A new BODIPY/nanoparticle/Ni affinity system for binding of cytochrome c

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dc.contributor.authorMaltas, Esra
dc.contributor.authorKursunlu, Ahmed Nuri
dc.contributor.authorArslan, Gulsin
dc.contributor.authorOzmen, Mustafa
dc.date.accessioned2020-03-26T19:00:27Z
dc.date.available2020-03-26T19:00:27Z
dc.date.issued2015
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIn this study, 3,5-{Bis[4,4-difluoro, 8-(2,6-diethyl, 1,3,5,7-tetramethy1-4-bora-3a,4a-diaza-sindacene)]}benzoylchloride (BODIPY) was synthesized for the improving of a new immobilized metal affinity supporting material. Firstly, the synthesized BODIPY was immobilized on iron oxide superparamagnetic nanoparticles (SPIONs) and then, Ni(II) ions were chelated with the active terminals of BODIPY on nanoparticles surfaces to prepare an immobilized metal affinity (IMA) adsorbent for protein adsorption. The amount of BODIPY coated on SPIONs was about 29.7 mu M at 10 mg nanoparticles. 738 mu mol of Ni(II) ions were loaded to 10 mg of the SPIONs/BODIPY. The binding amount of cytochrome c was found to be 170 mu g to the SPIONs/BODIPY/Ni at pH 7.4. The binding amount of the molecules on SPIONs was analyzed by using UV-vis, fluorescence and atomic absorption spectroscopy. The characterization of the prepared surfaces was performed by FT-IR, SEM and TEM. (C) 2015 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipScientific Research Council of Selcuk UniversitySelcuk Universityen_US
dc.description.sponsorshipThis research was supported by Scientific Research Council of Selcuk University.en_US
dc.identifier.doi10.1016/j.apsusc.2015.05.082en_US
dc.identifier.endpage816en_US
dc.identifier.issn0169-4332en_US
dc.identifier.issn1873-5584en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage811en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.apsusc.2015.05.082
dc.identifier.urihttps://hdl.handle.net/20.500.12395/31771
dc.identifier.volume349en_US
dc.identifier.wosWOS:000357129100107en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.relation.ispartofAPPLIED SURFACE SCIENCEen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectBODIPYen_US
dc.subjectNanoparticlesen_US
dc.subjectAffinityen_US
dc.subjectProteinen_US
dc.subjectSeparationen_US
dc.titleA new BODIPY/nanoparticle/Ni affinity system for binding of cytochrome cen_US
dc.typeArticleen_US

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