusion groups were significantly higher than those of sham groups in all tissues (p<0.05). There were no difference on the glutathione levels of the lungs tissue samples between the groups. Glutathione levels of the liver and kidneys tissue samples in the reperfusion group were significantly lower than that of shum group (p<0.05). These results revealed that, these three tissues are exposed to oxidant stress after intestinal ischemia/reperfusion.
Reactive oxygen metabolites those accused of triggering the ischemia/reperfusion injury lead to tissue damage in the organ in which they are produced primarily. On the other hand they affect the other organs and systems. These effects in the lungs, liver, heart and bone marrow have been demonstrated in the intestinal ischemia/reperfusion studies. In our study: oxidant stress in liver, lungs and kidneys was investigated during intestinal ischemia/reperfusion. Totally 18 rubbits were divided into three groups. The first group was an hour of ileal ischemia, the second group was an hour of ischemia and an hour of reperfusion, the third one was sham group. Tissue samples from liver, lungs and kidneys were obtained when the procedures finished. Malondialdehyde and glutathione levels were measured in tissue homogenates. The results of the groups were compared with those of each others. Malondialdehyde levels of the reperfusion groups were significantly higher than those of sham groups in all tissues (p<0.05). There were no difference on the glutathione levels of the lungs tissue samples between the groups. Glutathione levels of the liver and kidneys tissue samples in the reperfusion group were significantly lower than that of shum group (p<0.05). These results revealed that, these three tissues are exposed to oxidant stress after intestinal ischemia/reperfusion.
Açıklama
Anahtar Kelimeler
Biyokimya ve Moleküler Biyoloji, Intestinal ischemia/reperfusion, Liver, Lung, Kidney, Malondialdehyde, Glutathione, Intestinal iskemi/reperfüzyon, Karaciğer, Akciğer, Böbrek Malondialdehid, Glutatyon
Kaynak
Türk Biyokimya Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
25
Sayı
3
Künye
Çağlayan, F., Çağlayan, O., Çakmak, M., Günel, E., (2000). İntestinal İskemi/Reperfüzyon Sonrası Diğer Organlardaki Oksidan Stresin Araştırılması. Türk Biyokimya Dergisi, 25(3), 104-108.
