Boğalarda Brovine Herpesvirus TİP-1 (BHV-1) Enfeksiyonunun Enzyme Linked İmmunosorbent Assay (ELISA), Polymerase Chain Reaction (PCR) ve Virus İzolasyonu (VI) Metotları ile Karşılaştırmalı Teşhisi ve Seroepidemiyolojisi
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Tarih
2004
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info:eu-repo/semantics/openAccess
Özet
Araştırmada, Konya ve çevresinden Konya Özel Konet mezbahasına kesim amacı ile getirilen, 100 adet sağlıklı görünümlü, yerli ırk ve 1 yaşındaki boğalardan kan, sperma ve nasal swap örnekleri alındı. Sperma ve nasal swap örnekleri Fötal Dana Böbrek (FDB) ve Madin Darby Bovine Kidney (MDBK) devamlı hücre kültürlerine inokule edildi, inkubasyon süresi sonunda 26 adet sperma ve 2 adet nasal swap örneğinde sitopatik etki (CPE) gözlendi, izole edilen virusların iden-tifikasyonu amacıyla Enzyme Linked Immunosorbent Assay (ELISA/Ag) kullanıldı ve sperma örneklerinden 15 adedi BHV-1 olarak identifiye edilirken, nasal swap örneklerinin hiçbirisi BHV-1 olarak identifiye edilemedi. Ayrıca sperma ve nasal swap örneklerinde BHV-1'in tespiti amacıyla ELISA ve Polymerase Chain Reaction (PCR) metodundan yararlanıldı. Direkt ELISA ile 1 adet sperma ve 1 adet nasal swap örneği pozitif bulundu. PCR ile ise 23 adet sperma örneği pozitif olarak belirlenirken, nasal swap örneklerinin hiç birinde pozitif sonuç tespit edilemedi. BHV-1'e karşı oluşan antikorların tespit edilmesi amacıyla ELISA/Ab ve serum mikronötralizasyon (serum mNT) testleri kullanıldı. BHV-1'e karşı oluşan antikorların tespiti amacıyla uygulanan ELISA/Ab ile 23 adet kan serumu pozitif olarak belirlenirken, serum mNT testi ile 14 adet kan serumu pozitif bulundu ve antikor pozitif bu örneklerin SN50 değerleri ise 1:1.78 -1:22.4 arasında tespit edildi.
In this study, blood, semen and nasal swap samples were taken from 100 clinically healty, indigenous and one year old bulls brought to Konya Private Konet abbatoir from Konya and its environment with the purpose of slaughter. Semen and nasal swap samples were inoculated into Foetal Bovine Kidney (FBK) and Madin Darby Bovine Kidney (MDBK) cell cultures. At the end of incubation, cytopatogenic effect (CPE) was determined in 26 semen and 2 nasal swap samples. Enzyme Linked Immunosorbent Assay (ELISA/Ag) was used for identification of viruses isolated. Two isolates from nasal swap could not be identified, while 15 out of 26 isolates from semen samples was identified as BHV-1 by ELISA. Following ELISA/Ag and Polymerase Chain Reaction (PCR) method was applied for detection of BHV-1 in semen and nasal swap samples. One semen sample and 1 nasal swap sample were found as positive by ELISA/Ag. No positive result could be detected in any of nasal swap samples while 23 semen samples were detected as positive by PCR. Besides, Enzyme linked immunosorbent assay (ELISA) and serum microneutralization test (serum mNT) were used for detection of antibodies aganist to BHV-1. Twenty-three blood sera samples were detected positive by ELISA, while 14 blood sera samples were detected positive by serum mNT. SN50 values of the positive sera samples were detected between 1:1.78-1:22.4.
In this study, blood, semen and nasal swap samples were taken from 100 clinically healty, indigenous and one year old bulls brought to Konya Private Konet abbatoir from Konya and its environment with the purpose of slaughter. Semen and nasal swap samples were inoculated into Foetal Bovine Kidney (FBK) and Madin Darby Bovine Kidney (MDBK) cell cultures. At the end of incubation, cytopatogenic effect (CPE) was determined in 26 semen and 2 nasal swap samples. Enzyme Linked Immunosorbent Assay (ELISA/Ag) was used for identification of viruses isolated. Two isolates from nasal swap could not be identified, while 15 out of 26 isolates from semen samples was identified as BHV-1 by ELISA. Following ELISA/Ag and Polymerase Chain Reaction (PCR) method was applied for detection of BHV-1 in semen and nasal swap samples. One semen sample and 1 nasal swap sample were found as positive by ELISA/Ag. No positive result could be detected in any of nasal swap samples while 23 semen samples were detected as positive by PCR. Besides, Enzyme linked immunosorbent assay (ELISA) and serum microneutralization test (serum mNT) were used for detection of antibodies aganist to BHV-1. Twenty-three blood sera samples were detected positive by ELISA, while 14 blood sera samples were detected positive by serum mNT. SN50 values of the positive sera samples were detected between 1:1.78-1:22.4.
Açıklama
Anahtar Kelimeler
Veterinerlik, Bovine Herpesvirus Tip-1 (BHV-1), ELISA, Nötralizasyon Testi, PCR ve Virus İzolasyon, Bovine Herpesvirus type-1 (BHV-1), Neutralization Test, PCR and Virus Isolation
Kaynak
Veteriner Bilimleri Dergisi. Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
20
Sayı
4
Künye
Bulut, O., Yavru, S., (2004). Boğalarda Brovine Herpesvirus TİP-1 (BHV-1) Enfeksiyonunun Enzyme Linked İmmunosorbent Assay (ELISA), Polymerase Chain Reaction (PCR) ve Virus İzolasyonu (VI) Metotları ile Karşılaştırmalı Teşhisi ve Seroepidemiyolojisi. Veteriner Bilimleri Dergisi. Eurasian Journal of Veterinary Sciences, 20(4), 61-70.
