Evaluation of the Triglyceride Composition of Pomegranate Seed Oil by RP-HPLC Followed by GC-MS

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dc.contributor.authorTopkafa, Mustafa
dc.contributor.authorKara, Huseyin
dc.contributor.authorSherazi, Syed Tufail Hussain
dc.date.accessioned2020-03-26T19:05:58Z
dc.date.available2020-03-26T19:05:58Z
dc.date.issued2015
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractTriglyceride composition and fatty acid profiles of pomegranate seed oil were evaluated by newly developed methods in reverse-phase-high performance liquid chromatography (RP-HPLC) and gas chromatography (GC), respectively. Different compositions of the mobile phase (acetone and acetonitrile) and flow rates for the HPLC system were used to obtain better separation for accurate quantitative analysis. Triglycerides with conjugated fatty acids (CLnAs) were eluted in order of the polarity of their geometrical isomers (c, t, c < t, t, c < t, t, t). The dominant triglyceride was found to be PuPuPu (32.99 %) in pomegranate seed oil, followed by PuPuCa and PuCaCa containing punicic acid and catalpic acid with total triglyceridelevels of 27.72 and 10.11 %, respectively. For fatty acid composition analysis, triglyceride fractions were derivatized into their respective methylesters which were injected into gas chromatography-mass spectrometry (GC-MS) to identify and gas chromatography-flame ionization detector (GC-FID) to quantify the conjugated fatty acids of each fraction of triglycerides. Punicic acid was found to be dominant (76.57 %) followed by catalpic acid (6.47 %) and beta-eleotearic acid (1.45 %). Pomegranate seed contained greater amounts of conjugated linolenic acids. These results showed that the present study provides more information about the composition of the triglyceride and fatty acid profiles of pomegranate seed oil compared to the reported studies. Therefore, the developed methods in this study can be used for the identification of the triglyceride and fatty acid composition for pomegranate seed oils and some such specials edible oils including CLnA isomers.en_US
dc.description.sponsorshipSelcuk University Coordinators of Scientific ResearchSelcuk University [SU-BAP 09101062]; TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TUBITAK 2221]en_US
dc.description.sponsorshipThis study, a part of PhD work entitled "Investigation of Refining Properties for Edible Pomegranate Seed Oil," was supported by the Selcuk University Coordinators of Scientific Research, project nos. SU-BAP 09101062. The authors wish to thank the principal of Selcuk University and Scientific Research Project Coordination and also TUBITAK for providing financial support to S.T.H. Sherazi under the TUBITAK 2221 Fellowship for Visiting Scientists and Scientists on Sabbatical Leave program.en_US
dc.identifier.doi10.1007/s11746-015-2652-1en_US
dc.identifier.endpage800en_US
dc.identifier.issn0003-021Xen_US
dc.identifier.issn1558-9331en_US
dc.identifier.issue6en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage791en_US
dc.identifier.urihttps://dx.doi.org/10.1007/s11746-015-2652-1
dc.identifier.urihttps://hdl.handle.net/20.500.12395/32139
dc.identifier.volume92en_US
dc.identifier.wosWOS:000354826300003en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSPRINGERen_US
dc.relation.ispartofJOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectPomegranate seed oilen_US
dc.subjectTriglyceride profileen_US
dc.subjectFatty acid compositionen_US
dc.subjectHPLCen_US
dc.subjectGC-FIDen_US
dc.subjectGC-MSen_US
dc.titleEvaluation of the Triglyceride Composition of Pomegranate Seed Oil by RP-HPLC Followed by GC-MSen_US
dc.typeArticleen_US

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