Niazirin from Moringa oleifera Lam. attenuates high glucose-induced oxidative stress through PKC?/Nox4 pathway
dc.contributor.author | Wang, F. | |
dc.contributor.author | Bao, Y. | |
dc.contributor.author | Shen, X. | |
dc.contributor.author | Zengin, Gökhan | |
dc.contributor.author | Lyu, Y. | |
dc.contributor.author | Xiao, J. | |
dc.contributor.author | Weng, Z. | |
dc.date.accessioned | 2020-03-26T20:19:58Z | |
dc.date.available | 2020-03-26T20:19:58Z | |
dc.date.issued | 2019 | |
dc.department | Selçuk Üniversitesi | en_US |
dc.description.abstract | Background: Diabetic complications-coronary atherosclerosis is closely related to the increased reactive oxygen species (ROS) induced by hyperglycemia. ROS are reported to induce the abnormal proliferation of vascular smooth muscle cells (VSMCs) under high glucose conditions. Leaf and seed extracts from Moringa oleifera are found to exhibit antioxidant activity. However, few studies are evaluating the antioxidant activities of chemical compounds isolated from the M. oleifera especially in cardiovascular field. Purpose: The aim of this study is to explore the antioxidative effect during hyperglycemia of niazirin from M. oleifera. Study design: A cell model was applied. Methods: After the taking the in vitro antioxidant experiment including ferric reducing antioxidant power (FRAP), 2,2?-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) assay and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Cell viability was carried out using high glucose-induced VSMCs model. ROS production was tested by 2?,7?-dichlorofluorescein diacetate (DCF-DA) assay. The protein kinase C zeta (PKC?) and NADPH oxidase 4 (Nox 4) expression in vitro and in vivo were measured by western blot analysis. Results: Niazirin showed good free radical scavenging activity. Niazirin significantly attenuated the proliferation of high glucose-induced VSMCs. Furthermore, it could decrease the ROS and malondialdehyde (MDA) productions, while increased total antioxidant capacity (T-AOC), superoxide dismutase (SOD) as well as glutathione peroxidase (GPx) levels in high glucose-induced VSMCs and streptozotocin-induced mice. In addition, niazirin could eliminate the high glucose-induced PKC? activation, indicated by Thr410 phosphorylation and inhibition of the Nox4 protein expression in vitro and in vivo. Conclusion: Niazirin from M. oleifera exhibited notably antioxidant activities and could be utilized as a potential natural antioxidant in preventing diabetic atherosclerosis. © 2019 Elsevier GmbH | en_US |
dc.description.sponsorship | Priority Academic Program Development of Jiangsu Higher Education Institutions Natural Science Research of Jiangsu Higher Education Institutions of China: 18KJB550006 2016YFD0400200 Natural Science Foundation of Jiangsu Province: BK20180817 Priority Academic Program Development of Jiangsu Higher Education Institutions National Natural Science Foundation of China: 21476103, 31800280 | en_US |
dc.description.sponsorship | This work was financially supported by the N ational Natural Science Foundation of China ( 31800280 , 21476103 ), the Natural Science Foundation of Jiangsu Province of China ( BK20180817 ), the Natural Science Foundation of the Jiangsu Higher Education Institutions of China ( 18KJB550006 ). It was also supported in part by the National Key R&D Program of China ( 2016YFD0400200 ) and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD). | en_US |
dc.identifier.doi | 10.1016/j.phymed.2019.153066 | en_US |
dc.identifier.issn | 0944-7113 | en_US |
dc.identifier.pmid | 31447278 | en_US |
dc.identifier.scopusquality | Q1 | en_US |
dc.identifier.uri | https://dx.doi.org/10.1016/j.phymed.2019.153066 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12395/38463 | |
dc.identifier.wos | WOS:000652024800002 | en_US |
dc.identifier.wosquality | Q1 | en_US |
dc.indekslendigikaynak | Web of Science | en_US |
dc.indekslendigikaynak | Scopus | en_US |
dc.language.iso | en | en_US |
dc.publisher | Elsevier GmbH | en_US |
dc.relation.ispartof | Phytomedicine | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.selcuk | 20240510_oaig | en_US |
dc.subject | Diabetic atherosclerosis | en_US |
dc.subject | Moringa oleifera | en_US |
dc.subject | Natural antioxidant | en_US |
dc.subject | Niazirin | en_US |
dc.subject | Reactive oxygen species | en_US |
dc.title | Niazirin from Moringa oleifera Lam. attenuates high glucose-induced oxidative stress through PKC?/Nox4 pathway | en_US |
dc.type | Article | en_US |