Niazirin from Moringa oleifera Lam. attenuates high glucose-induced oxidative stress through PKC?/Nox4 pathway

Basit Öğe Kaydı
dc.contributor.authorWang, F.
dc.contributor.authorBao, Y.
dc.contributor.authorShen, X.
dc.contributor.authorZengin, Gökhan
dc.contributor.authorLyu, Y.
dc.contributor.authorXiao, J.
dc.contributor.authorWeng, Z.
dc.date.accessioned2020-03-26T20:19:58Z
dc.date.available2020-03-26T20:19:58Z
dc.date.issued2019
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractBackground: Diabetic complications-coronary atherosclerosis is closely related to the increased reactive oxygen species (ROS) induced by hyperglycemia. ROS are reported to induce the abnormal proliferation of vascular smooth muscle cells (VSMCs) under high glucose conditions. Leaf and seed extracts from Moringa oleifera are found to exhibit antioxidant activity. However, few studies are evaluating the antioxidant activities of chemical compounds isolated from the M. oleifera especially in cardiovascular field. Purpose: The aim of this study is to explore the antioxidative effect during hyperglycemia of niazirin from M. oleifera. Study design: A cell model was applied. Methods: After the taking the in vitro antioxidant experiment including ferric reducing antioxidant power (FRAP), 2,2?-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) assay and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Cell viability was carried out using high glucose-induced VSMCs model. ROS production was tested by 2?,7?-dichlorofluorescein diacetate (DCF-DA) assay. The protein kinase C zeta (PKC?) and NADPH oxidase 4 (Nox 4) expression in vitro and in vivo were measured by western blot analysis. Results: Niazirin showed good free radical scavenging activity. Niazirin significantly attenuated the proliferation of high glucose-induced VSMCs. Furthermore, it could decrease the ROS and malondialdehyde (MDA) productions, while increased total antioxidant capacity (T-AOC), superoxide dismutase (SOD) as well as glutathione peroxidase (GPx) levels in high glucose-induced VSMCs and streptozotocin-induced mice. In addition, niazirin could eliminate the high glucose-induced PKC? activation, indicated by Thr410 phosphorylation and inhibition of the Nox4 protein expression in vitro and in vivo. Conclusion: Niazirin from M. oleifera exhibited notably antioxidant activities and could be utilized as a potential natural antioxidant in preventing diabetic atherosclerosis. © 2019 Elsevier GmbHen_US
dc.description.sponsorshipPriority Academic Program Development of Jiangsu Higher Education Institutions Natural Science Research of Jiangsu Higher Education Institutions of China: 18KJB550006 2016YFD0400200 Natural Science Foundation of Jiangsu Province: BK20180817 Priority Academic Program Development of Jiangsu Higher Education Institutions National Natural Science Foundation of China: 21476103, 31800280en_US
dc.description.sponsorshipThis work was financially supported by the N ational Natural Science Foundation of China ( 31800280 , 21476103 ), the Natural Science Foundation of Jiangsu Province of China ( BK20180817 ), the Natural Science Foundation of the Jiangsu Higher Education Institutions of China ( 18KJB550006 ). It was also supported in part by the National Key R&D Program of China ( 2016YFD0400200 ) and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).en_US
dc.identifier.doi10.1016/j.phymed.2019.153066en_US
dc.identifier.issn0944-7113en_US
dc.identifier.pmid31447278en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.phymed.2019.153066
dc.identifier.urihttps://hdl.handle.net/20.500.12395/38463
dc.identifier.wosWOS:000652024800002en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier GmbHen_US
dc.relation.ispartofPhytomedicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectDiabetic atherosclerosisen_US
dc.subjectMoringa oleiferaen_US
dc.subjectNatural antioxidanten_US
dc.subjectNiazirinen_US
dc.subjectReactive oxygen speciesen_US
dc.titleNiazirin from Moringa oleifera Lam. attenuates high glucose-induced oxidative stress through PKC?/Nox4 pathwayen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu