Farklı antiseptik taşıyıcı ve kalsiyum hidroksit kombinasyonlarının doku çözücü etkinliğinin incelenmesi
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Dosyalar
Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu in vitro çalışmanın amacı farklı antiseptik taşıyıcı - kalsiyum hidroksit (KH) kombinasyonlarının sığır dokusu üzerindeki doku çözücü etkinliğini değerlendirmektir. Gereç ve Yöntemler: Yeni kesilmiş sığır damağından doku örnekleri elde edildi. Benzer şekilli 170 adet doku örneği hazırlandı. Örnekler önceden tartılan mikrosantrifüj tüplerine yerleştirildi ve 55 °C’de 18 saat kurutuldu. Başlangıç ağırlıkları hassas terazi ile ölçüldü. KH tozu; gliserin – distile su karışımı (7:1), Octenisept, Savlex, Klorheksidin (CHX) ya da sodyum hipoklorit (NaOCl) ile karıştırıldı. Hazırlanan kanal içi medikamentler (KM), doku örnekleri içeren tüplere yerleştirildi ve 20 sn vortekslendi. Örnekler 37 °C’de %100 nemlilikte KH+gliserin-distile su, KH+Octenisept, KH+Savlex, KH+CHX ya da KH+NaOCl karışımlarının içerisinde, 1, 3 ve 7 gün boyunca inkübe edildi, sonrasında 5 ml distile su ile yıkandı. Örnekler tekrar 55 °C’de 18 saat kurutuldu ve son kuru ağırlıkları ölçüldü. Doku ağırlık kaybı yüzdesi hesaplandı ve veriler tek yönlü varyans analizi ve Tukey testleri kullanılarak analiz edildi. Bulgular: KH+CHX, KH+NaOCl ve KH+Octenisept grupları, KH+Savlex ve KH+gliserin-distile su gruplarına göre sığır damak dokusunu daha iyi çözmüştür (P < 0.05). KH+Octenisept karışımı 3 günlük inkübasyon periyodunda daha iyi doku çözücü etkinlik sergilemiştir. Sonuç: KH’yi %5.25’lik NaOCl, %2’lik CHX ya da Octenisept ile karıştırarak kullanmak pulpa artıklarını çözmek bakımından klinisyenler için daha avantajlı olacaktır.
Background: The aim of this in vitro study was to evaluate tissue dissolution ability of various new antiseptic vehicle and calcium hydroxide combinations on bovine tissue. Methods: Tissue specimens were obtained from freshly dissected bovine palates. One hundred and seventy tissue samples of similar form were prepared. Specimens were placed in preweighted 1.5 ml microcentrifuge tubes and desiccated at 55°C for 18 hour. Initial dry weights were measured with an analytic balance. Calciumhydroxide powder were mixed as follows in Group 1: Glycerin with distilled water (7:1 ratio); Group 2: Octenisept; Group 3: Savlex; Group 4: 2% Chlorhexidine (CHX); or Group 5: NaOCl and filled into the tubes containing the tissues and vortexed for 20 seconds. Specimens were incubated at 37°C in 100% humidity for 1, 3 and 7 days and then were carefully rinsed with 5 mL distilled water and blotted dry. Samples were desiccated at 55°C for 18 hour and final dry weights were measured. Percentage of tissue weight loss was calculated and statistically analyzed by using one way analysis of variance and Tukey tests. Results: Mixing calcium hydroxide with chlorhexidine, 5.25% NaOCl and Octenisept dissolved the tissue more effectively than calcium hydroxide and Savlex and glycerin/water mixtures (P<0.05). Calcium hydroxide and Octenisept mixture demonstrated better tissue dissolving properties after 3 days of incubation. Conclusion: Mixing calcium hydroxide with 5.25% NaOCl, 2% CHX or Octenisept could be more advantageous for the clinicians in dissolving pulp tissue remnants.
Background: The aim of this in vitro study was to evaluate tissue dissolution ability of various new antiseptic vehicle and calcium hydroxide combinations on bovine tissue. Methods: Tissue specimens were obtained from freshly dissected bovine palates. One hundred and seventy tissue samples of similar form were prepared. Specimens were placed in preweighted 1.5 ml microcentrifuge tubes and desiccated at 55°C for 18 hour. Initial dry weights were measured with an analytic balance. Calciumhydroxide powder were mixed as follows in Group 1: Glycerin with distilled water (7:1 ratio); Group 2: Octenisept; Group 3: Savlex; Group 4: 2% Chlorhexidine (CHX); or Group 5: NaOCl and filled into the tubes containing the tissues and vortexed for 20 seconds. Specimens were incubated at 37°C in 100% humidity for 1, 3 and 7 days and then were carefully rinsed with 5 mL distilled water and blotted dry. Samples were desiccated at 55°C for 18 hour and final dry weights were measured. Percentage of tissue weight loss was calculated and statistically analyzed by using one way analysis of variance and Tukey tests. Results: Mixing calcium hydroxide with chlorhexidine, 5.25% NaOCl and Octenisept dissolved the tissue more effectively than calcium hydroxide and Savlex and glycerin/water mixtures (P<0.05). Calcium hydroxide and Octenisept mixture demonstrated better tissue dissolving properties after 3 days of incubation. Conclusion: Mixing calcium hydroxide with 5.25% NaOCl, 2% CHX or Octenisept could be more advantageous for the clinicians in dissolving pulp tissue remnants.
Açıklama
Anahtar Kelimeler
Antiseptik, endodonti, kalsiyum hidroksit, kanal-içi medikament, Antiseptics, endodontics, calcium hydroxide, root canal medicaments
Kaynak
Selçuk Üniversitesi Diş Hekimliği Fakültesi Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
4
Sayı
1
Künye
Akbulut, M. B., Güneşer, M. B., Eldeniz, A. Ü., (2017). Farklı antiseptik taşıyıcı ve kalsiyum hidroksit kombinasyonlarının doku çözücü etkinliğinin incelenmesi. Selçuk Üniversitesi Diş Hekimliği Fakültesi Dergisi, 4 (1), 1-5.
